한국어병학회지 (Journal of fish pathology)

  • 제24권2호
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  • Pages.75-84
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  • 2011
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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양식 넙치, Paralichthys olivaceus에서 분리한 viral hemorrhagic septicemia virus (VHSV)의 항원성 분석

Analysis of antigenicity of viral hemorrhagic septicemia virus (VHSV) glycoprotein from cultured olive flounder Paralichthys olivaceus

  • 김수미 (국립수산과학원 서해수산연구소) ;
  • 지보영 (국립수산과학원 수산생물방역과) ;
  • 조미영 (국립수산과학원 수산생물방역과) ;
  • 원경미 (국립수산과학원 수산생물방역과) ;
  • 김진우 (국립수산과학원 수산생물방역과) ;
  • 박수일 (부경대학교 수산생명의학과)
  • Kim, Su-Mi (West Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Jee, Bo-Young (Aquatic Life Disease Control Division, National Fisheries Research and Development Institute) ;
  • Cho, Mi-Young (Aquatic Life Disease Control Division, National Fisheries Research and Development Institute) ;
  • Won, Kyoung-Mi (Aquatic Life Disease Control Division, National Fisheries Research and Development Institute) ;
  • Kim, Jin-Woo (Aquatic Life Disease Control Division, National Fisheries Research and Development Institute) ;
  • Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University)
  • 투고 : 2011.07.21
  • 심사 : 2011.08.16
  • 발행 : 2011.08.31
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초록

우리나라 양식 넙치에서 분리한 VHSV (KR'01-1)의 glycoprotein 아미노산 배열을 기초로 단백질 전환 구조상 유연성, 친수성 및 항원성이 높고, 표면에 노출되어 있을 가능성(surface probability)이 높다고 분석되는 3개의 peptide (Gp1, Gp2, Gp3)를 선정하였다. 정제한 바이러스 입자 및 3개의 합성 peptide에 대한 polyclonal 항체를 제작하여 항원성 분석을 실시한 결과, 합성 peptide로 제작한 모든 항혈청은 Western blotting 결과 VHSV의 구조단백질과 특이적으로 결합하는 나타났으며, 이 가운데 Gp1 및 Gp2 합성 peptide에 대한 항혈청은 양식 넙치에서 분리한 VHSV를 중화시키는 것으로 나타나, peptide-based antigen의 이용 가능성을 제시하였다.

The amino acid sequence of glycoprotein of Korean VHSV isolate (KR'01-1) was analyzed using the DNAStar Protean system. Based on the flexibility, hydrophilicity, antigenic index and surface probability, three regions (Gp1, Gp2 and Gp3) were selected as potential antigenic determinants. Three oligopeptides containing the amino acid sequences of the three regions were synthesized and polyclonal antibodies were raised against them. The activities of the antibodies were analyzed by Western blotting and virus neutralization test. The results showed that antibodies raised against oligopeptides Gp1 and Gp2 neutralized the infectivity of VHSV, suggesting that they can be possible candidates for subunit vaccines against VHS diseases in olive flounder.

키워드

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