전기화학회지 (Journal of the Korean Electrochemical Society)

  • 제15권4호
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  • Pages.249-255
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  • 2012
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  • 1229-1935(pISSN)
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  • 2288-9000(eISSN)
한국전기화학회 (The Korean Electrochemical Society)
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크레아틴의 방해영향을 줄인 크레아티닌 바이오센서

A Creatinine Biosensor with Reduced Interference from Creatine

  • 구현우 (광운대학교 자연과학대학 화학과) ;
  • 권기학 (광운대학교 자연과학대학 화학과) ;
  • 임은혜 (광운대학교 자연과학대학 화학과) ;
  • 신재호 (광운대학교 자연과학대학 화학과)
  • Gu, Hyunwoo (Department of Chemistry, Kwangwoon University) ;
  • Gwon, Kihak (Department of Chemistry, Kwangwoon University) ;
  • Lim, Eunhye (Department of Chemistry, Kwangwoon University) ;
  • Shin, Jae Ho (Department of Chemistry, Kwangwoon University)
  • 투고 : 2012.11.23
  • 심사 : 2012.11.28
  • 발행 : 2012.11.30
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초록

크레아티닌 센서의 생체시료 측정 시 가장 심각한 방해 작용을 발생하는 물질인 크레아틴을 효과적으로 제거하기 위하여 creatine kinase와 adenosine triphosphate를 사용한 두 번째 효소층을 도입하여 크레아틴에 대한 방해작용을 현저히 감소시켰다. 또한 평면형 소형 크레아티닌 센서를 개발하기 위해 탄소전극 표면에 Pt black(Pt-B)을 도입하여 표면적을 증가시킴으로써 전기화학적 감응 특성을 증가시킨 스크린 프린팅 방식의 Pt-B/C 전극을 제작하였다. 최적화된 소형 크레아티닌 센서를 흐름계 카트리지에 장착하여 미지시료를 측정한 결과 5% 이내의 오차 범위 내에서 우수한 측정 정확성과 재현성을 보임을 확인하였다.

The planar-type amperometric creatinine biosensor employing an additional enzyme layer containing creatine kinase and adenosine triphosphate was developed to eliminate severe interference from creatine. In the additional enzyme layer, an interfering substance, creatine is converted to noninterfering product, phosphocreatine. Furthermore, the carbon electrode electroplated with Pt black(Pt-B) was employed to fabricate creatinine biosensors with improved sensor performance(e.g., sensitivity, reliability, and reproducibility). The creatinine levels in an unknown sample were determined within less than 5% errors using creatinine microsensors equipped in a flow-cell cartridge.

키워드

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