The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Analysis of Mitochondrial Gene Sequence in Etoxazole Resistant Two-Spotted Spider Mite, Tetranychus urticae

Etoxazole 저항성 점박이응애의 미토콘드리아 유전자 서열 분석

  • Park, Sang-Eun (Dept. of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Koo, Hyun-Na (Dept. of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Yoon, Chang-Mann (Dept. of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Choi, Jang-Jeon (Pear Research Station, NIHHS, RDA) ;
  • Kim, Gil-Hah (Dept. of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
  • 박상은 (충북대학교 농업생명환경대학 식물의학과) ;
  • 구현나 (충북대학교 농업생명환경대학 식물의학과) ;
  • 윤창만 (충북대학교 농업생명환경대학 식물의학과) ;
  • 최장전 (농촌진흥청 국립원예특작과학원 배시험장) ;
  • 김길하 (충북대학교 농업생명환경대학 식물의학과)
  • Received : 2011.11.07
  • Accepted : 2012.01.30
  • Published : 2012.03.31
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Abstract

The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is one of the most important pest species devastating many horticultural and ornamental crops and fruit trees. Difficulty in managing this mite is largely attributed to its ability to develop resistance to many important acaricides. Development of 3,700-folds resistance to etoxazole was found in the population of T. urticae collected from rose greenhouses in Buyeo, Chungnam Province in August 2000. This population has been selected for eleven years with etoxazole (over 500 times), and increased over 5,000,000-folds in resistance as compared with susceptible strain. Also, etoxazole-resistant strain was shown to be maternally inherited. The objective of this study was to determine whether resistance of T. urticae to etoxazole was linked with point mutations in the mitochondrial gene. DNA sequencing of cytochrome c oxidase subunit I (COX1), COX2, COX3, cytochrome b (CYTB), NADH dehydrogenase subunit 1 (ND1), ND2, ND3, ND4, ND5, and ND6 were analyzed by comparing two etoxazole-susceptible and etoxazole-resistant strains. As a result, differences were not detected between the nucleotide sequences of two strains within a mitochondrial gene.

점박이응애는 전 세계적으로 농업과 원예 분야에 경제적 손실을 일으키는 중요한 해충으로 많은 살비제에 대해 저항성이 발달하여 방제에 어려움을 겪고 있다. 2000년 8월 충남 부여의 장미 재배지에서 채집한 점박이응애가 etoxazole에 대해 3,700배의 저항성을 나타내었다. 이 집단을 실내에서 11년 동안 etoxazole로 500회 이상 도태하여 5,000,000배 이상의 저항성 계통을 얻었다. Etoxazole 저항성은 모계유전 하는 것으로 알려져 있다. 따라서 이들 etoxazole 저항성이 모계유전을 하는 미토콘드리아 유전자내 점 돌연변이와 관련이 있는지를 조사하였다. Etoxazole 저항성 계통과 감수성 계통의 CYTB, COX1, COX2, COX3, ND1, ND2, ND3, ND4, ND5, 그리고 ND6의 유전자 서열을 비교한 결과 저항성 계통에서의 점 돌연변이는 발견할 수 없었다.

Keywords

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