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http://dx.doi.org/10.7847/jfp.2011.24.2.075

Analysis of antigenicity of viral hemorrhagic septicemia virus (VHSV) glycoprotein from cultured olive flounder Paralichthys olivaceus  

Kim, Su-Mi (West Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
Jee, Bo-Young (Aquatic Life Disease Control Division, National Fisheries Research and Development Institute)
Cho, Mi-Young (Aquatic Life Disease Control Division, National Fisheries Research and Development Institute)
Won, Kyoung-Mi (Aquatic Life Disease Control Division, National Fisheries Research and Development Institute)
Kim, Jin-Woo (Aquatic Life Disease Control Division, National Fisheries Research and Development Institute)
Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Journal of fish pathology / v.24, no.2, 2011 , pp. 75-84 More about this Journal
Abstract
The amino acid sequence of glycoprotein of Korean VHSV isolate (KR'01-1) was analyzed using the DNAStar Protean system. Based on the flexibility, hydrophilicity, antigenic index and surface probability, three regions (Gp1, Gp2 and Gp3) were selected as potential antigenic determinants. Three oligopeptides containing the amino acid sequences of the three regions were synthesized and polyclonal antibodies were raised against them. The activities of the antibodies were analyzed by Western blotting and virus neutralization test. The results showed that antibodies raised against oligopeptides Gp1 and Gp2 neutralized the infectivity of VHSV, suggesting that they can be possible candidates for subunit vaccines against VHS diseases in olive flounder.
Keywords
Viral hemorrhagic septicemia virus; Glycoprotein; Oligopeptide antigen;
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