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http://dx.doi.org/10.7585/kjps.2012.16.1.054

Analysis of Mitochondrial Gene Sequence in Etoxazole Resistant Two-Spotted Spider Mite, Tetranychus urticae  

Park, Sang-Eun (Dept. of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Koo, Hyun-Na (Dept. of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Yoon, Chang-Mann (Dept. of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Choi, Jang-Jeon (Pear Research Station, NIHHS, RDA)
Kim, Gil-Hah (Dept. of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Publication Information
The Korean Journal of Pesticide Science / v.16, no.1, 2012 , pp. 54-61 More about this Journal
Abstract
The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is one of the most important pest species devastating many horticultural and ornamental crops and fruit trees. Difficulty in managing this mite is largely attributed to its ability to develop resistance to many important acaricides. Development of 3,700-folds resistance to etoxazole was found in the population of T. urticae collected from rose greenhouses in Buyeo, Chungnam Province in August 2000. This population has been selected for eleven years with etoxazole (over 500 times), and increased over 5,000,000-folds in resistance as compared with susceptible strain. Also, etoxazole-resistant strain was shown to be maternally inherited. The objective of this study was to determine whether resistance of T. urticae to etoxazole was linked with point mutations in the mitochondrial gene. DNA sequencing of cytochrome c oxidase subunit I (COX1), COX2, COX3, cytochrome b (CYTB), NADH dehydrogenase subunit 1 (ND1), ND2, ND3, ND4, ND5, and ND6 were analyzed by comparing two etoxazole-susceptible and etoxazole-resistant strains. As a result, differences were not detected between the nucleotide sequences of two strains within a mitochondrial gene.
Keywords
Tetranychus urticae; Etoxazole; Mitochondrial gene; Point mutation;
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