Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Effects of Preconceptional Ethanol Consumption on ADHD-Like Symptoms in Sprague-Dawley Rat Offsprings

  • Choi, In-Ah (Center for Neuroscience Research, SMART IBST and Department of Pharmacology, School of Medicine, Konkuk University) ;
  • Kim, Pitna (Center for Neuroscience Research, SMART IBST and Department of Pharmacology, School of Medicine, Konkuk University) ;
  • Joo, So-Hyun (Center for Neuroscience Research, SMART IBST and Department of Pharmacology, School of Medicine, Konkuk University) ;
  • Kim, Min-Kyeong (Center for Neuroscience Research, SMART IBST and Department of Pharmacology, School of Medicine, Konkuk University) ;
  • Park, Jin-Hee (Center for Neuroscience Research, SMART IBST and Department of Pharmacology, School of Medicine, Konkuk University) ;
  • Kim, Hee-Jin (College of Pharmacy and Uimyong Research Institute for Neuroscience, Samyook University) ;
  • Ryu, Jong-Hoon (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Cheong, Jae-Hoon (College of Pharmacy and Uimyong Research Institute for Neuroscience, Samyook University) ;
  • Shin, Chan-Young (Center for Neuroscience Research, SMART IBST and Department of Pharmacology, School of Medicine, Konkuk University)
  • Received : 2011.09.08
  • Accepted : 2011.12.19
  • Published : 2012.03.31
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Abstract

Ethanol exposure during gestational period is related to growth retardation, morphological abnormality, and even in neurological abnormalities including attention deficit/hyperactivity disorder (ADHD)-like behaviors on offspring. However, relatively little is known about the effects of maternal ethanol consumption prior to conception on their offspring. In this study, we investigated whether maternal ethanol administration during preconceptional phase produces ADHD-like behaviors in the rat offspring. Sprague-Dawley (SD) female rats were administrated ethanol via intragastric intubation with dosing regimen of 6 g/kg daily for 10 consecutive days and treated female rats then mated with non-treated male SD rats after 8 weeks. Another group subjected to the same procedure as those conducted on ethanol treated group except the saline administration instead of ethanol. Offspring was tested for their ADHD-like behaviors using open field test, Y maze test and impulsivity test that is performed in the aversive electronic foot shock paradigm. Offspring of preconceptional ethanol treated (EtOH) group showed hyperlocomotive activity, attention deficit and impulsivity. And reduction of striatal dopamine transporter (DAT) level was observed by Western blot in the EtOH group, compared to control (Con) group, while the immunohistochemical analysis exhibited increased expression of norepinephrine transporter (NET) in the frontal cortex. These results suggest that maternal ethanol consumption in the preconceptional phase induces ADHD-like behaviors in offspring that might be related to the abnormal expression of DAT and NET in rat.

Keywords

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