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http://dx.doi.org/10.7845/kjm.2012.047

Molecular Diversity of Rhizobacteria in Ginseng Soil and Their Plant Benefiting Attributes  

Hong, Eun Hye (Department of Microbiology & Molecular Biology, Chungnam National University)
Lee, Sun Hee (Department of Microbiology & Molecular Biology, Chungnam National University)
Vendan, Regupathy Thamizh (Agricultural College & Research Institute, Tamil Nadu Agricultural University)
Rhee, Young Ha (Department of Microbiology & Molecular Biology, Chungnam National University)
Publication Information
Korean Journal of Microbiology / v.48, no.4, 2012 , pp. 246-253 More about this Journal
Abstract
The purpose of this study was to investigate the molecular diversity of rhizobacteria associated with ginseng of varying age levels and their plant benefiting attributes. A total of 143 different isolates belonging to 15 different bacterial genera were recovered. Although variation was found in the rhizobacterial community due to age of the plant, majority of bacteria belong to Firmicutes (58%). In which, Bacillus was found to be the predominant genus irrespective of age of the ginseng. To assess the plant benefiting attributes, 30 representative isolates were selected. The results indicated that some of the isolates could exhibit multiple plant growth promoting traits like secretion of cell wall degrading enzymes, production of indole-3-acetic acid, synthesis of siderophores, solubilization of phosphates and soil pathogens inhibition. It can be suggested that strains of B. subtilis, B. amyloliquefaciens, B. velezensis, and B. licheniformis were positive for all the above traits, which have potential to be used as plant growth promoting inoculants to improve ginseng crop in the future.
Keywords
diversity; ginseng; plant growth promoting rhizobacteria (PGPR); rhizobacteria;
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1 Hynes, R.K., Leung, G.C., Hirkala, D.L., and Nelson, L.M. 2008. Isolation, selection, and characterization of beneficial rhizobacteria from pea, lentil, and chickpea grown in western Canada. Can. J. Microbiol. 54, 248-258.   DOI
2 Joshi, P. and Bhatt, A.B. 2011. Diversity and function of plant growth promoting rhizobacteria associated with wheat rhizosphere in North Himalayan region. Int. J. Environ. Sci. 1, 1135-1143.
3 Joshi, P., Tyagi, V., and Bhatt, A.B. 2011. Characterization of rhizobacteria diversity isolated from Oryza sativa cultivated at different altitude in North Himalaya. Adv. Appl. Sci. Res. 4, 208-216.
4 Lee, C.S., Kim, K.D., Hyun, J.W., and Jeun, Y.C. 2003. Isolation of rhizobacteria in Jeju island showing anti-fungal effect against fungal plant pathogens. Mycobiol. 31, 251-254.   DOI
5 Lee, S., Ka, J.-O., and Song, H.-G. 2012. Growth promotion of Xanthium italicum by application of rhizobacterial isolates of Bacillus aryabhattai in microcosm soil. J. Microbiol. 50, 45-49.   DOI
6 Lugtenberg, B. and Kamilova, F. 2009. Plant-growth-promoting rhizobacteria. Annu. Rev. Microbiol. 63, 541-556.   DOI
7 McGinnis, S. and Madden, T.L. 2004. BLAST: at the core of a powerful and diverse set of sequence analysis tools. Nucleic Acids Res. 32, 20-25.
8 Nautiyal, C.S. 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol. Lett. 170, 265-270.   DOI   ScienceOn
9 Noura, R., Ameur, C., Abdellatif, B., and Daniele, D. 2008. Screening of plant growth promoting traits of Bacillus thuringiensis. Ann. Microbiol. 58, 47-52.   DOI
10 Pereira, P., Ibanez, F., Rosenblueth, M., Etcheverry, M., and Martinez- Romero, E. 2011. Analysis of the bacterial diversity associated with the roots of Maize (Zea mays L.) through culture-dependent and culture-independent methods. ISRN Ecology, Volume 2011, Article ID 938546, 10 pages, doi:10.5402/2011/938546.   DOI
11 Ramos, B., Pozuelo, J.M., Acero, N., and Gutierrez Manero, F.J. 1998. Seasonal variation of Bacillus isolates from the rhizosphere of Elaeagnus angustifolia L. Orsis. 13, 7-16.
12 Rroco, E., Kosegarten, H., Harizaj, F., Imani, J., and Mengel, K. 2003. The importance of soil microbial activity for the supply of iron to sorghum and rape. Europ. J. Agron. 19, 487-493.   DOI
13 Sadfi, N., Cherif, M., Hajlaoui, M.R., Boudabbous, A., and Belanger, R. 2002. Isolation and partial purification of antifungal metabolites produced by Bacillus cereus. Ann. Microbiol. 52, 323-337.
14 Schwyn, B. and Neilands, J.B. 1987. Universal chemical assay for the detection and determination of siderophores. Anal. Biochem. 160, 47 -56.   DOI   ScienceOn
15 Selvadurai, E.L., Brown, A.E., and Hamilton, J.T.G. 1991. Production of indole-3-acetic acid analogues by strains of Bacillus cereus in relation to their influence on seedling development. Soil Biol. Biochem. 23, 401-403.   DOI
16 Sharma, A., Johri, B.N., Sharma, A.K., and Glick, B.R. 2003. Plant growth promoting bacterium Pseudomonas sp., strain GRP3 influences iron acquisition in mung bean (Vigna radiate L. Wilzeck). Soil Biol. Biochem. 35, 887-894.   DOI
17 Tamura, K., Dudley, J., Nei, M., and Kumar, S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596-1599.   DOI   ScienceOn
18 Tompson, J.D., Higgins, D.G., and Gibson, T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680.   DOI   ScienceOn
19 Bai, Y., Frederic, D.A., Donald, L.S., and Brian, T.D. 2002. Isolation of plant-growth-promoting Bacillus strains from soybean root nodules. Can. J. Microbiol. 48, 230-238.   DOI
20 Bashan, Y., Holguin, G., and de-Bashan, L.E. 2004. Azospirillum-plant relationships: physiological, molecular, agricultural, and environmental advances. Can. J. Microbiol. 50, 521-577.   DOI
21 Vendan, R.T., Yu, Y.J., Lee, S.H., and Rhee, Y.H. 2010. Diversity of endophytic bacteria in ginseng and their potential for plant growth promotion. J. Microbiol. 48, 559-565.   DOI
22 Vessey, J.K. 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255, 571-586.   DOI   ScienceOn
23 Yang, J., Kloepper, J.W., and Ryu, C.M. 2009. Rhizosphere bacteria help plants tolerate abiotic stress. Trends Plant Sci. 14, 1-4.   DOI
24 Yu, W.J., Lee, B.J., Nam, S.Y., Yang, D.C., and Yun, Y.W. 2003. Modulating effects of Korean ginseng saponins on ovarian function immature rats. Biol. Pharm. Bull. 26, 2574-2580.
25 Chang, W.T., Chen, Y.C., and Jao, C.L. 2007. Antifungal activity and enhancement of plant growth by Bacillus cereus grown on shellfish chitin wastes. Bioresour. Technol. 98, 1224-1230.   DOI
26 Banchio, E., Bogino, P.C., Zygadlo, J., and Giordano, W. 2008. Plant growth promoting rhizobacteria improve growth and essential oil yield in Origanum majorana L. Biochem. Syst. Ecol. 36, 766-771.   DOI
27 Calvo, P., Orrillo, E.O., Romero, E.M., and Zuniga, D. 2010. Characterization of Bacillus isolates of potato rhizosphere from Andean soils of Peru and their potential PGPR characteristics. Braz. J. Microbiol. 41, 899-906.   DOI
28 Chaiharn, M. and Lumyong, S. 2009. Phosphate solubilization potential and stress tolerance of rhizobacteria from rice soil in Northern Thailand. World J. Microbiol. Biotechnol. 25, 305-314.   DOI
29 Chatli, A.S., Beri, V., and Sidhu, B.S. 2008. Isolation and characterisation of phosphate solubilising microorganisms from the cold desert habitat of Salix alba Linn. in trans Himalayan region of Himachal Pradesh. Indian J. Microbiol. 48, 267-273.   DOI   ScienceOn
30 Goldstein, A.H. 1986. Bacterial solubilization of mineral phosphates: historical perspective and future prospects. Am. J. Alter. Agric. 1, 51-57.   DOI
31 Han, J., Xia, D., Li, L., Sun, L., Yang, K., and Zhang, L. 2009. Diversity of culturable bacteria isolated from root domains of Moso Bamboo (Phyllostachys edulis). Microb. Ecol. 58, 363-373.   DOI
32 Hartmann, A., Singh, M., and Klingmueller, W. 1983. Isolation and characterization of Azospirillum mutants excreting high amounts of indole acetic acid. Can. J. Microbiol. 29, 916-923.   DOI