• Journal of Physiology & Pathology in Korean Medicine
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• v.26 no.5
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• pp.699-706
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• 2012

The water extract of Younggyechulgam-tang (YGCGT) has been traditionally used in treatment of tinnitus in Oriental Medicine. However, little is known about the mechanism by which YGCGT rescues ototoxicity. The purpose of the present study is to investigate the protective effect of YGCGT against cisplatin-induced toxicity in HEI-OC1 auditory cells, organotypic cultures of cochlear explants from three-day postnatal rats (P3). Pretreatment with YGCGT ameliorated apoptotic death induced by cisplatin in HEI-OC1 cells and organotypic cultures of Corti's organ. YGCGT pretreatment also significantly suppressed cisplatin-induced increases in intracellular reactive oxygen species (ROS). Treatment with YGCGT resulted in an increased expression of HO-1 and Bcl-2. These results suggest that YGCGT induced HO-1 signaling pathway, which ultimately prevents free radical stresses from cisplatin and further contributes to protect auditory sensory hair cells from free radicals produced by cisplatin.

• Journal of Korean Medicine Rehabilitation
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• v.28 no.2
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• pp.1-20
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• 2018

Objectives This study was conducted to experimentally evaluate the effects of Younggyechulgam-tang-ga Hwanggi(YGT) on obesity in mice induced by high fat diet. Methods The experiment was conducted with 4-week-old male mice divided into 5 groups. They were a normal diet group(Nor), a high fat diet group(Veh), a positive drug control group-orlistat 40 mg/kg(Oris), a 1.08 g/kg group(YGTL), and a 2.16 g/kg group(YGTH), and were tested for five weeks. Changes in antioxidant activity, body weight, organ weight, ROS, AST, ALT, TC, TG, HDL-C, LDL-C and lipid metabolism protein were checked. Results YGTL and YGTH group significantly reduced body weight compared to Veh group. YGTH group significantly reduced visceral fat weights compared to Veh group. In blood biochemistry analysis, ROS, AST, ALT, TC, TG and LDL-C in YGTL and YGTH group were significantly lower than Veh group. HDL-C increased significance in YGTL and YGTH group. In antioxidation protein analysis, Catalase, GPx and HO-1 have increased significantly in YGTL and YGTH group. YGTH group have increased $PPAR-{\alpha}$, p-AMPK compared to Veh group. but decreased FAS. SREBP-1, p-ACC levels in YGTL and YGTH group were decreased compared to Veh group, however CPT-1, UCP-2 levels in YGTL and YGTH group were increased compared to Veh group. Conclusions YGT has anti-obesity effects by regulating lipolysis and antioxidation in a diet-induced obesity model. Additional clinical studies are needed.