Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Occurrence of Stone Fruit Viruses on Peach Trees (Prunus persica L. Batsch) in Korea

국내에서 발생하는 복숭아 바이러스병

  • Cho, In Sook (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Cho, Jeom Doeg (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Choi, Seung Kook (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Choi, Gug Seoun (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 조인숙 (국립원예특작과학원 원예특작환경과) ;
  • 조점덕 (국립원예특작과학원 원예특작환경과) ;
  • 최승국 (국립원예특작과학원 원예특작환경과) ;
  • 최국선 (국립원예특작과학원 원예특작환경과)
  • Received : 2012.10.11
  • Accepted : 2012.11.14
  • Published : 2012.12.31
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Abstract

To investigate the occurrence of viruses in peach, leaf samples were collected from peach trees in commercial orchard of six areas in Korea. Reverse transcription polymerase chain reaction (RT-PCR) was used to identify the presence of the following stone fruit viruses: Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV) and Plum pox virus (PPV). About 65.0% of the 515 samples were infected with ACLSV and PNRSV. Virus-like symptoms showing mosaic on leaves was observed in ACLSV infected peach trees. However, PNRSV infected peach trees showed no symptoms. These viral DNAs by sequence analysis were confirmed 4 ACLSV isolates and 3 PNRSV isolates. The Korean peach isolates of ACLSV and PNRSV showed 70-99% and 88-99% amino acid sequence identities, respectively, with those reported previously and their amino acid sequence identities with each other were approximately 95% and 88%, respectively. Phylogenetic analysis indicated that the Korean ACLSV isolates belong to the A group of ACLSV. The Korean PNRSV isolates reported in this study were grouped into I (PV32), II (PV96) and III (PE5) groups.

국내에서 재배되는 복숭아에 발생하는 바이러스병을 조사하기 위하여 경북 영천 등 복숭아 주산단지 6개 지역에서 전체 시료 515점을 채집하여 5종(ACLSV, ApMV, PDV, PNRSV, PPV) 바이러스의 감염여부를 RT-PCR 방법을 이용하여 바이러스 검정을 실시하였다. RT-PCR 검정 결과 전체 시료 515점 중 335점의 시료에서 ACLSV와 PNRSV가 검출되어 검정한 시료의 65.0%가 바이러스에 감염된 것을 확인하였다. ACLSV가 검출된 복숭아나무는 잎에 모자이크 증상이 관찰되었으며 PNRSV가 검출된 복숭아나무는 별다른 이상증상이 관찰되지 않았다. 검출된 바이러스의 유전자 염기서열 분석으로 ACLSV 4개와 PNRSV 3개의 분리주들을 확인하였으며 ACLSV 분리주들 간에는 95% 이상, PNRSV는 88% 이상의 아미노산 서열 상동성을 나타냈다. 기존에 보고된 ACLSV 및 PNRSV 분리주들과의 아미노산 서열 비교에서는 ACLSV 분리주들의 경우 70-99%, PNRSV 분리주들의 경우 88-99%의 상동성을 보였다. 이들 바이러스 분리주들의 계통학적 분석에서 ACLSV 분리주들은 A, B 그룹 중 A그룹에, PNRSV 분리주들은 I (PV32), II (PV96), III (PE5) 그룹에 각각 하나씩 속하는 것으로 나타났다.

Keywords

References

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