Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Effect of UV or Ethanol Treatment on the Arcobacter butzleri Contaminated on Pork

돈육에 오염 된 Arcobacter butzleri에 대한 자외선 또는 에탄올 처리에 따른 효과

  • Lee, Min-Hwa (Department of Food and Nutrition, Chung-Ang University) ;
  • Choi, Chang-Sun (Department of Food and Nutrition, Chung-Ang University)
  • 이민화 (중앙대학교 자연과학대학 식품영양학과) ;
  • 최창순 (중앙대학교 자연과학대학 식품영양학과)
  • Received : 2011.08.31
  • Accepted : 2012.03.16
  • Published : 2012.04.30
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Abstract

Although Arcobacter butzleri is a foodborne emerging pathogen causing gastroenteritis in human and animals, there are a few researches on the physical and chemical control methods. The aim of this study was to investigate the effect of ultraviolet radiation or ethanol treatment on A. butzleri. To demonstrate the UV effect, 8 ${\log}_{10}CFU$/mL of A. butzleri were spiked on stainless steel and the pork was then exposed to 250 nm of ultraviolet light for 108-648 mWs/$cm^2$. To ascertain the effect of ethanol, A. butzleri and A. butzleri spiked pork were soaked or sprayed 10, 35 and 70% of ethanol for 10 to 30 min. A. butzleri significantly decreased all of the UV doses in stainless steel, whereas, the reduction was just $0.92{\pm}0.62-1.29{\pm}0.34\;{\log}_{10}CFU$/mL in pork spiked with A. butzleri. In the ethanol groups, A. butzleri decreased significantly in 35% or 70% of ethanol in contrast, the bacterial counts were dropped slightly in A. butzleri spiked pork groups. Therefore, it is necessary to develop various kinds of control methods or hurdle technology for A. butzleri.

본 연구에서는 Arcobacter spp. 중 사람에게 위장염을 가장 빈번하게 일으키며 전 세계적으로 식육에 가장 많이 오염되어 있는 A. butzleri에 대해 자외선과 에탄올의 살균 효과를 확인하고자 하였다. $3{\times}3cm^2$ 크기의 스테인리스스틸 표면과 돈육 샘플에 각각 8 ${\log}_{10}CFU$/mL의 A. butzleri를 $200{\mu}L$씩 접종한 후 254 nm 선량으로 108-648 mWs/$cm^2$의 자외선을 조사하였다. 또한 10, 35, 70%의 에탄올에 broth 상태의 A. butzleri와 A. butzleri를 접종한 돈육을 10-30분 간 침지시키거나 에어로졸 상태로 분무 시켰다. 스테인리스스틸 표면의 A. butzleri에 자외선 조사 시 A. butzleri는 모든 자외선 선량에서 유의적으로 감소하였으나 돈육에 오염된 A. butzleri는 유의적 차이가 없거나 감소율이 매우 적은 것으로 나타났다(최고 감소량 $0.92{\pm}0.62-1.29{\pm}0.34\;{\log}_{10}CFU$/mL). 또한 broth 상태의 A. butzleri에 에탄올을 처리했을 경우 모든 농도의 에탄올에서 A. butzleri가 유의적으로 감소하였으나 식육에 오염 된 A. butzleri는 유의적인 차이가 없거나 감소율이 매우 낮은 것으로 나타났다(10% 에탄올의 최고 감소량은 $0.16{\pm}0.42-0.77{\pm}0.65\;{\log}_{10}CFU$/mL, 35% 에탄올은 $0.59{\pm}0.38-1.52{\pm}0.60\;{\log}_{10}CFU$/mL, 70% 에탄올은 $1.33{\pm}0.43-2.83{\pm}0.52\;{\log}_{10}CFU$/mL).

Keywords

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