The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Genetic Diversity of Penicillium isolates Isolated from Pears with Postharvest Decay in Storage

수확 후 저장 배에서 분리한 Penicillium속 균의 유전적 다양성

  • Han, Do-Suk (Graduate School of Bio.&Information Technology, Hankyong National University) ;
  • Hong, Sung-Kee (National Academy of Agricultural Science, RDA) ;
  • Kang, Hee-Wan (Graduate School of Bio.&Information Technology, Hankyong National University)
  • 한도숙 (한경대학교 바이오정보기술대학원) ;
  • 홍성기 (국립농업과학원) ;
  • 강희완 (한경대학교 바이오정보기술대학원)
  • Received : 2012.03.15
  • Accepted : 2012.03.25
  • Published : 2012.04.01
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Abstract

This study was carried out to identify the genetic diversity of Penicillium isolates that were isolated from pears with postharvest decay in storage. URP-PCR was used to detect DNA diversity of 84 Penicillium isolates. Based on URP-PCR profiles, 18 Penicillium isolates were selected and their PCR polymorphic bands were produced by additional primers URP1F, URP2R, URP2F, and URP4R. UPGMA cluster analysis using the polymorphic bands showed four clustered groups and futhermore cultural and morphological features characterized the 18 Penicillium isolates. Group 1 was dominant, which occupies 70% in the four clustered groups and identified as P. expansum based on ITS sequence and morphological features.

평택일원의 6개의 배 저장고로부터 300개의 부패된 배를 수집 하여 병반부위로부터 곰팡이를이분리 하여 조사한 결과 Penicillium 균이 이병배중에서 84%가 분리되었다. 84 Penicillium 균주를 대상으로 URP2R primer로 균주 간의 DNA 다양성을 조사한결과 18 type의 Penicillium 균주를 검출 할 수 있었으며 URP1F, URP2F, URP2R, URP4R의 PCR 다형성밴드를 이용한 유전적 유연관계분석으로 4 group으로 나눌 수 있었다. 18 Penicillium균주의 형태적특징을 조사한결과 3가지의 특징적인 배양적 특징을 갖는 것으로 확인 되었으며 포자형성과 포자형태에서도 3가지 type이 관찰 되었는데 SP-15균주 type, KP-1-1 균주 type, SP-17 type으로 PCR 다형성결과와 유사한 경향을 나타내었다. SP-15형은 형태적, rDNA의 ITS 염기서열 분석에서 P. expansum로 동정 되었으며 분리된 Penicillium 균주 중 70%를 차지하는 우점종인 것으로 나타났으므로 향 후 URP-PCR profile은 종간, 종내 계통 분류에 유용하게 이용 할 수 있다.

Keywords

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