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http://dx.doi.org/10.4062/biomolther.2012.20.3.320

Expression of Recombinant Rotavirus Proteins Harboring Antigenic Epitopes of the Hepatitis A Virus Polyprotein in Insect Cells  

Than, Van Thai (Department of Microbiology, Chung-Ang University College of Medicine)
Baek, In-Hyuk (Department of Microbiology, Chung-Ang University College of Medicine)
Lee, Hee-Young (Medikan Inc.)
Kim, Jong-Bum (Functional Biomaterial Division, National Academy of Agricultural Science, the Rural Development Administration)
Shon, Dong-Hwa (Food Immunology Research Group, Korea Food Research Institute)
Chung, In-Sik (Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University)
Kim, Won-Yong (Department of Microbiology, Chung-Ang University College of Medicine)
Publication Information
Biomolecules & Therapeutics / v.20, no.3, 2012 , pp. 320-325 More about this Journal
Abstract
Rotavirus and hepatitis A virus (HAV) spread by the fecal-oral route and infections are important in public health, especially in developing countries. Here, two antigenic epitopes of the HAV polyprotein, domain 2 (D2) and domain 3 (D3), were recombined with rotavirus VP7, generating D2/VP7 and D3/VP7, cloned in a baculovirus expression system, and expressed in Spodoptera frugiperda 9 (Sf9) insect cells. All were highly expressed, with peak expression 2 days post-infection. Western blotting and ELISA revealed that two chimeric proteins were antigenic, but only D2/VP7 was immunogenic and elicited neutralizing antibody responses against rotavirus and HAV by neutralization assay, implicating D2/VP7 as a multivalent subunit-vaccine Candidate for preventing both rotavirus and HAV infections.
Keywords
Rotavirus; Hepatitis A virus; Recombinant chimera protein;
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