Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Red-Koji Fermented Scutellariae Radix Extracts on Lipopolysaccharide-induced Rat Acute Lung Injury

홍국발효 황금이 Lipopolysaccharide 유발 급성 폐손상에 미치는 영향

  • Kim, Koang Lok (Department of Internal Medicine, College of Oriental Medicine, Dongeui University) ;
  • Kwon, Kyoung Man (Department of Internal Medicine, College of Oriental Medicine, Dongeui University) ;
  • Yun, Yong Jae (Department of Internal Medicine, College of Oriental Medicine, Dongeui University) ;
  • Lee, Young Jun (Department of Internal Medicine, College of Oriental Medicine, Dongeui University) ;
  • Park, Dong Il (Department of Internal Medicine, College of Oriental Medicine, Dongeui University) ;
  • Kim, Jong Dae (Department of Internal Medicine, College of Oriental Medicine, Deagu Hanny University) ;
  • Jung, Tae Young (Department of Diagnostics, College of Oriental Medicine, Deagu Hanny University)
  • 김광록 (동의대학교 한의과대학 내과학교실) ;
  • 권경만 (동의대학교 한의과대학 내과학교실) ;
  • 윤용재 (동의대학교 한의과대학 내과학교실) ;
  • 이영준 (동의대학교 한의과대학 내과학교실) ;
  • 박동일 (동의대학교 한의과대학 내과학교실) ;
  • 김종대 (대구한의대학교 한의과대학 내과학교실) ;
  • 정태영 (대구한의대학교 한의과대학 진단학교실)
  • Received : 2012.09.10
  • Accepted : 2012.10.21
  • Published : 2012.12.25
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Abstract

In the present study, the possibility of whether the pharmacological effects of Scutellariae Radix Aqueous Extracts(SR) were favorably changed by report that lipopolysaccharide(LPS)-induced rat acute lung injury was treated with Red-Koji(Monascus purpureus 12002) fermentation. Three different dosages of Red-Koji fermented SR extract(fSR), 125, 250 and 500 mg/kg were orally administered once a day for 28 days before LPS(Escherichia coli 0111:B4) treatments, and then 5 hours after LPS treatment(500 ${\mu}g$/head, intra trachea instillation), all rats were sacrificed. Changes in the body weights, lung weights, pulmonary transcapillary albumin transit, arterial gas parameters(pH, $PaO_2$ and $PaCO_2$) bronchoalveolar lavage fluid(BALF) protein, lactate dehydrogenase(LDH) and proinflammatory cytokine tumor necrosis factor-${\alpha}$(TNF-${\alpha}$), interleukin-$1{\beta}$(IL-$1{\beta}$) contents, total cell numbers, neutrophil and alveolar macrophage ratios, lung malondialdehyde(MDA), myeloperoxidase(MPO), proinflammatory cytokine TNF-${\alpha}$ and IL-$1{\beta}$ contents were observed with histopathology of the lung, changes on luminal surface of alveolus(LSA), thickness of alveolar septum, number of polymorphonuclear neutrophils(PMNs). As results of LPS-injection, dramatical increases in lung weights, pulmonary transcapillary albumin transit increases in $PaCO_2$, decreases in pH of arterial blood and $PaO_2$, increases of BALF protein, LDH, TNF-${\alpha}$ and IL-$1{\beta}$ contents, total cells, neutrophil and alveolar macrophage ratios, lung MDA, MPO, TNF-${\alpha}$ and IL-$1{\beta}$ contents increases were detected with decreases in LSA and increases of alveolar septum and PMNs numbers, respectively as compared with intact control. Especially fSR 125 mg/kg showed quite similar favorable effects on the LPS-induced acute lung injuries as compared with 60 mg/kg of ${\alpha}$-lipoic acid and 250 mg/kg of SR. The results suggest that over 125 mg/kg of fSR extracts showed favorable effects on the LPS-induced acute lung injury mediated by their antioxidant and anti-inflammatory effects. Moreover, increases of the pharmacological effects of SR on LPS-induced acute lung injury were observed by Red-Koji fermentation in this study, at least 2-fold higher.

Keywords

References

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