한국균학회지 (The Korean Journal of Mycology)

  • 제40권2호
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  • Pages.78-85
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  • 2012
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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Universal Rice Primer (URP)-PCR에 의한 곰팡이 종의 유전적 다양성 검정

Genetic Divesity Analysis of Fungal Species by Universal Rice Primer (URP)-PCR

  • 강희완 (한경대학교 바이오.정보기술대학원)
  • Kang, Hee-Wan (Graduate School of Bio.&Information Technology, Hankyong National University)
  • 투고 : 2012.06.04
  • 심사 : 2012.06.18
  • 발행 : 2012.06.30
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⟨ 이전 논문 다음 논문 ⟩

초록

20 mer의 URP primers 가 한국 재래적미의 반복배열 DNA 염기서열로부터 고안되어 다양한 곰팡이 종의 PCR 다형성검출에 적용 되어 왔다. URP-PCR 방법은 PCR반응중 $55^{\circ}C$이상의 고온에서 annealing반응을 함으로 하여 재생적인 PCR결과를 얻을 수 있으며 효모균류에서 담자균류의 고등 균류까지 33속, 142 종, 1,489 균주의 다양한 곰팡이 종의 유전체 DNA에 적용되어 그 유용성이 평가 되어 왔다. 본 논문은 URP-PCR의 특성과 지금까지 다양한 곰팡이 종 다양성 검정결과를 종합하여 보고 한다.

URP primers that were derived from repetitive DNA sequence of rice weedy rice have been applied for producing PCR polymorphisms in different fungal species. URP-PCR protocol employed stringent PCR with high annealing temperature over $55^{\circ}C$ throughout the thermo-cycling reaction, giving high reproducibility. Under the PCR condition, Each single URP primer produced characteristic fingerprints from diverse genomes of different fungal species, indicating its universal applicability. URP-PCR has been accessed for applicability to various fungal species with 33 genus, 142 species and 1,489 isolates. Numerous related papers have demonstrated that URP-PCR profiles of fungal species are very useful for identifying fungal species at intra and inter species levels. The results were reviewed in this paper.

키워드

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피인용 문헌

  1. Inter simple sequence repeat (ISSR)-PCR based polymorphism of Agaricus bisporus strains and monokayon isolates vol.13, pp.3, 2015, https://doi.org/10.14480/JM.2015.13.3.175
  2. Effect of Lime Sulfur on Changes of Fungal Diversity in Pear Fallen Leaves vol.43, pp.4, 2015, https://doi.org/10.4489/KJM.2015.43.4.281
  3. Genetic Diversity of Agaricus bisporus Strains by PCR Polymorphism vol.42, pp.1, 2014, https://doi.org/10.4489/KJM.2014.42.1.1