Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Expression of Recombinant Rotavirus Proteins Harboring Antigenic Epitopes of the Hepatitis A Virus Polyprotein in Insect Cells

  • Than, Van Thai (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Baek, In-Hyuk (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Lee, Hee-Young (Medikan Inc.) ;
  • Kim, Jong-Bum (Functional Biomaterial Division, National Academy of Agricultural Science, the Rural Development Administration) ;
  • Shon, Dong-Hwa (Food Immunology Research Group, Korea Food Research Institute) ;
  • Chung, In-Sik (Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Won-Yong (Department of Microbiology, Chung-Ang University College of Medicine)
  • Received : 2011.12.21
  • Accepted : 2012.03.20
  • Published : 2012.05.31
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Abstract

Rotavirus and hepatitis A virus (HAV) spread by the fecal-oral route and infections are important in public health, especially in developing countries. Here, two antigenic epitopes of the HAV polyprotein, domain 2 (D2) and domain 3 (D3), were recombined with rotavirus VP7, generating D2/VP7 and D3/VP7, cloned in a baculovirus expression system, and expressed in Spodoptera frugiperda 9 (Sf9) insect cells. All were highly expressed, with peak expression 2 days post-infection. Western blotting and ELISA revealed that two chimeric proteins were antigenic, but only D2/VP7 was immunogenic and elicited neutralizing antibody responses against rotavirus and HAV by neutralization assay, implicating D2/VP7 as a multivalent subunit-vaccine Candidate for preventing both rotavirus and HAV infections.

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