Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Plant Regeneration From Mature Seed of Domestic Italian Ryegrass Cultivar

국내개발 이탈리안 라이그라스 품종 성숙종자의 식물체 재분화

  • Kim, Kyung-Hee (Dept. of Animal Biosciences, IALS, PMBBRC, Division of Applied Life Sciences (BK21), Gyeongsang National University) ;
  • Kim, Yong-Goo (Dept. of Animal Biosciences, IALS, PMBBRC, Division of Applied Life Sciences (BK21), Gyeongsang National University) ;
  • Heo, Sung-Hyun (Dept. of Animal Biosciences, IALS, PMBBRC, Division of Applied Life Sciences (BK21), Gyeongsang National University) ;
  • Lee, Ki-Won (National Institute of Animal Science, RDA) ;
  • Lee, Byung-Hyun (Dept. of Animal Biosciences, IALS, PMBBRC, Division of Applied Life Sciences (BK21), Gyeongsang National University)
  • 김경희 (경상대학교 응용생명과학부 동물생명과학과) ;
  • 김용구 (경상대학교 응용생명과학부 동물생명과학과) ;
  • 허성현 (경상대학교 응용생명과학부 동물생명과학과) ;
  • 이기원 (농촌진흥청 국립축산과학원) ;
  • 이병현 (경상대학교 응용생명과학부 동물생명과학과)
  • Received : 2011.08.10
  • Accepted : 2011.09.16
  • Published : 2011.09.30
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Abstract

In order to improve forage characteristics of Italian ryegrass by genetic transformation, an efficient callus induction from mature seed and optimal plant regeneration system were established using a domestic cultivar 'Kospeed'. Addition of 5 mg/L of 2,4-D showed highest frequency of embryogenic callus induction from mature seeds. N6 medium showed higher frequency of both callus induction and plant regeneration as compared with MS and SH medium. The highest plant regeneration frequency 67% was obtained when embryogenic calli were transferred to N6 medium containing 1 mg/L 2,4-D and 5 mg/L BA. Supplementation of regeneration medium with sucrose at 30 g/L level maximized regeneration frequency as compared to the other concentrations. These data would be very helpful for molecular breeding of domestic Italian ryegrass cultivar through genetic transformation.

국내 개발 재배품종인 코스피드 이탈리안 라이그라스의 최적 배양조건을 확립하기 위하여 성숙종자로부터 캘러스 유도조건 및 식물체 재분화 조건을 조사하였다. 캘러스 유도시 첨가되는 auxin으로는 2,4-D가 NAA 보다 효율적이었으며, 5 mg/L 2,4-D가 첨가된 N6 배지에서 캘러스가 가장 높은 빈도로 유도되었다. 캘러스로부터 식물체 재분화는 1 mg/L 2,4-D와 5mg/L BA가 첨가된 N6 배지에서 배양했을 때 67% 이상의 재분화율을 나타내었다. 기본배지의 종류에 따른 배양효율의 차이는 캘러스 유도와 식물체 재분화에는 N6배지가 효과적이었으며, 재분화배지에 첨가되는 탄소원에는 30g/L의 sucrose가 가장 높은 재분화 효율을 나타내었다. 본 연구를 통하여 확립된 고효율 재분화 시스템은 분자육종을 통한 신품종 이탈리안라이그라스의 실용화 연구에 큰 도움이 될 것으로 추측된다.

Keywords

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