Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Determination of epidemiological tetracycline MIC cut-off value for Vibrio ichthyoenteri

  • Han, Hyun-Ja (National Fisheries Research and Development Institute) ;
  • Kim, Do-Hyung (Department of Aqualife Medicine, Chonnam National University) ;
  • Lee, Deok-Chan (National Fisheries Research and Development Institute) ;
  • Won, Kyoung-Mi (National Fisheries Research and Development Institute) ;
  • Lee, Soon-Jeong (National Fisheries Research and Development Institute) ;
  • Cho, Mi-Young (National Fisheries Research and Development Institute) ;
  • Jee, Bo-Young (National Fisheries Research and Development Institute) ;
  • Kim, Jin-Woo (National Fisheries Research and Development Institute)
  • Received : 2011.06.30
  • Accepted : 2011.08.04
  • Published : 2011.08.31
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Abstract

Normalized resistance interpretation (NRI) analysis for tetracycline was applied to generate information on the epidemiological cut-off value for Vibrio ichthyoenteri isolated from diseased olive flounder (Paralichthys olivaceus) larvae. Thus, 42 strains of V. ichthyoenteri were used to determine minimum inhibitory concentration (MIC) values of tetracycline using Etest. Also, 11 tetracycline resistance related genes were investigated by PCR method. Most tetracycline-resistant strains harbored both tetB and tetM with a few exceptions. NRI-derived mean and 2 SD above the mean of theoretical normal distributions of susceptible isolates were 0.33 mg/L and 1.66 mg/L, respectively. The epidemiological cut-off value for V. ichthyoenteri from the calculations could be set to S ${\leq}$ 2 mg/L. Of the 42 strains, 15 were classified as non-wild type (NWT), and MIC values of the NWT strains vary regardless of tetB and tetM detection, suggesting that there may be other mechanisms involved in tetracycline resistance in this Vibrio species.

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References

  1. Agerso, Y. and Petersen, A.: The tetracycline resistance determinant Tet39 and the sulphonamide resistance gene sulII are common among resistant Acinetobacter spp. isolated from integrated fish farms in Thailand. J. Antimicrob Chemother., 59: 23-27, 2007.
  2. Aminov, R.I., Chee-Sandford, J.C., Garrigues, N., Teferedegne, B., Krapac, I.J., White, B.A. and Macki, R.I.: Development, validation and application of PCR primers for detection of tetracycline efflux genes of gram negative bacteria. Appl. Environ. Microbiol., 68: 1786-1793, 2002. https://doi.org/10.1128/AEM.68.4.1786-1793.2002
  3. Avendano-Herrera, R., Molina, A., Magarinns, B., Toranzo, A.E. and Smith, P.: Estimation of epidemiological cut-off values for disk diffusion susceptibility test data for Streptococcus phocae. Aquaculture, 314: 44-48, 2011. https://doi.org/10.1016/j.aquaculture.2011.01.049
  4. CLSI: Clinical and Laboratory Standards Institute, Wayne, Pennsylvania: Methods for antimicrobial disk susceptibility testing of bacteria isolated from aquatic animals. Approved guideline M42-A, 2006.
  5. CLSI: Clinical and Laboratory Standards Institute, Wayne, Pennsylvania: Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated of fastidious bacteria; Approved guideline M45-A2, 2010.
  6. Jun, L.J, Jeong, J.B., Huh, M.-D., Chung, J.-K., Choi, D.-L., Lee, C.-H. and Jeong, H.D.: Detection of tetracycline-resistance determinants by multiplex polymerase chain reaction in Edwardsiella tarda isolated from fish farms in Korea. Aquaculture, 240: 89-100, 2004. https://doi.org/10.1016/j.aquaculture.2004.07.025
  7. Kim, D.H., Han, H.J., Kim, S.M., Lee, D.C. and Park, S.I.: Bacterial enteritis and the development of the larval digestive tract in olive flounder, Paralichthys olivaceus (Temminck & Schlegel). J. Fish Dis., 27: 497-505, 2004. https://doi.org/10.1111/j.1365-2761.2004.00553.x
  8. Kim, S.R., Nonake, L. and Suzuki, S.: Occurrence of tetracycline resistance genes tet(M) and tet(S) in bacteria from marine aquaculture sites. FEMS Microbiol., 237: 147-156, 2004. https://doi.org/10.1111/j.1574-6968.2004.tb09690.x
  9. Kim, Y.H., Jun, L.J., Park, S.H., Yoon, S. H., Chung, J.K., Kim, J.C. and Jeong, H.D.: Prevalence of tet(B) and tet(M) genes among tetracycline-resistant Vibrio spp. in the aquatic environments of Korea. Dis. Aquat. Org., 75: 209-216, 2007. https://doi.org/10.3354/dao075209
  10. Kronvall, G.: Determination of the real standard distribution of susceptible strains in zone histograms. Int. J. Antimicrob. Agents, 22: 7-13, 2003. https://doi.org/10.1016/S0924-8579(03)00107-9
  11. Kronvall, G., Kahlmeter, G., Myhre, E. and Galas, M.F.: A new method for normalized interpretation of antimicrobial resistance from disk test results for comparative purposes. Clin. Microbiol. Infect. 9: 120-132, 2003. https://doi.org/10.1046/j.1469-0691.2003.00546.x
  12. Kronvall, G., Karlsson, I, Walder, M., Sorberg, M. and Nilsson, L.: Epidemiological MIC cut-off values for tigecycline calculated from Etest MIC values using normalized resistance interpretation. J. Antimicrob Chemother., 57: 498-505, 2006. https://doi.org/10.1093/jac/dki489
  13. Kronvall, G.: Normalized resistance interpretation as a tool for establishing epidemiological MIC susceptibility breakpoints. J.Clin. Microbiol., 48: 4445-4452, 2010. https://doi.org/10.1128/JCM.01101-10
  14. Macovei, L. and Zurek, L.: Ecology of antibiotic resistance genes: characterization of enterococci from houseflies collected in food settings. Appl. Environ. Microbiol., 72: 4688-4694, 2006. https://doi.org/10.1128/AEM.02975-05
  15. Muroga, K., Yasunobu, H., Okada, N. and Masumura, K.: Bacterial enteritis of cultured flounder Paralichthys olivaceus larvae. Dis. Aquat. Org., 9: 121-125, 1990. https://doi.org/10.3354/dao009121
  16. Ng, L.K., Martin, I., Alfa, M. and Mulvey, M.: Multiplex PCR for the detection of tetracycline resistance genes. Mol. Cell. Probes, 15: 209-215, 2001. https://doi.org/10.1006/mcpr.2001.0363
  17. Roberts, M.C.: Update on acquired tetracycline resistance gene. FEMS Microbiol. Lett. 245: 195-203, 2005. https://doi.org/10.1016/j.femsle.2005.02.034
  18. Smith, P., Douglas, I, McMurray, J. and Carroll, C.: A rapid method of improving the criteria being used to interpret disc diffusion antimicrobial susceptibility test data for bacteria associated with fish diseases, Aquaculture, 290: 172-178, 2009. https://doi.org/10.1016/j.aquaculture.2009.02.017
  19. Smith, P. and Christofilogiannis, P.: Application of normalised resistance interpretation to the detection of multiple low-level resistance in strains of Vibrio anguillarum obtained from Greek fish farms. Aquaculture, 272: 223-230, 2007. https://doi.org/10.1016/j.aquaculture.2007.07.232
  20. Smith, P., Ruane, N.M., Douglas, I., Carroll, C., Kronvall, G. and Gerard, T.A.: Fleming impact of inter-lab variation on the estimation of epidemiological cut-off values for disc diffusion susceptibility test data for Aeromonas salmonicida. Aquaculture, 272: 168-179, 2007. https://doi.org/10.1016/j.aquaculture.2007.07.216
  21. Whittle, G., Whitehead, T.R., Hamburger, N., Shoemarker, N.B., Cotta, M.A. and Salyers, A.A.: Identification of a new ribosomal protection type of tetracycline resistance gene, tet(36), from swine manure pits. Appl. Environ. Microbiol., 69: 4151-4159, 2003. https://doi.org/10.1128/AEM.69.7.4151-4158.2003