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http://dx.doi.org/10.7847/jfp.2011.24.2.095

Determination of epidemiological tetracycline MIC cut-off value for Vibrio ichthyoenteri  

Han, Hyun-Ja (National Fisheries Research and Development Institute)
Kim, Do-Hyung (Department of Aqualife Medicine, Chonnam National University)
Lee, Deok-Chan (National Fisheries Research and Development Institute)
Won, Kyoung-Mi (National Fisheries Research and Development Institute)
Lee, Soon-Jeong (National Fisheries Research and Development Institute)
Cho, Mi-Young (National Fisheries Research and Development Institute)
Jee, Bo-Young (National Fisheries Research and Development Institute)
Kim, Jin-Woo (National Fisheries Research and Development Institute)
Publication Information
Journal of fish pathology / v.24, no.2, 2011 , pp. 95-102 More about this Journal
Abstract
Normalized resistance interpretation (NRI) analysis for tetracycline was applied to generate information on the epidemiological cut-off value for Vibrio ichthyoenteri isolated from diseased olive flounder (Paralichthys olivaceus) larvae. Thus, 42 strains of V. ichthyoenteri were used to determine minimum inhibitory concentration (MIC) values of tetracycline using Etest. Also, 11 tetracycline resistance related genes were investigated by PCR method. Most tetracycline-resistant strains harbored both tetB and tetM with a few exceptions. NRI-derived mean and 2 SD above the mean of theoretical normal distributions of susceptible isolates were 0.33 mg/L and 1.66 mg/L, respectively. The epidemiological cut-off value for V. ichthyoenteri from the calculations could be set to S ${\leq}$ 2 mg/L. Of the 42 strains, 15 were classified as non-wild type (NWT), and MIC values of the NWT strains vary regardless of tetB and tetM detection, suggesting that there may be other mechanisms involved in tetracycline resistance in this Vibrio species.
Keywords
Vibrio ichthyoenteri; Olive flounder; Tetracycline; Normalized resistance interpretation; Epidemiological cut-off value;
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1 Smith, P., Ruane, N.M., Douglas, I., Carroll, C., Kronvall, G. and Gerard, T.A.: Fleming impact of inter-lab variation on the estimation of epidemiological cut-off values for disc diffusion susceptibility test data for Aeromonas salmonicida. Aquaculture, 272: 168-179, 2007.   DOI   ScienceOn
2 Whittle, G., Whitehead, T.R., Hamburger, N., Shoemarker, N.B., Cotta, M.A. and Salyers, A.A.: Identification of a new ribosomal protection type of tetracycline resistance gene, tet(36), from swine manure pits. Appl. Environ. Microbiol., 69: 4151-4159, 2003.   DOI   ScienceOn
3 Kronvall, G.: Normalized resistance interpretation as a tool for establishing epidemiological MIC susceptibility breakpoints. J.Clin. Microbiol., 48: 4445-4452, 2010.   DOI   ScienceOn
4 Macovei, L. and Zurek, L.: Ecology of antibiotic resistance genes: characterization of enterococci from houseflies collected in food settings. Appl. Environ. Microbiol., 72: 4688-4694, 2006.   DOI   ScienceOn
5 Muroga, K., Yasunobu, H., Okada, N. and Masumura, K.: Bacterial enteritis of cultured flounder Paralichthys olivaceus larvae. Dis. Aquat. Org., 9: 121-125, 1990.   DOI
6 Ng, L.K., Martin, I., Alfa, M. and Mulvey, M.: Multiplex PCR for the detection of tetracycline resistance genes. Mol. Cell. Probes, 15: 209-215, 2001.   DOI   ScienceOn
7 Roberts, M.C.: Update on acquired tetracycline resistance gene. FEMS Microbiol. Lett. 245: 195-203, 2005.   DOI   ScienceOn
8 Smith, P., Douglas, I, McMurray, J. and Carroll, C.: A rapid method of improving the criteria being used to interpret disc diffusion antimicrobial susceptibility test data for bacteria associated with fish diseases, Aquaculture, 290: 172-178, 2009.   DOI   ScienceOn
9 Jun, L.J, Jeong, J.B., Huh, M.-D., Chung, J.-K., Choi, D.-L., Lee, C.-H. and Jeong, H.D.: Detection of tetracycline-resistance determinants by multiplex polymerase chain reaction in Edwardsiella tarda isolated from fish farms in Korea. Aquaculture, 240: 89-100, 2004.   DOI   ScienceOn
10 Smith, P. and Christofilogiannis, P.: Application of normalised resistance interpretation to the detection of multiple low-level resistance in strains of Vibrio anguillarum obtained from Greek fish farms. Aquaculture, 272: 223-230, 2007.   DOI   ScienceOn
11 Kim, D.H., Han, H.J., Kim, S.M., Lee, D.C. and Park, S.I.: Bacterial enteritis and the development of the larval digestive tract in olive flounder, Paralichthys olivaceus (Temminck & Schlegel). J. Fish Dis., 27: 497-505, 2004.   DOI   ScienceOn
12 Kim, S.R., Nonake, L. and Suzuki, S.: Occurrence of tetracycline resistance genes tet(M) and tet(S) in bacteria from marine aquaculture sites. FEMS Microbiol., 237: 147-156, 2004.   DOI   ScienceOn
13 Kim, Y.H., Jun, L.J., Park, S.H., Yoon, S. H., Chung, J.K., Kim, J.C. and Jeong, H.D.: Prevalence of tet(B) and tet(M) genes among tetracycline-resistant Vibrio spp. in the aquatic environments of Korea. Dis. Aquat. Org., 75: 209-216, 2007.   DOI
14 Aminov, R.I., Chee-Sandford, J.C., Garrigues, N., Teferedegne, B., Krapac, I.J., White, B.A. and Macki, R.I.: Development, validation and application of PCR primers for detection of tetracycline efflux genes of gram negative bacteria. Appl. Environ. Microbiol., 68: 1786-1793, 2002.   DOI   ScienceOn
15 Kronvall, G.: Determination of the real standard distribution of susceptible strains in zone histograms. Int. J. Antimicrob. Agents, 22: 7-13, 2003.   DOI   ScienceOn
16 Kronvall, G., Kahlmeter, G., Myhre, E. and Galas, M.F.: A new method for normalized interpretation of antimicrobial resistance from disk test results for comparative purposes. Clin. Microbiol. Infect. 9: 120-132, 2003.   DOI   ScienceOn
17 Kronvall, G., Karlsson, I, Walder, M., Sorberg, M. and Nilsson, L.: Epidemiological MIC cut-off values for tigecycline calculated from Etest MIC values using normalized resistance interpretation. J. Antimicrob Chemother., 57: 498-505, 2006.   DOI   ScienceOn
18 Avendano-Herrera, R., Molina, A., Magarinns, B., Toranzo, A.E. and Smith, P.: Estimation of epidemiological cut-off values for disk diffusion susceptibility test data for Streptococcus phocae. Aquaculture, 314: 44-48, 2011.   DOI   ScienceOn
19 CLSI: Clinical and Laboratory Standards Institute, Wayne, Pennsylvania: Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated of fastidious bacteria; Approved guideline M45-A2, 2010.
20 CLSI: Clinical and Laboratory Standards Institute, Wayne, Pennsylvania: Methods for antimicrobial disk susceptibility testing of bacteria isolated from aquatic animals. Approved guideline M42-A, 2006.
21 Agerso, Y. and Petersen, A.: The tetracycline resistance determinant Tet39 and the sulphonamide resistance gene sulII are common among resistant Acinetobacter spp. isolated from integrated fish farms in Thailand. J. Antimicrob Chemother., 59: 23-27, 2007.