KSBB Journal

  • 제26권3호
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  • Pages.229-236
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  • 2011
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  • 1225-7117(pISSN)
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  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
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이타콘산 고생산성 Aspergillus terreus 변이주의 신속 선별을 위한 효율적인 균주 스크리닝 전략 개발

Development of an Efficient Screening Strategy for Rapid Selection of High-yielding Mutants of Itaconic Acid Biosynthesized by Fungal Cells of Aspergillus terreus

  • 투고 : 2011.04.26
  • 심사 : 2011.05.13
  • 발행 : 2011.06.30
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초록

An efficient screening method was developed for rapid selection of a few overproducers of itaconic acid (IA) among the great many mutants derived from mother strains of Aspergillus terreus. For this purpose, an attempt was made to reveal the relationships of the growth rate and sporulation of each mutant on PDA solid medium with its IA productivity in the final liquid production-culture. As a result, it was possible to classify the mutated strains into 5 groups (from [A] to [E] group) according to theirmorphologies (i.e., growth rate and sporulation extent) on the PDA slants. Notably, most of the high-yielding mutants of IA were observed to belong to [A]group which had the properties of the highest growth rate and sporulation among the 5 groups, whereas the mutant groups of [C], [D] and [E] with the contrasting morphological features showed significant reductions in their IA productivities. From these results, it was concluded that the probability of selecting IA overproducing mutants could be remarkably enhanced when the mutated colonies showing faster growth rates are firstly selected on the PDA plate, and then further screening process is performed on the basis of the sporulation extents of the mutants selected. Consequently, through the application of the strategy developed in this study, costs and time involvedin the labor-intensive task of strain improvement could be reduced to a great extent, because the time-consuming liquid culture processes did not need to performed for the unfavorable mutants belonging to the groups other than group [A].

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참고문헌

  1. Tevz, G., M. Bencina, and M. Legisa (2010) Enhancing itaconic acid production by Aspergillus terreus. Appl. Microbiol. Biotechnol. 87: 1657-1664. https://doi.org/10.1007/s00253-010-2642-z
  2. Gyamerah, M. (1995) Factors affecting the growth form of Aspergillus terreus NRRL 1960 in relation to itaconic acid fermentation. Appl. Microbiol. Biotechnol. 44: 356-361. https://doi.org/10.1007/BF00169929
  3. Tsukamoto, S., T. Yoshida,H. Hosono, T. Ohta, and H. Yokosawa (2006) Hexylitaconic acid: A new inhibitor of p53-HDM2 interaction isolated from a marine-derived fungus, Arthrinium spp .. Bioorg. Med. Chem. Lett. 16: 69-71. https://doi.org/10.1016/j.bmcl.2005.09.052
  4. Tong, X., Y. Ma, and Y. Li (2010) Biomass into chemicals: Conversion of sugars to furan derivatives by catalytic processes. Appl. Catal. Gen. 385: 1-13. https://doi.org/10.1016/j.apcata.2010.06.049
  5. Bressler, E. and S. Braun (2000) Conversion of citric acid to itaconic acid in a novel liquid membrane bioreactor. J. Chem. Tech. Biotechnol. 75: 66-72. https://doi.org/10.1002/(SICI)1097-4660(200001)75:1<66::AID-JCTB176>3.0.CO;2-U
  6. Willke, T. and K. Vorlop (2001) Biotechnological production of itaconic acid. Appl. Microbiol. Biotechnol. 56: 289-295. https://doi.org/10.1007/s002530100685
  7. Lin, Y., Y. Li, M. Huang, and Y. Tsai (2004) Intracellular expression of Vitreoscilla hemoglobin in Aspergillus terreus to alleviate the effect of a short break in aeration during culture. Biotechnol. Lett. 26: 1067-1072. https://doi.org/10.1023/B:BILE.0000032964.15178.7c
  8. Flipphi, M., J. Sun, X. Robellet, L. Karaffa, E. Fekete, A. P. Zeng, and C. P. Kubiecek (2009) Biodiversity and evolution of primary carbon metabolism in Aspergillus nidulans and other Aspergillus spp. Fungal Genet BioI. 46 Suppl 1: S19-S44. https://doi.org/10.1016/j.fgb.2008.07.018
  9. Okabe, M., D. Lies, S. Kanamasa, and E. Y. Park (2009) Biotechnological production of itaconic acid and its biosynthesis in Aspergillus ferreus. Appl. Microbiol. Biotechnol. 84: 597-606. https://doi.org/10.1007/s00253-009-2132-3
  10. Shimi, I. R. and M. S. Nour El Dein (1962) Biosynthesis of itaconic acid by Aspergillus terreus. Archiv fur Mikrobiologie. 44: 181-188. https://doi.org/10.1007/BF00408905
  11. Bentley, R. and C. P. Thiessen (1957) Biosynthesis of itaconic acid in Aspergillus terreus. II. Early stages in glucose dissimilation and the role of citrate. J. BioI. Chem. 226: 689-701.
  12. Bentley, R. and C. P. Thiessen (1957) Biosynthesis of itaconic acid in Aspergillus ferreus. III. The properties and reaction mechanism of cis-aconitic acid decarboxylase. J. BioI. Chem. 226: 703-720.
  13. Bonnarme, P., B. Gillet, A. M. Sepulchre, C. Role, J. C. Beloeil, and C. Ducrocq (1995) Itaconate biosynthesis in Aspergillus terreus. J. Bacteriol. 177: 3573-3578.
  14. Dwiarti, L., K. Yamane, H. Yamatani, P. Kahar, and M. Okabe (2002) Purification and characterization of cis-Aconitic acid decarboxylase from Aspergillus terreus TN484-M1. J. Biosci. Bioeng. 94: 29-33 . https://doi.org/10.1016/S1389-1723(02)80112-8
  15. Kanamasa, S., L. Dwiarti, M. Okabe, and E. Y. Park (2008) Cloning and functional characterization of the cis-aconitic acid decarboxylase (CAD) gene from Aspergillus terreus. Appl. Microbiol. Biotechnol. 80: 223-229. https://doi.org/10.1007/s00253-008-1523-1
  16. Archer, D. B., I. F. Connerton, and D. A. MacKenzie (2008) Filamentous fungi for production of food additives and processing aids. Adv. Biochem. Eng. Biotechnol. 111: 99-147.
  17. Yu, J. H. and A. H. Thomas (1999) Manual of Industrial Microbiology and Biotechnology. 2nd ed., pp. 417-434. ASM press, Washington, D. C., USA.
  18. Osada, H. (2010) Introduction of new tools for chemical biology research on microbial metabolites. Biosci. Biotechnol. Biochem. 74: 1135-1140. https://doi.org/10.1271/bbb.100061
  19. Rowlands, R. T. (1984) Industrial strain improvement: Mutagenesis and random screening procedures. Enzyme Microb. Technol. 6: 3-10. https://doi.org/10.1016/0141-0229(84)90070-X
  20. S. Parekh, V. A. Vinci, and R. J. Strobel (2000) Improvement of microbial strains and fermentation processes. Appl. Microbiol. Biotechnol. 54: 287-301. https://doi.org/10.1007/s002530000403
  21. Dmain A. (1971) Overproduction of microbial metabolites and enzymes due to alteration of regulation. Adv. Biochem. Eng. 1: 113-142. https://doi.org/10.1007/BFb0044732
  22. Roze, L. V., A. Chanda, and J. E. Linz (2011) Compartmentalization and molecular traffic in secondary metabolism: A new understanding of established cellular processes. Fungal Genet. Biol. 48: 35-48. https://doi.org/10.1016/j.fgb.2010.05.006
  23. Crueger, W. and A. Crueger(1990)Biotechnology: A Textbook of Industrial Microbiology. 2nd ed., pp. 9-58. Sinauer Associates, Sunderland, MA, USA.
  24. Percival Zhang, Y., M. E. Himmel, and J. R. Mielenz (2006) Outlook for cellulase improvement: Screening and selection strategies. Biotechnol. Adv. 24: 452-481. https://doi.org/10.1016/j.biotechadv.2006.03.003