Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Protective Effects of Younggyechulgam-tang in Cisplatin-induced Ototoxicity

Cisplatin에 의한 이독성(耳毒性)에서 영계출감탕(苓桂朮甘湯)의 보호 효과

  • Jeon, Ho Seong (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Park, Rae Gil (Department of Microbiology & Vestibulocochlear Research Center, College of Medicine, Wonkwang University) ;
  • So, Hong Seob (Department of Microbiology & Vestibulocochlear Research Center, College of Medicine, Wonkwang University) ;
  • Chong, Myong Soo (Department of Preventive Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Su Kyung (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University)
  • 전호성 (원광대학교 한의학전문대학원) ;
  • 박래길 (원광대학교 의과대학 미생물학교실 및 전정와우기관 연구센터) ;
  • 소홍섭 (원광대학교 의과대학 미생물학교실 및 전정와우기관 연구센터) ;
  • 정명수 (원광대학교 한의과대학 예방의학교실) ;
  • 이수경 (원광대학교 한의과대학 한방재활의학과교실)
  • Received : 2012.08.21
  • Accepted : 2012.10.17
  • Published : 2012.10.25
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Abstract

The water extract of Younggyechulgam-tang (YGCGT) has been traditionally used in treatment of tinnitus in Oriental Medicine. However, little is known about the mechanism by which YGCGT rescues ototoxicity. The purpose of the present study is to investigate the protective effect of YGCGT against cisplatin-induced toxicity in HEI-OC1 auditory cells, organotypic cultures of cochlear explants from three-day postnatal rats (P3). Pretreatment with YGCGT ameliorated apoptotic death induced by cisplatin in HEI-OC1 cells and organotypic cultures of Corti's organ. YGCGT pretreatment also significantly suppressed cisplatin-induced increases in intracellular reactive oxygen species (ROS). Treatment with YGCGT resulted in an increased expression of HO-1 and Bcl-2. These results suggest that YGCGT induced HO-1 signaling pathway, which ultimately prevents free radical stresses from cisplatin and further contributes to protect auditory sensory hair cells from free radicals produced by cisplatin.

Keywords

Acknowledgement

Supported by : 원광대학교

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