Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Macromolecular Docking Simulation to Identify Binding Site of FGB1 for Antifungal Compounds

  • Soundararajan, Prabhakaran (Department of Horticulture, Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University) ;
  • Sakkiah, Sugunadevi (Division of Applied Life Science (BK21 Program), Systems and Synthetic Agrobiotech (SSAC), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Researh Institute of Natural Science (RINS), Gyeongsang National University (GNU)) ;
  • Sivanesan, Iyyakkannu (Institute of Agricultural & Life Science, Gyeongsang National University) ;
  • Lee, Keun-Woo (Division of Applied Life Science (BK21 Program), Systems and Synthetic Agrobiotech (SSAC), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Researh Institute of Natural Science (RINS), Gyeongsang National University (GNU)) ;
  • Jeong, Byoung-Ryong (Department of Horticulture, Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University)
  • Received : 2011.05.19
  • Accepted : 2011.08.16
  • Published : 2011.10.20
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Abstract

Fusarium oxysporum, an important pathogen that mainly causes vascular or fusarium wilt disease which leads to economic loss. Disruption of gene encoding a heterotrimeric G-protein-${\beta}$-subunit (FGB1), led to decreased intracellular cAMP levels, reduced pathogenicity, colony morphology, and germination. The plant defense protein, Nicotiana alata defensin (NaD1) displays potent antifungal activity against a variety of agronomically important filamentous fungi. In this paper, we performed a molecular modeling and docking studies to find vital amino acids which can interact with various antifungal compounds using Discovery Studio v2.5 and GRAMMX, respectively. The docking results from FGB1-NaD1 and FGB1-antifungal complexes, revealed the vital amino acids such as His64, Trp65, Ser194, Leu195, Gln237, Phe238, Val324 and Asn326, and suggested that the anidulafungin is a the good antifungal compound.The predicted interaction can greatly assist in understanding structural insights for studying the pathogen and host-component interactions.

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