한국연초학회지 (Journal of the Korean Society of Tobacco Science)

한국연초학회 (The Korean Society of Tobacco Science)
권호 표지

잎담배 단백태 질소 분석법(Mohr법) 개선

Optimum Determination Method of Protein-Nitrogen Improve Mohr Method in Leaf Tobacco

  • 투고 : 2011.11.01
  • 심사 : 2011.11.21
  • 발행 : 2011.12.24
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study was carried out in order to improve the Mohr method for protein-nitrogen concentration determining. Existing Mohr's method takes seven hours to analyze for one sample. In order to make up for these things tried to utilize filter bag(F75, ANKOM) for sample treatment. As a result of this implementation, have saved sample loss amount and analyzing time for a quarter comparing with Mohr's method. In addition, it have gotten high efficiency through five samples hydrolyze per one trial by using ANKOM hydrolysis system with 0.5% acetic acid. Besides, had a good reproducibility for analysis results in relation to nitrogen concentration determining with Dumas methodology. Thus, new Mohr's method takes one day to analyze for 40 sample. It is more efficiency about 6 times compare with existing Mohr's method. And, this modified Mohr's method was verified that is substitutable for the existing Mohr's method in statistical analysis.

키워드

참고문헌

  1. Drochioiu, G., Mangalagiu, I. and Tataru, V.(2000) Specific spectrophotometric determination of hydrocyanic acid in the environment. Andalyst, 200, 125. 939-941.
  2. HCN : Removal - Propeties - Analyses. http://legacy.libr
  3. Leffingwell, J.C. and Leffingwell, D.(1988) Chemical and sensory aspects of tobacco flavor. Rec. Adv. Tob. Sci., 14, 162-218.
  4. R. POWELL GAINES (1986) Determining Protein Nitrogen in Tobacco. http://legacy.library.ucsf.edu/tid/hnf81b00/pdf
  5. Protein Nirogen : Analyses.
  6. Determination of "Protein" Nitrogen using dry-block herters and auto analyses. (Sgaf93a99(legacy)-Bat.pdf)
  7. Determination of "Protein" Nitrogen.(Sgaf46a99 (legacy)-Bat.pdf)
  8. Tso, T.C. (1990) Production, physiology and biochemistry of tobacco plant. institute of international development & education in agricultural and life sciences, USA.610.
  9. Tso, T. C. (1977) Tobacco as potential Food Source and Smoke Material. Beitr. Tabakforsch. 9, 63
  10. 한국연초학회 (2009) 담배과학, 123-124.
  11. 한국연초학회 (1987) 담배과학총론, 411.
  12. 한국인삼연초연구소 (1991) 담배성분분석법, 38-45.