Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Development of a Qualitative Dose Indicator for Gamma Radiation Using Lyophilized Deinococcus

  • Lim, Sangyong (Research Division for Biotechnology, Korea Atomic Energy Research Institute) ;
  • Song, Dusup (Research Division for Biotechnology, Korea Atomic Energy Research Institute) ;
  • Joe, Minho (Research Division for Biotechnology, Korea Atomic Energy Research Institute) ;
  • Kim, Dongho (Research Division for Biotechnology, Korea Atomic Energy Research Institute)
  • Received : 2012.02.22
  • Accepted : 2012.05.04
  • Published : 2012.09.28
Download PDF
⟨ Previous Next ⟩

Abstract

The feasibility of using Deinococcus showing strong resistance to both desiccation and ionizing radiation as a dose indicator of gamma radiation exposure was evaluated. Three Deinococcus strains having different levels of radiation resistance, Deinococcus radiodurans (DRD), Deinococcus radiopugnans (DRP), and the DRD pprI mutant (DRM), were selected to develop an appropriate dose indicator for a broad range of exposures. DRD, DRP, and DRM cultures with different numbers of cells [${\sim}10^7$ to $10^3$ colony forming units (CFU)/$100{\mu}l$] were lyophilized and subjected to various doses of gamma radiation to determine a critical dose that inhibited bacterial growth completely. Finally, a combination of DRD at ${\sim}10^7$ and ${\sim}10^6$ CFU, DRP at ${\sim}10^5$ CFU, and DRM at ${\sim}10^4$ CFU successfully indicated exposure to 5, 10, 20, and 30 kGy of gamma radiation, respectively. This study shows the possibility of developing a qualitative indicator of radiation exposure using Deinococcus.

Keywords

References

  1. Bauermeister, A., R. Moeller, G. Reitz, S. Sommer, and P. Rettberg. 2011. Effect of relative humidity on Deinococcus radiodurans' resistance to prolonged dessication, heat, ionizing, germicidal, and environmentally relevant UV radiation. Microb. Ecol. 61: 715-722. https://doi.org/10.1007/s00248-010-9785-4
  2. Chen, Z.-M., Q. Li, H.-M. Liu, N. Yu, T.-J. Xie, M.-Y. Yang, et al. 2010. Greater enhancement of Bacillus subtilis spore yields in submerged cultures by optimization of medium composition through statistical experimental designs. Appl. Microbiol. Biotechnol. 85: 1353-1360. https://doi.org/10.1007/s00253-009-2162-x
  3. Cox, M. M. and J. R. Battista. 2005. Deinococcus radiodurans - The consummate survivor. Nat. Rev. Microbiol. 3: 882-892. https://doi.org/10.1038/nrmicro1264
  4. Funayama, T., I. Narumi, M. Kikuchi, S. Kitayama, H. Watanabe, and K. Yamamoto. 1999. Identification and disruption analysis of the recN gene in the extremely radioresistant bacterium Deinococcus radiodurans. Mutat. Res. 435: 151-161. https://doi.org/10.1016/S0921-8777(99)00044-0
  5. Hammad, A. A. 2008. Microbiological aspects of radiation sterilization, pp. 119-128. In: Trends in Radiation Sterilization of Health Care Products. International Atomic Energy Agency, Vienna, Austria.
  6. Hua, Y., I. Narumi, G. Gao, B. Tian, K. Satoh, S. Kitayama, and B. Shen. 2003. PrrI: A general switch responsible for extreme radioresistance of Deinococcus radiodurans. Biochem. Biophys. Res. Commun. 306: 354-360. https://doi.org/10.1016/S0006-291X(03)00965-3
  7. Jung, S., M. Joe, S. Im, S. D. Kim, and S. Lim. 2010. Comparison of the genomes of Deinococcal species using oligonucleotide microarrays. J. Microbiol. Biotechnol. 20: 1637-1646.
  8. Mattimore, V. and J. R. Battista. 1996. Radioresistance of Deinococcus radiodurans: Functions necessary to survive ionizing radiation are also necessary to survive prolonged desiccation. J. Bacteriol. 178: 633-637. https://doi.org/10.1128/jb.178.3.633-637.1996
  9. Parish, A. N. 1981. Effects of Gamma Radiation on Bacillus pumilus E601 A Biological Indicator for Irradiation Sterilization. University Microfilms International, Ann Arbor, Michigan.
  10. Satoh, K., Z. Tu, H. Ohba, and I. Narumi. 2009. Development of versatile shuttle vectors for Deinococcus grandis. Plasmid 62: 1-9. https://doi.org/10.1016/j.plasmid.2009.01.005
  11. Sella, S. R. B. R., B. P. Guizelini, L. P. S. Vandenberghe, A. B. P. Medeiros, and C. R. Soccol. 2009. Bioindicator production with Bacillus atrophaeus' thermal-resistant spores cultivated by solid-state fermentation. Appl. Microbiol. Biotechnol. 82: 1019-1026. https://doi.org/10.1007/s00253-008-1768-8
  12. Silindir, M. and Y. Ozer. 2009. Sterilization methods and the comparison of E-beam sterilization with gamma radiation sterilization. FABAD J. Pharm. Sci. 34: 43-53.
  13. Biological indicators for sterilization, U.S. Pharmacopeia. Retrieved April 27, 2012, from http://www.pharmacopeia.cn/v29240/usp29nf24s0_c1035.html.

Cited by

  1. Deinococcus swuensis sp. nov., a gamma-radiation-resistant bacterium isolated from soil vol.51, pp.3, 2013, https://doi.org/10.1007/s12275-013-3023-y
  2. Paenibacillus swuensis sp. nov., a Bacterium Isolated from Soil vol.52, pp.2, 2012, https://doi.org/10.1007/s12275-014-3546-x
  3. Deinococcus puniceus sp. nov., a Bacterium Isolated from Soil-Irradiated Gamma Radiation vol.70, pp.4, 2012, https://doi.org/10.1007/s00284-014-0748-8
  4. Expression and Mutational Analysis of DinB-Like Protein DR0053 in Deinococcus radiodurans vol.10, pp.2, 2012, https://doi.org/10.1371/journal.pone.0118275