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Physicochemical Properties and Antioxidative Activities of Mixed Citrus and Carrot Juice

감귤당근 혼합주스의 이화학적 특성 및 항산화효과

  • Oh, You-Sung (Biotechnology Regional innovation Center, Jeju National University) ;
  • Hwang, Joon-Ho (Biotechnology Regional innovation Center, Jeju National University) ;
  • Oh, Hyun-Joeng (Biotechnology Regional innovation Center, Jeju National University) ;
  • Lim, Sang-Bin (Biotechnology Regional innovation Center, Jeju National University)
  • 오유성 (제주대학교 생명과학기술혁신센터) ;
  • 황준호 (제주대학교 생명과학기술혁신센터) ;
  • 오현정 (제주대학교 생명과학기술혁신센터) ;
  • 임상빈 (제주대학교 생명과학기술혁신센터)
  • Received : 2011.10.07
  • Accepted : 2012.04.23
  • Published : 2012.05.31

Abstract

Four types of mixed citrus and carrot juice (CCJ) were prepared with citrus-pressed juice and cake, and carrot-pressed juice. Their physicochemical properties and antioxidative activities were investigated. The four types of juices were created using different ratios of citrus-pressed juice, carrot-pressed juice, citrus-pressed cake, and additives. The mixing ratios of the four CCJ were as follows (all ratios given in the order of citrus-pressed juice : carrot-pressed juice : citrus-pressed cake : additives; 70:30:0:0 for CCJ-1, 65:30:0:5 for CCJ-2, 65:30:5:0 for CCJ-3, and 60:30:10:0 for CCJ-4. Acidity was low in CCJ-3 and -4 at 0.82 and 0.80, respectively, compared with 0.95 in CCJ-1. The fructose, glucose, and sucrose content was 2.11~5.76 g/100 g, 1.20~2.75 g/100 g, and 3.00~4.21 g/100 g, respectively. Total phenolic content was 1.17 and 1.22 times high as 863 and 898 mg% in CCJ-3 and -4, respectively, compared with 735 mg% in CCJ-1. DPPH radical scavenging activities of methanol extracts of CCJ-3 and -4 were 3.05 and 3.29 times as high as 58.7% and 63.3%, respectively, compared with 19.2% in CCJ-1. Superoxide anion scavenging activities were also 1.67 and 1.80 times higher in CCJ-3 and -4 than that of CCJ-1. Inhibition of NO production in methanol extracts of CCJ-4 were 1.15 and 1.57 times as high as 20.9% and 28.5%, respectively, compared with 18.2% in CCJ-1. Based on the sensory evaluation, CCJ-3 was more preferable in terms of color, flavor, taste, and overall preference than CCJ-1, -2, and -4. It was concluded that CCJ-3 made with 65% citrus-pressed juice, 30% carrot-pressed juice, and 5% citrus-pressed cake were high in antioxidative activity and the inhibition of NO production, and more preferable in terms of sensory attributes.

제주산 친환경 감귤과 당근의 착즙액과 감귤착즙박을 이용하여 4개의 감귤당근 혼합주스 시제품을 제조한 후 이화학적 특성과 항산화 활성을 측정하였다. 각 시제품의 조성은 CCJ-1이 감귤착즙액 :당근착즙액 : 50% 감귤착즙박(착즙박 : 물=1:1) :올리고당 등 첨가물이 70:30:0:0, CCJ-2가 65: 30:0:5, CCJ-3이 65:30:5:0, CCJ-4가 60:30:10:0이었다. 산도는 감귤착즙박을 첨가한 시제품(CCJ-3, 4)이 각각 0.82와 0.80으로 첨가하지 않은 시제품(CCJ-1)의 0.95보다 낮았다. 유리당 함량은 fructose가 2.11~5.76 g/100 g, glucose가 1.20~2.75 g/100 g, sucrose가 3.00~4.21 g/100 g으로, 특히 fructose 함량에서 많이 차이를 나타내었다. 총 페놀 함량은 CCJ-3과 CCJ-4가 각각 863 mg%과 898 mg%로 CCJ-1(735 mg%)보다 각각 1.17배와 1.22배 높았다. DPPH radical 소거능은 2,500 ${\mu}g/mL$의 농도에서 CCJ-3과 CCJ-4가 각각 58.7%와 63.3%로 CCJ-1(19.2%)보다 각각 3.05배와 3.29배 높았다. Superoxide anion radical 소거활성도 CCJ-3과 CCJ-4가 625 ${\mu}g/mL$에서 CCJ-1보다 각각 1.67배와 1.80배 높았다. NO 생성 저해능은 CCJ-3과 CCJ-4가 각각 20.9%와 28.5%로 CCJ-1(18.2%)보다 각각 1.15배와 1.57배 높았다. 감귤당근 혼합주스에 대한 관능평가 결과 시제품 CCJ-3은 색상, 향, 맛 그리고 전반적인 선호도가 4개의 시제품 중에서 상대적으로 가장 높은 것으로 나타났다. 결론적으로 감귤착 즙액 65%에 당근 착즙액 30%와 50% 감귤착즙박을 5% 첨가한 시제품 CCJ-3이 비교적 높은 항산화 활성과 항염증 활성을 나타내었고 선호도가 상대적으로 가장 높게 나타났다.

Keywords

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