• Korean Journal of Food Science and Technology
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• v.35 no.6
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• pp.1104-1109
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• 2003

Response surface methodology (RSM) was used to investigate the effects of the processing parameters on supercritical $CO_2\;(SC-CO_2)$. extraction of total carotenoids and ${\beta}$-cyptoxanthin from Citrus unshiu press cake. The parameters tested were $SC-CO_2$ pressure, dynamic extraction time, and concentration of ethanol added as the modifier to $CO_2$. Experimental data correlated well with the processing parameters (p<0.01), and there was a high statistically significant multiple regression relationship for the extraction of total carotenoids and ${\beta}-cyrptoxanthin$ ($R^2=0.9789$ and 0.9796, respectively). The optimal processing conditions were extraction pressure 33.4 and 37.3 MPa, extraction time 39.6 and 41.0 min, ethanol concentration 18.6 and 17.0% for total carotenoids and ${\beta}-cryptozanthin$, respectively. Maximum extraction yields predicted by RSM were 61.1 and 95.8% ppm, respectively. The extraction yield of total carotenoids increased asymptotically with the increase of the extraction pressure. It increased in proportion to extraction time and concentration of the cosolvent. The extraction yield of ${\beta}-cryptoxanthin$ increased with extraction pressure, extraction time, and concentration of the cosolvent. The extraction time and the concentration of the cosolvent, and the interaction between extraction time and the concentration of the cosolvent significantly affected the extraction yields of carotenoids from C. unshiu press cake.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.5
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• pp.598-604
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• 2012

Four types of mixed citrus and carrot juice (CCJ) were prepared with citrus-pressed juice and cake, and carrot-pressed juice. Their physicochemical properties and antioxidative activities were investigated. The four types of juices were created using different ratios of citrus-pressed juice, carrot-pressed juice, citrus-pressed cake, and additives. The mixing ratios of the four CCJ were as follows (all ratios given in the order of citrus-pressed juice : carrot-pressed juice : citrus-pressed cake : additives; 70:30:0:0 for CCJ-1, 65:30:0:5 for CCJ-2, 65:30:5:0 for CCJ-3, and 60:30:10:0 for CCJ-4. Acidity was low in CCJ-3 and -4 at 0.82 and 0.80, respectively, compared with 0.95 in CCJ-1. The fructose, glucose, and sucrose content was 2.11~5.76 g/100 g, 1.20~2.75 g/100 g, and 3.00~4.21 g/100 g, respectively. Total phenolic content was 1.17 and 1.22 times high as 863 and 898 mg% in CCJ-3 and -4, respectively, compared with 735 mg% in CCJ-1. DPPH radical scavenging activities of methanol extracts of CCJ-3 and -4 were 3.05 and 3.29 times as high as 58.7% and 63.3%, respectively, compared with 19.2% in CCJ-1. Superoxide anion scavenging activities were also 1.67 and 1.80 times higher in CCJ-3 and -4 than that of CCJ-1. Inhibition of NO production in methanol extracts of CCJ-4 were 1.15 and 1.57 times as high as 20.9% and 28.5%, respectively, compared with 18.2% in CCJ-1. Based on the sensory evaluation, CCJ-3 was more preferable in terms of color, flavor, taste, and overall preference than CCJ-1, -2, and -4. It was concluded that CCJ-3 made with 65% citrus-pressed juice, 30% carrot-pressed juice, and 5% citrus-pressed cake were high in antioxidative activity and the inhibition of NO production, and more preferable in terms of sensory attributes.

• Nutrition Research and Practice
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• v.5 no.5
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• pp.389-395
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• 2011

In the present study, a suitable drying method was developed for citrus press cakes (CPCs), which are produced as a by-product in citrus juice plants, and the protective effect of methanol extract of CPCs prepared by far-infrared radiation (FIR) drying against $H_2O_2$-induced DNA damage was evaluated versus that of freeze-dried CPCs. Methanol extract of FIR-dried CPCs exhibited comparatively good ROS scavenging activity versus the freeze-dried CPCs at the concentration of 100 ${\mu}g$/mL. The extract strongly enhanced the cell viability against $H_2O_2$-induced oxidative damage in Vero cells. Lipid peroxidation inhibitory activity of the extract from FIR-dried CPCs was comparable to that of the extract from freeze-dried CPCs. This sample also exhibited good protective effects against $H_2O_2$-mediated cell apoptosis as demonstrated by decreased apoptotic body formation in the nuclear staining with Hoechst 33342. In the comet assay, the CPC extracts exhibited strong inhibitory effects against $H_2O_2$-mediated DNA damage in a dose-dependent manner. Thus, this study demonstrated that FIR drying effectively preserves CPC as a functionally important natural antioxidant source and the FIR drying can be adapted for drying CPCs and is more economical for massive production than freeze drying.