농업과학연구 (Korean Journal of Agricultural Science)

  • 제39권4호
  • /
  • Pages.467-471
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  • 2012
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  • 2466-2402(pISSN)
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  • 2466-2410(eISSN)
충남대학교 농업과학연구소 (Institute of Agricultural Science, CNU)
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Cellular system에서의 깻잎의 ONOO-에 의한 산화적 스트레스 개선 및 항노화 효과

The protective effect of Perilla frutescens from ONOO--induced oxidative stress and antiaging effect under cellular system

  • 김현영 (경남과학기술대학교 식품과학부) ;
  • 황보라 (부산대학교 식품영양학과 및 노인생활환경연구) ;
  • ;
  • 조은주 (부산대학교 식품영양학과 및 노인생활환경연구)
  • Kim, Hyun Young (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Hwang, Bo Ra (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University) ;
  • Wu, Ting Ting (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University)
  • 투고 : 2012.08.31
  • 심사 : 2012.11.08
  • 발행 : 2012.12.31
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초록

In this study, we investigated the antioxidative and antiaging activity of Perilla frutescens using LLC-$PK_1$ porcine renal epithelial cell and WI-38 human diploid fibroblast cell. The extract from Perilla frutescens showed strong protective effect against nitric oxide (NO) and superoxide ($O_2{^-}$)-induced oxidative stress generated by sodium nitroprusside (SNP) and pyrogallol, respectively. The result showed that P. frutescens increased the cell viability and showed scavenging activity of NO and $O_2{^-}$. In addition, the extract of P. frutescens exerted the protective effect against peroxynitrite ($ONOO^-$) induced by 3-morpholinosydnonimine. It suggests that P. frutescens would have the protective role against $ONOO^-$ itself and its precursors, NO and $O_2{^-}$. Furthermore, the aging model of hydrogen peroxide ($H_2O_2$)-treated WI-38 human diploid fibroblast was employed to investigate the anti-aging effect of P. frutescens. $H_2O_2$-treated WI-38 cells showed the loss of cell viability, however before-treatment with P. frutescens to WI-38 cells under premature senescence could delay the cellular aging process. The present study suggests the antioxidative and antiaging potential against free radical-induced oxidative damage of P. frutescens.

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참고문헌

  1. Andersen HR, Nielsen JB, Nielsen F, Grandjean P. 1997. Antioxidative enzyme activities in human erythrocytes. Clinical Chemistry 43: 562-568.
  2. Bokov A, Chauhuri A, Richardson A. 2004. The role of oxidative damage and stress in aging. Mechanisms of Ageing and Development. 125: 811-826. https://doi.org/10.1016/j.mad.2004.07.009
  3. Galla HJ. 1993. Nitric oxide, NO, an intracellular messenger. Angewandte Chemie International Edition 32: 378-380. https://doi.org/10.1002/anie.199303781
  4. Gibson GE, Huang HM. 2005. Oxidative stress in Alzheimer's disease. Neurobiology of Aging 26, 575-578. https://doi.org/10.1016/j.neurobiolaging.2004.07.017
  5. Han HS, Park JH, Choe HJ, Son JH, Kim YH, Kim S and Choe C. 2004. Biochemical analysis and physiological activity of perilla leaves. Journal of the Korean Society of Food Culture 19: 94-105.
  6. Kuhn M. Oxygen free radicals and antioxidants: An overview of now how antioxidants protect the body from disease. 2003. American Journal of Nursing 103: 58-62.
  7. Lee KI, Rhee SH, Kim JO, Chung HY and Park KY. 1993. Antimutagenic and antioxidative effects of perilla leaf extracts. Journal of the Korean Society of Food Science and Nutrition 22: 175-180.
  8. Lim DG. 2004. Oxidative stress; reactive oxygen species and nitric oxide. Korean Journal of Critical Care Medicine 19: 2.
  9. Makino T, Furuta Y, Wakushima H, Fujii H, Saito K and Kano Y. 2003. Anti-allergic effect of Perilla frutescens and it's active constituents. Phytotherapy Research 17: 240-243. https://doi.org/10.1002/ptr.1115
  10. MeCartney-Francis N, Allen JB, Mizel DE, Albina JE, Xie QW, Nathan CF and Wahl SM. 1993. Suppression of arthritis by an inhibitor of nitric oxide synthase. The Journal of Experimental Medicine 178: 749-754. https://doi.org/10.1084/jem.178.2.749
  11. Mosmann T. 1983. Rapid colormetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65: 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  12. Nunokawa Y, Tanaka S. 1992. Interferon-$\gamma$inhibits proliferation of rat vascular smooth muscle cells by nitric oxide generation. Biochemical and Biophysical Research Communications 188: 409-415. https://doi.org/10.1016/0006-291X(92)92400-R
  13. Nuttall SL, Kendall MJ, Martin U. 1999. Antioxidant therapy for the prevention of cardiovascular disease. QJM : Monthly Journal of the Association of Physicians 92: 239-244. https://doi.org/10.1093/qjmed/92.5.239
  14. Patel RP, McAndrew J, Sellak H, White CR, Jo H, Freeman BA, Darley-Usmar VM. 1999. Biological aspects of reactive nitrogen species. Biochimica et Biophysica Acta 1411: 385-400. https://doi.org/10.1016/S0005-2728(99)00028-6
  15. Peng J, Jones GL, Watson K. 2000. Stress proteins as biomakers of oxidative stress: effects of antioxidant supplements. Free Radical Biology and Medicine 28: 1598-1606. https://doi.org/10.1016/S0891-5849(00)00276-8
  16. Radi R, Beckman JS, Bush KM and Freeman BA. 1991. Peroxynitrite-induced membrane lipid peroxidation: the cytotoxic potential of superoxide and nitric oxide. Archives of Biochemistry and Biophysics 288: 481-487. https://doi.org/10.1016/0003-9861(91)90224-7
  17. Rath E, Haller D. 2011. Inflammation and cellular stress: a mechanistic link between immune-mediated and metabolically driven pathologies. European Journal of Nutrition 50: 219-233. https://doi.org/10.1007/s00394-011-0197-0
  18. Rubbo H, Darley-Usmar V and Freeman BA. 1996. Nitric oxide regulation of tissue free radical injury. Chemical Research in Toxicology 9: 809-820. https://doi.org/10.1021/tx960037q
  19. Ueda H, Yamazaki C and Yamazaki M. 2002. Luteolin as an antiinflammatory and anti-allergic constituent of Perilla frutescens. Biological & Pharmaceutical Bulletin 25: 1197-1202. https://doi.org/10.1248/bpb.25.1197
  20. Ueda H, Yamazaki C and Yamazaki M. 2003. Inhibitory effect of Perilla leaf extract and luteolin on mouse skin tumor promotion. Biological & Pharmaceutical Bulletin 26: 560-563. https://doi.org/10.1248/bpb.26.560
  21. Warnholtz A, Munzel T. 2000. Why do antioxidants fail to provide clinical benefit?. Current Controlled Trials in Cardiovascular Medicine 1: 38-40. https://doi.org/10.1186/CVM-1-1-038
  22. Yamazaki M and Saito K. 2006. Isolation and characterization of anthocyanin 5-O-glucosyltransferase in Perilla frutescens var. crispa by differential display. Methods in Molecular Biology 317: 255-266.
  23. Zupko I, Hohmann J, Redei D, Falkay G, Janicsak G and Mathe I. 2001. Antioxidant activity of leaves of salvia species in enzyme-dependent and enzyme-independent system of lipid peroxidation and their phenolic constituents. Planta Medica 67: 366-368. https://doi.org/10.1055/s-2001-14327

피인용 문헌

  1. Free Radical Scavenging Effect and Oxidative Stress Protective Activity of Domestic Processed Polygoni Multiflori Radix vol.44, pp.6, 2015, https://doi.org/10.3746/jkfn.2015.44.6.809