농약과학회지 (The Korean Journal of Pesticide Science)

  • 제15권2호
  • /
  • Pages.194-200
  • /
  • 2011
  • /
  • 1226-6183(pISSN)
  • /
  • 2287-2051(eISSN)
한국농약과학회 (The Korean Society of Pesticide Science)
권호 표지

알루미늄호일 이용 배추좀나방(Plutella xylostella L.) 살란활성 검정법 개발 및 살란활성 식물추출물의 선발

Development of a Bioassay Method Using Aluminium Foil Sheet for Screening Ovicidal Activity Against Diamondback Moth Egg, Plutella xylostella L. and Selection of Plant Extracts with High Ovicidal Activity

  • 권민 (농촌진흥청 국립식량과학원 고령지농업연구센터) ;
  • 김주일 (농촌진흥청 국립식량과학원 고령지농업연구센터) ;
  • 이승환 (서울대학교 농업생명과학대학 농업생명공학부) ;
  • 임매영 (아주채소연구개발중심(AVRDC) 곤충과)
  • Kwon, Min (Highland Agriculture Research Center, National Institute of Highland Agriculture, RDA) ;
  • Kim, Ju-Il (Highland Agriculture Research Center, National Institute of Highland Agriculture, RDA) ;
  • Lee, Seung-Hwan (Lab. of Insect Biosystematics, School of Agricultural Biotech., Seoul National University) ;
  • Lin, Mei-Ying (Entomology Unit, Asian Vegetable Research & Development Center)
  • 투고 : 2011.05.26
  • 심사 : 2011.06.10
  • 발행 : 2011.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

배추좀나방(Plutella xylostella L.)은 세계적으로 배추과 작물의 가장 문제 해충이다. 지금까지 배추좀나방 방제는 유충을 대상으로 이루어졌으나, 더욱 효과적인 방제를 위해서 알의 부화를 억제하거나 살란활성을 가진 새로운 살충제 개발이 요구된다. 이에 알루미늄 호일을 활용하여 배추좀나방의 살란활성을 효율적으로 생물검정하는 방법을 개발하였다. 알루미늄호일($4{\times}12$ cm, 산란호일)에 배추잎 즙액을 도포하여 아크릴원통에 넣고 갓 우화한 성충 300마리를 24시간 접종한 결과 알을 충분하게 확보할 수 있었으며, 이는 4일간 연속으로 채란 가능하였다. 살란활성 검정은 난괴 크기별로 산란호일을 6~12조각으로 나눈 농도별로 준비된 메탄올 용액에 2~10초간 핀셋으로 침지한 후 음건(15~30분)하여 소형사육통에 넣어 부화율을 조사하였다. 산란호일은 50% 메탄올에 10초간 침지하여도 활성검정에 어떠한 영향도 미치지 않았다. 하지만 소형 사육통의 뚜껑을 개폐하면 활성물질의 성질에 따라 영향을 받았다 이러한 방법으로 식물체 50종의 메탄올 추출물에 대한 배추좀나방 살란활성을 검정한 결과, 고본(Angelica tenuissima) 뿌리, 원지(Polygala tenuifolia) 뿌리, 지골피(Lycium chinense) 뿌리, 천궁(Cnidium officinale) 뿌리 등은 90% 이상의 높은 살란활성을 나타내었다.

Diamondback moth (DBM, Plutella xylostella L.) is known as the most destructive pest of cruciferous crops worldwide. As most insecticides targeted to mainly larval stage, new insecticides which have hatching-inhibitory or ovicidal activity could be more efficient to control DBM. Therefore, we developed an easy and efficient method for screening ovicidal activity of DBM eggs using aluminum foil. The aluminum foil ($4{\times}12$ cm) coated with filtered juice of chinese cabbage leave (hereinafter called oviposition foil) exposed to 300 newly-emerged adults for 24 hours inside the rearing container. The oviposition foils were replaced every 4 days consecutively after mating, but it was better to discarded over then. Oviposition foil were divided into 6 to 12 pieces depending on egg mass volume. After dipping into test solutions for 10 seconds using faucet, oviposition foil pieces were placed into common petri dish, and then investigated hatchability. The effect of methanol solvent (50%) for 10 seconds dipping on the toxicity against DBM eggs was negligible. In addition, whether covering the petri dish or not should be dependent on nature of active compounds tested. With applying the new bioassay method, methanol extracts from 50 plants were tested the ovicidal activity to DBM eggs. Among them, four plant extracts; Angelica tenuissima root, Lycium chinense root, Cnidium officinale root and Polygala tenuifolia root, showed high ovicidal activity of over 90% control efficacy, against DBM eggs.

Keywords

References

  1. AVRDC (1997) Rearing of diamondback moth parasites. pp. 7-11. Asian Vegetable Research and Development Center, Taiwan.
  2. Dickson, M. H., A. M. Shelton, S. D. Eigenbrode, M. L. Vamosy and M. Mora (1990) Breeding for resistance to diamondback moth, Plutella xylostella, in cabbage. HortSci. 25:1643-1646.
  3. Gu, H. (2009) Cold tolerance and overwintering of the diamondback moth (Lepidoptera: Plutellidae) in Southeastern Australia. Environ. Entomol. 38(3):524-529. https://doi.org/10.1603/022.038.0303
  4. Hama, H. (1986) Resistance spectrum to various insecticides in the diamondback moth, Plutella xylostella L. Appl. Entomol. Zool. 30:277-284. https://doi.org/10.1303/jjaez.30.277
  5. Hines, R. L. and W. D. Hutchinson (2001) Evaluation of action thresholds and spinosad for lepidopteran pest management in Minnesota cabbage. J. Econ. Entomol 94(1):190-196. https://doi.org/10.1603/0022-0493-94.1.190
  6. Kim, M. H. (1990) Ecological study on diamondback moth (Plutella xylostella L.) at southern Korean Peninsula. M.S. Thesis. Chunnam Nat'l Univ., Korea.
  7. Krishnakumar, N. K., K. Srinivasan, C. L. Suman and P. R. Ramachander (1986) Optimum control strategy of cabbage pests from a chemical control trial. Prog. Hort. 18:104-110.
  8. Liu, M. Y. and C. N. Sun (1984) Rearing DBM (Lepidoptera: Yponomeutidae) on rape seedling by a modification of the Koshihara and Yamada method. J. Econ. Entomol. 77:1608-1609. https://doi.org/10.1093/jee/77.6.1608
  9. Matsumura, F. (1985) Classification of insecticides. pp. 45-109, In Toxicology of Insecticides (2nd edition). Plenum Press, New York.
  10. Nemoto, H., E. Yano and K. Kiritani (1992) Pheromonal control of diamondback moth in the management of crucifer pests. pp. 185-194, In Diamondback moth and other crucifer pests: Proceeding of the Second International Workshop (ed. N.S. Talekar). Asian Vegetable Research and Development Center, Taiwan.
  11. Schmutterer, H. (1992) Control of diamondback moth by application of neem extracts. pp.325-332, in Diamondback moth and other crucifer pests: Proceeding of the Second International Workshop (ed. N. S. Talekar). Asian Vegetable Research and Development Center, Taiwan.
  12. Shelton, A. M. and J. A. Wyman (1992) Insecticide resistance of diamondback moth in North America. pp. 447-454, In Management of Diamondback Moth and Other Crucifer Pests: Proceedings of Second International Workshop (ed. N. S. Talekar). Asian Vegetable Research and Development Center, Taiwan.
  13. Suenaga, H., A. Tanaka, M. Murata and M. Horikiri (1992) Development of a chitin synthesis inhibitor resistance in the DBM, Plutella xylostella (Lepidoptera: Plutellidae). Proc. Assoc. Pl. Prot. Kyushu. 38:129-131. https://doi.org/10.4241/kyubyochu.38.129
  14. Tabashnik, B. E., N. Finson, F. R. Greeters, W. J. Moar, M. W. Johnson, K. Luo and M. J. Adang (1994) Reversal of resistance to Bacillus thuringiensis in Plutella xylostella (Lepidoptera: Plutellidae). Proc. Natl. Acad. Sci. USA. 91:4120-4124. https://doi.org/10.1073/pnas.91.10.4120
  15. Talekar, N. S., S. T. Lee and S. W. Huang (1986) Intercropping and modification of irrigation method for the control of diamondback moth. pp. 145-155, In Diamondback moth management: Proceeding of the First International Workshop (eds. N. S. Talekar and T. D. Griggs), Asian Vegetable Research and Development Center, Taiwan.
  16. Talekar, N. S., J. C. Yang and S. T. Lee (1992) Introduction of Diadegma semiclausum to control diamondback moth in Taiwan. pp. 263-270, In Diamondback moth and other crucifer pests: Proceeding of the Second International Workshop (ed. N S. Talekar). Asian Vegetable Research and Development Center, Taiwan.
  17. Talekar, N. S. and A. M. Shelton (1993) Biology, ecology and management of Plutella xylostella (Lepidoptera: Plutellidae). Ann. Rev. Entomol. 38:275-301. https://doi.org/10.1146/annurev.en.38.010193.001423
  18. Waladde, S. M. and M. F. Leutle (2001). Parasitism of Plutella xylostella (Lepidoptera: Plutellidae) : Field and laboratory observations. South African Journal of Plant and Soil. 18:32-37. https://doi.org/10.1080/02571862.2001.10634397
  19. Zhao, J. Z., Y. X. Li, H. L. Collins, L. Gusukuma-Minuto, R. E. L. Mau, G. D. Thompson and A. M. Shelton (2002) Monitoring and characterization of diamondback moth (Lepidoptera: Plutellidae) resistance to spinosad. J. Econ. Entomol. 95:430-436. https://doi.org/10.1603/0022-0493-95.2.430
  20. 권민, 이승환, 최병렬 (2007) 고랭지배추 해충의 천적자원 탐색 및 천적 이용기술 개발. p. 120. 국책기술개발사업 최종보고서, 농촌진흥청.
  21. 권민, 이승환, 이봉훈 (2008) 배추좀나방 실내사육 및 생물적 방제 기술 매뉴얼. p. 35. 고령지농업연구소 및 UNDP사업단(공동발행). 농촌진흥청.
  22. 농과원 (2000) 채소 병해충 진단과 방제. p. 330. 농촌진흥청 농업과학기술원. 아카데미서적, 서울.
  23. 안용준, 조광연 (1992) 살충제 개발을 위한 생물검정법의 확립 - I. 각종 유기용매가 곤충의 독성과 약해 및 화합물의 용해성에 미치는 영향. 한국응용곤충학회지. 31(2):182-189.
  24. 이승찬, 조영식, 김도익 (1993) 배추좀나방(Plutella xylostella L.)의 독성시험방법 비교와 지역별 약제저항성에 관한 연구. 한국응용곤충학회지 32(3):323-329.
  25. 조광연, 안종웅, 안용준, 김진석, 구석진 (1987). 신규 농약 개발을 위한 스크리닝 체계 확립(II). 한국화학연구원. pp. 501-844. 과학기술처.
  26. 최병렬, 이시우, 박형만 (2007) 배추나비고치벌 (Cotesia glomerata L.)에 대한 저독성 약제 및 잔류독성. 한국응용곤충학회지. 46(2):251-259.