Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enzymatic Synthesis of Puerarin Glucosides Using Leuconostoc Dextransucrase

  • Ko, Jin-A (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ryu, Young Bae (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Park, Tae-Soon (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Jeong, Hyung Jae (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Jang-Hoon (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Park, Su-Jin (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Joong-Su (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Doman (School of Biological Sciences and Technology and the Research Institute for Catalysis, Chonnam National University) ;
  • Kim, Young-Min (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Woo Song (Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • Received : 2012.02.06
  • Accepted : 2012.04.22
  • Published : 2012.09.28
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Abstract

Puerarin (P), an isoflavone derived from kudzu roots, has strong biological activities, but its bioavailability is often limited by its low water solubility. To increase its solubility, P was glucosylated by three dextransucrases from Leuconostoc or Streptococcus species. Leuconostoc lactis EG001 dextransucrase exhibited the highest productivity of puerarin glucosides (P-Gs) among the three tested enzymes, and it primarily produced two P-Gs with a 53% yield. Their structures were identified as ${\alpha}$-$_D$-glucosyl-($1{\rightarrow}6$)-P (P-G) by using LC-MS or $^1H$- or $^{13}C$-NMR spectroscopies and ${\alpha}$-$_D$-isomaltosyl-($1{\rightarrow}6$)-P (P-IG2) by using specific enzymatic hydrolysis, and their solubilities were 15- and 202-fold higher than that of P, respectively. P-G and P-IG2 are easily applicable in the food and pharmaceutical industries as alternative functional materials.

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References

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