미생물학회지 (Korean Journal of Microbiology)

  • 제48권4호
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  • Pages.246-253
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  • 2012
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
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인삼토양 내 근권세균의 다양성 및 식물에의 유용 특성

Molecular Diversity of Rhizobacteria in Ginseng Soil and Their Plant Benefiting Attributes

  • 홍은혜 (충남대학교 생명시스템과학대학 미생물.분자생명과학과) ;
  • 이선희 (충남대학교 생명시스템과학대학 미생물.분자생명과학과) ;
  • 알 타미즈 벤단 (인도 타밀나두 농업대학교) ;
  • 이영하 (충남대학교 생명시스템과학대학 미생물.분자생명과학과)
  • Hong, Eun Hye (Department of Microbiology & Molecular Biology, Chungnam National University) ;
  • Lee, Sun Hee (Department of Microbiology & Molecular Biology, Chungnam National University) ;
  • Vendan, Regupathy Thamizh (Agricultural College & Research Institute, Tamil Nadu Agricultural University) ;
  • Rhee, Young Ha (Department of Microbiology & Molecular Biology, Chungnam National University)
  • 투고 : 2012.10.02
  • 심사 : 2012.12.14
  • 발행 : 2012.12.31
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초록

연령을 달리하는 인삼토양 내 근권세균의 다양성과 이들 세균이 갖고 있는 식물생장에 유용한 특성에 대하여 조사하였다. 인삼 근권으로부터 총 15개 속에 포함되는 143 균주를 분리하였다. 인삼 연령에 따른 세균 군집의 변화가 있었으나, 분리 균주의 58%가 Firmicutes에 속하였다. 특히 Bacillus는 연령에 관계없이 가장 우점하는 속으로 나타났다. 이들 분리 균주 중 30개의 균주를 선발하여 세포벽 분해효소 분비, 인돌아세트산 생성, siderophore 생성, 인 가용화, 식물병원성 곰팡이의 저해능 등에 대하여 평가한 결과, 다수의 균주가 식물생장을 촉진할 수 있는 기능적 특성을 갖고 있었다. 이 중에서도 모든 조사 항목에 대하여 양성 반응을 보인 B. subtilis, B. amyloliquefaciens, B. velezensis, B. licheniformis 균주는 향후 인삼 경작을 위한 생장 촉진 미생물제제로 활용가치가 높을 것으로 기대된다.

The purpose of this study was to investigate the molecular diversity of rhizobacteria associated with ginseng of varying age levels and their plant benefiting attributes. A total of 143 different isolates belonging to 15 different bacterial genera were recovered. Although variation was found in the rhizobacterial community due to age of the plant, majority of bacteria belong to Firmicutes (58%). In which, Bacillus was found to be the predominant genus irrespective of age of the ginseng. To assess the plant benefiting attributes, 30 representative isolates were selected. The results indicated that some of the isolates could exhibit multiple plant growth promoting traits like secretion of cell wall degrading enzymes, production of indole-3-acetic acid, synthesis of siderophores, solubilization of phosphates and soil pathogens inhibition. It can be suggested that strains of B. subtilis, B. amyloliquefaciens, B. velezensis, and B. licheniformis were positive for all the above traits, which have potential to be used as plant growth promoting inoculants to improve ginseng crop in the future.

키워드

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  2. Bacterial Diversity and Community Structure in Korean Ginseng Field Soil Are Shifted by Cultivation Time vol.11, pp.5, 2016, https://doi.org/10.1371/journal.pone.0155055
  3. Comparative study of rhizobacterial communities in pepper greenhouses and examination of the effects of salt accumulation under different cropping systems vol.199, pp.2, 2017, https://doi.org/10.1007/s00203-016-1304-7