Mycobiology

The Korean Society of Mycology (한국균학회)
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Heterologous Expression of Endo-1,4-beta-xylanaseA from Phanerochaete chrysosporium in Pichia pastoris

  • Huy, Nguyen Duc (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Thiyagarajan, Saravanakumar (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Son, Yu-Lim (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Park, Seung-Moon (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University)
  • Received : 2011.05.02
  • Accepted : 2011.05.13
  • Published : 2011.06.30
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Abstract

The cDNA of endo-1,$4-{\beta}-xylanaseA$, isolated from Phaenerocheate chrysosporium was expressed in Pichia pastoris. Using either the intrinsic leader peptide of XynA or the ${\alpha}$-factor signal peptide of Saccharomyces cerevisiae, xylanaseA is efficiently secreted into the medium at maximum concentrations of 1,946 U/L and 2,496 U/L, respectively.

Keywords

References

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