• Title/Summary/Keyword: Phanerochaete chsysosporium

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Heterologous Expression of Endo-1,4-beta-xylanaseA from Phanerochaete chrysosporium in Pichia pastoris

  • Huy, Nguyen Duc;Thiyagarajan, Saravanakumar;Son, Yu-Lim;Park, Seung-Moon
    • Mycobiology
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    • v.39 no.2
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    • pp.121-124
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    • 2011
  • The cDNA of endo-1,$4-{\beta}-xylanaseA$, isolated from Phaenerocheate chrysosporium was expressed in Pichia pastoris. Using either the intrinsic leader peptide of XynA or the ${\alpha}$-factor signal peptide of Saccharomyces cerevisiae, xylanaseA is efficiently secreted into the medium at maximum concentrations of 1,946 U/L and 2,496 U/L, respectively.