생명과학회지 (Journal of Life Science)

  • 제22권5호
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  • Pages.600-604
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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인천지역 급성 설사환자의 group A rotavirus 감염 실태 및 P와 G 유전자형 분포

The Prevalence and Distribution of the P and G Genotypes of a Group A Rotavirus Detected in Acute Gastroenteritis Patients from Incheon

  • Choi, Hye-Jin (Incheon Research Institute of Public Health and Environment) ;
  • Oh, Bo-Young (Incheon Research Institute of Public Health and Environment) ;
  • Lee, Mi-Yeon (Incheon Research Institute of Public Health and Environment) ;
  • Koh, Yeon-Ja (Incheon Research Institute of Public Health and Environment) ;
  • Gong, Young-Woo (Incheon Research Institute of Public Health and Environment) ;
  • Hur, Myung-Je (Incheon Research Institute of Public Health and Environment) ;
  • Lee, Jea-Mann (Incheon Research Institute of Public Health and Environment) ;
  • Kim, Young-Hee (Incheon Research Institute of Public Health and Environment) ;
  • Jeong, Hye-Sook (Korea Center for Disease Control & Prevention) ;
  • Cheon, Doo-Sung (Korea Center for Disease Control & Prevention)
  • 투고 : 2012.02.08
  • 심사 : 2012.03.22
  • 발행 : 2012.05.30
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초록

인천지역 병 의원에 내원한 급성위장염환자의 대변 검체 총 11,607건을 효소 면역법(ELISA)을 이용하여 group A rotavirus의 계절별 발생 양상을 조사한 결과 2005년부터 2010년간 인천지역에서는 1~2월이 가장 많이 발생하였으나, 기간별로 차이가 존재했다. 또, group A rotavirus 양성 검체 160건에 유전자 분석 결과 VP4는 P8형, VP7는 G1형, G와 P 조합형으로는 G1P8이 가장 많았다. 그러나 이전의 연구와 비교했을 때 연구 지역과 기간에 따라 혈청형과 유전형의 변화가 빈번하였으므로 효율적인 방역을 위해 지속적인 모니터링이 필요하다고 사려된다. 또 이번 연구는 현재 유통 중인 rotavirus 백신의 효율적인 사용의 기초 자료가 될 것이다.

Rotavirus is the main cause of severe diarrhea in infants and young children of the world. However, the frequency of genetic alterations makes it hard to control the prophylaxis. Therefore, continuous monitoring of the rotavirus's genetic change is inevitable to prevent disease prevalence and is useful in inventing an efficient vaccine. From January 2005 to December 2010, we investigated 11,607 stool samples of acute gastroenteritis patients in the Incheon metropolitan area. About 13.18% (1,530 stool samples) of all samples had a positive reaction against rotavirus using an antigen capture enzyme-linked immunosorbent assay (ELISA). Then, the 160 stool samples were searched for subtypes of group A rotavirus by using a reverse transcription polymerase chain reaction (RT-PCR) and a nested multiplex RCR. In P sub-typing, P8 (56.3%) was an extremely prevalent genotype, followed by P6 (21.3%), and P1A (10.0%). G1 (39.4%) was most widespread in the G subtype, followed by G4 (25.0%) and G3 (18.8%). G1P8 (35.5%) was the most common G and P subtype combination, followed by G4P6 (19.3%) and G3P8 (13.1%). These results might be useful data for understanding the epidemiological status of group A-rotavirus dispersion in the Incheon metropolitan area.

키워드

참고문헌

  1. Barnes, G. L., Uren, E., Stevens, K. B. and Bishop, R. F. 1998. Etiology of Acute gastroenteritis in hospotalized children in Melbourne, Australia, from April 1980 to March 1993. J. Clin. Microbiol. 1, 133-138.
  2. Beards, G. M., Desselberger, U. and Flewett, T. H. 1989. Temporal and geographical distributions of human rotavirus serotype, 1983 to 1988. J. Clin. Mirobiol. 27, 2827-2833.
  3. Bishop, R. F., Davidson, G. P., Holmes, I. H. and Ruck, B. J. 1973. Detection of a new virus by electron microscopy of fecal extracts from children with acute gastroenteritis. Lancet 1, 149-151.
  4. Brandt, C. D., Kim, H. W., Rodriguez, W. J., Arrobio, J. O., Jeffries, B.C., Stallings, E. P., Lewis, C., Miles, A. J., Chanock, R. M., Kapikian, A. Z. and Parrott, R. H. 1983. Pediatric viral gastroenteritis during eight years of study. J. Clin. Microbiol. 10, 71-78.
  5. Browning, G. F., Fitzgerald, T. A., Chalmers, R. M. and Snodgrass, D. R. 1991. A novel group A rotavirus G serotype; serological and genomic characterization of equine isolate F123. J. Clin. Microbiol. 29, 2043-2046.
  6. Chung, J. K., Song, H. J., Kim, S. H., Seo, J. J., Kee, H. Y., Kim, E. S., Ha, D. R., Ryu, P. Y. and Lee, J. I. 2006. Epidemiological study of viral diarrhea in Gwangju area during 2000-2002. J. Bacteriol. Virol. 36, 195-203. https://doi.org/10.4167/jbv.2006.36.3.195
  7. Estes, M. K., Palmer, E. L. and Obijesk, J. F. 1983. Rotaviruses: a review. Curr. Top Microbiol. Immunol. 105, 123-184. https://doi.org/10.1007/978-3-642-69159-1_3
  8. Estes, M. K. and Cohen, J. 1989. Rotavirus gene structure and function. Microbiol. Rev. 53, 410-449.
  9. Han, N. S. 2007. Epidemiological study of viral gastroenteritis and genetic distribution of group A rotavirus strains isolated in Busan. Ph. D. Thesis, Pusan National University, Busan, Korea.
  10. Im, H. R., Jeon, I. S., Tchah, H., Im, J. S., Ryoo, E., Sun, Y. H., Cho, K. H., Im, H. J., Lee, G. H., Lee, H. S., Kang, Y. J. and Noh, Y. G. 2003. Influence of climate factors and PM10 on rotaviral infection: a seasonal variation study. Korea J. Pediatr. Gastroenterol. Nutr. 6, 120-128.
  11. Jain, V., Das, B. K., Bhan, M. K., Glass, R. I. and Gentsch, J. R. 2001. Great diversity of group A rotavirus strains and high prevalence of mixed rotavirus infections in India. J. Clin. Microbiol. 39, 3524-3529. https://doi.org/10.1128/JCM.39.10.3524-3529.2001
  12. Kang, J. O., Kilgore, P., Kim, J. S., Nyambat, B., Kim, J. G., Suh, H. S., Yoon, Y. M., Jang, S. J., Chang, C. H., Choi, S. W., Kim, M. N., Gentsch, J., Bresee, J. and Glass, R. 2005. Molecular epidemiological profile of rotavirus in South Korea, July 2002 through June 2003: Emergence of G4P[6] and G9P[8] strains. J. Infect .Dis. 192, 57-63.
  13. Kapikian, A. Z., Hoshino, Y. and Chanock, R. M. 2001. Rotaviruses. pp.1787-1833, 4th eds. Fields Virology. Lippincott Williams & Wilkins, Philadelphia.
  14. Kirkwood, C. D. and Palombo, E. A. 1997. Genetic characterization of the rotavirus nonstructural protein, NSP4. Virology 236, 258-265. https://doi.org/10.1006/viro.1997.8727
  15. Konno, T., Suzuki, H., Katsushima, N., Imai, A., Tazawa, F., Kutsuzawa, T., Kitaoka, S., Sakamoto, M., Yazaki, N. and Ishida, N. 1983. Influence of temperature and relative humidity on human rotavirus infection in Japan. J. Infect. Dis. 147, 125-128. https://doi.org/10.1093/infdis/147.1.125
  16. Korea meteorological administration, Annual Climatological reports. http://www. Kma.go.kr.
  17. Lee, J. M., Kim, H. Y., Lee, M. Y., Lee, K. B., Cheon, D. S. and Lee, Y. M. 2007. The prevalence and genotypic distribution of group A rotavirus detected from patients with acute gastroenteritis patients in Incheon. J. Bacteriol. Virol. 37, 39-45. https://doi.org/10.4167/jbv.2007.37.1.39
  18. Lee, K. B., Kim, J. W., Lee, S. W., Cheon, D. S., Jee, Y. M. and Kang, S. Y. 2007. Isolation and Characterization of G9 Human rotaviruses. J. Bacteriol. Virol .37, 231-240. https://doi.org/10.4167/jbv.2007.37.4.231
  19. Moe, K. and Shirley, J. A. 1982. The effects of relative humidity and temperature on the survival of human rotavirus in faeces. Arch. Virol. 72, 179-186. https://doi.org/10.1007/BF01348963
  20. Parashar, U. D., Hummelman, E. G., Bresee, J. S., Miller, M. A. and Glass, R. I. 2003. Global illness and deaths caused by rotavirus disease in children. Emerg. Infect. Dis. 9, 565-572. https://doi.org/10.3201/eid0905.020562
  21. Park, S. K. 2004. Etiological study on the patients with diarrhea in Daejon region and genetic analysis of rotavirus isolates. Ph. D. Thesis, Chungnam National University, Daejon, Korea.
  22. Park, S. K., Heo, Y. S., Bing, S. H., Cheon, D. S., Chu, J. G. and Jun, M. H. 2007. Epidemiological surveillance of P and G genotypes of group A rotavirus. J. Bacteriol. Virol. 37, 169-175. https://doi.org/10.4167/jbv.2007.37.3.169
  23. Sato, T., Suzuki, H., Kitaoka, S., Konnon, T. and Ishida, N. 1986. Patterns of polypeptide synthesis in human rotavirus infected cell. Arch. Virol. 90, 29-40. https://doi.org/10.1007/BF01314142
  24. Seo, J. K. and Sim, J. G. 2000. Overview of rotavirus infection in Korea. Pediatr. Int. 42, 406-410. https://doi.org/10.1046/j.1442-200x.2000.01250.x
  25. Yeager, M., Dryden, K. A., Olson, N. H., Greenberg, H. B. and. Baker, T. S. 1990. Three-dimensional structure of rhesus rotavirus by cryoelectron microscopy and image reconstruction. J. Clin. Mirobiol. 110, 2133-2144.

피인용 문헌

  1. The Molecular Epidemiology of Circulating Group A Rotavirus in Gwangju Metropolitan City, Korea: 2008~2012 vol.44, pp.2, 2014, https://doi.org/10.4167/jbv.2014.44.2.197
  2. Molecular Characterization of Norovirus and Rotavirus in Outbreak of Acute Gastroenteritis in Seoul vol.43, pp.4, 2013, https://doi.org/10.4167/jbv.2013.43.4.307