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http://dx.doi.org/10.4062/biomolther.2012.20.2.226

Effects of Preconceptional Ethanol Consumption on ADHD-Like Symptoms in Sprague-Dawley Rat Offsprings  

Choi, In-Ah (Center for Neuroscience Research, SMART IBST and Department of Pharmacology, School of Medicine, Konkuk University)
Kim, Pitna (Center for Neuroscience Research, SMART IBST and Department of Pharmacology, School of Medicine, Konkuk University)
Joo, So-Hyun (Center for Neuroscience Research, SMART IBST and Department of Pharmacology, School of Medicine, Konkuk University)
Kim, Min-Kyeong (Center for Neuroscience Research, SMART IBST and Department of Pharmacology, School of Medicine, Konkuk University)
Park, Jin-Hee (Center for Neuroscience Research, SMART IBST and Department of Pharmacology, School of Medicine, Konkuk University)
Kim, Hee-Jin (College of Pharmacy and Uimyong Research Institute for Neuroscience, Samyook University)
Ryu, Jong-Hoon (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University)
Cheong, Jae-Hoon (College of Pharmacy and Uimyong Research Institute for Neuroscience, Samyook University)
Shin, Chan-Young (Center for Neuroscience Research, SMART IBST and Department of Pharmacology, School of Medicine, Konkuk University)
Publication Information
Biomolecules & Therapeutics / v.20, no.2, 2012 , pp. 226-233 More about this Journal
Abstract
Ethanol exposure during gestational period is related to growth retardation, morphological abnormality, and even in neurological abnormalities including attention deficit/hyperactivity disorder (ADHD)-like behaviors on offspring. However, relatively little is known about the effects of maternal ethanol consumption prior to conception on their offspring. In this study, we investigated whether maternal ethanol administration during preconceptional phase produces ADHD-like behaviors in the rat offspring. Sprague-Dawley (SD) female rats were administrated ethanol via intragastric intubation with dosing regimen of 6 g/kg daily for 10 consecutive days and treated female rats then mated with non-treated male SD rats after 8 weeks. Another group subjected to the same procedure as those conducted on ethanol treated group except the saline administration instead of ethanol. Offspring was tested for their ADHD-like behaviors using open field test, Y maze test and impulsivity test that is performed in the aversive electronic foot shock paradigm. Offspring of preconceptional ethanol treated (EtOH) group showed hyperlocomotive activity, attention deficit and impulsivity. And reduction of striatal dopamine transporter (DAT) level was observed by Western blot in the EtOH group, compared to control (Con) group, while the immunohistochemical analysis exhibited increased expression of norepinephrine transporter (NET) in the frontal cortex. These results suggest that maternal ethanol consumption in the preconceptional phase induces ADHD-like behaviors in offspring that might be related to the abnormal expression of DAT and NET in rat.
Keywords
ADHD; Preconceptional ethanol consumption; Norepinephrine transporter; Dopamine transporter;
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1 Froehlich, T. E., Lanphear, B. P., Epstein, J. N., Barbaresi, W. J., Katusic, S. K. and Kahn, R. S. (2007) Prevalence, recognition, and treatment of attention-defi cit/hyperactivity disorder in a national sample of US children. Arch. Pediatr. Adolesc. Med. 161, 857-864.   DOI   ScienceOn
2 Gainetdinov, R. R. and Caron, M. G. (2003) Monoamine transporters: from genes to behavior. Annu. Rev. Pharmacol. Toxicol. 43, 261- 284.   DOI   ScienceOn
3 Garro, A. J., McBeth, D. L., Lima, V. and Lieber, C. S. (1991) Ethanol consumption inhibits fetal DNA methylation in mice: implications for the fetal alcohol syndrome. Alcohol Clin. Exp. Res. 15, 395-398.   DOI   ScienceOn
4 Giros, B., Jaber, M., Jones, S. R., Wightman, R. M. and Caron, M. G. (1996) Hyperlocomotion and indifference to cocaine and amphetamine in mice lacking the dopamine transporter. Nature 379, 606-612.   DOI   ScienceOn
5 Hausknecht, K. A., Acheson, A., Farrar, A. M., Kieres, A. K., Shen, R. Y., Richards, J. B. and Sabol, K. E. (2005) Prenatal alcohol exposure causes attention defi cits in male rats. Behav. Neurosci. 119, 302-310.   DOI
6 Haycock, P. C. (2009) Fetal alcohol spectrum disorders: the epigenetic perspective. Biol. Reprod. 81, 607-617.   DOI   ScienceOn
7 Huggins, J. E., Grant, T. and O'Malley, K. (2008) Suicide attempts among adults with fetal alcohol spectrum disorders: Clinical considerations. Ment. Health Aspects. Dev. Disabil. April-June, 33-41.
8 Hughes, R. N. (2004) The value of spontaneous alternation behavior (SAB) as a test of retention in pharmacological investigations of memory. Neurosci. Biobehav. Rev. 28, 497-505.   DOI   ScienceOn
9 Kaufman, M. H. (1997) The teratogenic effects of alcohol following exposure during pregnancy, and its infl uence on the chromosome constitution of the pre-ovulatory egg. Alcohol 32, 113-128.
10 Kessler, R. C., Berglund, P., Demler, O., Jin, R., Merikangas, K. R. and Walters, E. E. (2005) Lifetime prevalence and age-of-onset distributions of DSM-IV disorders in the National Comorbidity Survey Replication. Arch. Gen. Psychiatry 62, 593-602.   DOI   ScienceOn
11 Kim, J. S. and Shukla, S. D. (2006) Acute in vivo effect of ethanol (binge drinking) on histone H3 modifi cations in rat tissues. Alcohol 41, 126-132.
12 Knardahl, S. and Sagvolden, T. (1979) Open-field behavior of spontaneously hypertensive rats. Behav. Neural. Biol. 27, 187-200.   DOI   ScienceOn
13 Knopik, V. S., Jacob, T., Haber, J. R., Swenson, L. P. and Howell, D. N. (2009) Paternal alcoholism and offspring ADHD problems: a children of twins design. Twin. Res. Hum. Genet. 12, 53-62.   DOI
14 Knopik, V. S., Sparrow, E. P., Madden, P. A., Bucholz, K. K., Hudziak, J. J., Reich, W., Slutske, W. S., Grant, J. D., McLaughlin, T. L., Todorov, A., Todd, R. D. and Heath, A. C. (2005) Contributions of parental alcoholism, prenatal substance exposure, and genetic transmission to child ADHD risk: a female twin study. Psychol. Med. 35, 625-635.   DOI   ScienceOn
15 Livy, D. J., Maier, S. E. and West, J. R. (2004) Long-term alcohol exposure prior to conception results in lower fetal body weights. Birth. Defects. Res. B. Dev. Reprod. Toxicol. 71, 135-141.   DOI   ScienceOn
16 Lupton, C., Burd, L. and Harwood, R. (2004) Cost of fetal alcohol spectrum disorders. Am. J. Med. Genet. C. Semin. Med. Genet. 127C, 42-50.   DOI
17 Moeller, F. G., Barratt, E. S., Dougherty, D. M., Schmitz, J. M. and Swann, A. C. (2001) Psychiatric aspects of impulsivity. Am. J. Psychiatry 158, 1783-1793.   DOI   ScienceOn
18 Riley, E. P. and McGee, C. L. (2005) Fetal alcohol spectrum disorders: an overview with emphasis on changes in brain and behavior. Exp. Biol. Med (Maywood). 230, 357-365.
19 O'Leary, C. M., Nassar, N., Zubrick, S. R., Kurinczuk, J. J., Stanley, F. and Bower, C. (2010) Evidence of a complex association between dose, pattern and timing of prenatal alcohol exposure and child behaviour problems. Addiction 105, 74-86.   DOI   ScienceOn
20 Murillo-Fuentes, M. L., Artillo, R., Ubeda, N., Varela-Moreiras, G., Murillo, M. L. and Carreras, O. (2005) Hepatic S-adenosylmethionine after maternal alcohol exposure on offspring rats. Addict. Biol. 10, 139-144.   DOI   ScienceOn
21 Polanczyk, G., de Lima, M. S., Horta, B. L., Biederman, J. and Rohde, L. A. (2007) The worldwide prevalence of ADHD: a systematic review and metaregression analysis. Am. J. Psychiatry 164, 942-948.   DOI   ScienceOn
22 Roeleveld, N., Vingerhoets, E., Zielhuis, G. A. and Gabreëls, F. (1992) Mental retardation associated with parental smoking and alcohol consumption before, during, and after pregnancy. Prev. Med. 21, 110-119.   DOI   ScienceOn
23 Brown, R. T., Coles, C. D., Smith, I. E., Platzman, K. A., Silverstein, J., Erickson, S. and Falek, A. (1991) Effects of prenatal alcohol exposure at school age. II. Attention and behavior. Neurotoxicol. Teratol. 13, 369-376.   DOI   ScienceOn
24 Abel, E. L. (1994) Effects of physostigmine on male offspring sired by alcohol-treated fathers. Alcohol. Clin. Exp. Res. 18, 648-652.   DOI   ScienceOn
25 American Psychiatric Association. (1994) Diagnostic and statistical manual of mental disorder. (4th Ed.) American Psychiatry Press., Washington.
26 Barkley, R. A. (1990) Attention-defi cit hyperactivity disorder: A handbook for diagnosis and treatment. Guilford Press., New York.
27 Barr, C. L., Kroft, J., Feng, Y., Wigg, K., Roberts, W., Malone, M., Ickowicz, A., Schachar, R., Tannock, R. and Kennedy, J. L. (2002) The norepinephrine transporter gene and attention-defi cit hyperactivity disorder. Am. J. Med. Genet. 114, 255-259.   DOI   ScienceOn
28 Biederman, J., Kwon, A., Aleardi, M., Chouinard, V. A., Marino, T., Cole, H., Mick, E. and Faraone, S. V. (2005) Absence of gender effects on attention defi cit hyperactivity disorder: fi ndings in nonreferred subjects. Am. J. Psychiatry 162, 1083-1089.   DOI   ScienceOn
29 Brust, J. C. (2010) Ethanol and cognition: indirect effects, neurotoxicity and neuroprotection: a review. Int. J. Environ. Res. Public. Health 7, 1540-1557.   DOI
30 Choi, S. W., Stickel, F., Baik, H. W., Kim, Y. I., Seitz, H. K. and Mason, J. B. (1999) Chronic alcohol consumption induces genomic but not p53-specifi c DNA hypomethylation in rat colon. J. Nutr. 129, 1945- 1950.
31 Davids, E., Zhang, K., Tarazi, F. I. and Baldessarini, R. J. (2003) Animal models of attention-defi cit hyperactivity disorder. Brain Res. Brain Res. Rev. 42, 1-21.   DOI
32 DuPaul, G. J., McGoey, K. E., Eckert, T. L. and VanBrakle, J. (2001) Preschool children with attention-defi cit/hyperactivity disorder: impairments in behavioral, social, and school functioning. J. Am. Acad. Child Adolesc. Psychiatry 40, 508-515.   DOI   ScienceOn
33 Fernández-Checa, J. C., Colell, A. and García-Ruiz, C. (2002) S-Adenosyl- L-methionine and mitochondrial reduced glutathione depletion in alcoholic liver disease. Alcohol 27, 179-183.   DOI   ScienceOn
34 Floyd, R. L., Decoufl é, P. and Hungerford, D. W. (1999) Alcohol use prior to pregnancy recognition. Am. J. Prev. Med. 17, 101-107.   DOI   ScienceOn