Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Current status on the development of detection methods for genetically modified plants

유전자변형식물의 검정기술 개발 현황

  • Kim, Jae-Hwan (Department of Food Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University) ;
  • Kim, Young-Rok (Department of Food Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University) ;
  • Kim, Hae-Yeong (Department of Food Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University)
  • 김재환 (경희대학교 식품생명공학과, 생명자원과학연구원) ;
  • 김영록 (경희대학교 식품생명공학과, 생명자원과학연구원) ;
  • 김해영 (경희대학교 식품생명공학과, 생명자원과학연구원)
  • Received : 2011.05.25
  • Accepted : 2011.06.08
  • Published : 2011.06.30
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Abstract

Since the first commercial GM plant, the FlavrSavr tomato, authorized in 1994, more than 140 GM plants were authorized for marketing globally. For the authorization and labelling of GM plants, the detection methods for genes introduced and proteins expressed in GM plants were developed qualitatively and quantitatively. This review presented the detection methods, conventional PCR, multiplex PCR and real-time PCR, for soybean, maize, canola and cotton as the dominant GM plants. Also, microarray assay and nanotechnology as new approaches for detection methods for GM plants were investigated.

1994년 처음으로 GM 토마토인 Flavr Savr가 시장에 나온 이후, 2010년 현재 140여 품목의 GM식물이 전 세계적으로 상업화되었다. GM식물들에 대한 안전성 승인여부의 확인 및 표시제관리를 위하여 이들 GM식물내로 도입된 삽입유전자의 정보를 이용한 검정방법이 도입되었으며, 또한 도입유전자의 발현된 단백질을 분석하기 위하여 정성 및 정량을 위한 면역학적 방법이 도입되었다. 본 총설에서는 국내 외적으로 개발된 콩, 옥수수, 카놀라, 면화 등의 GM식물에 적용된 multiplex PCR, real-time PCR 방법과 최신 개발 중인 microarray, 나노기술 등을 활용한 방법들을 조사하였다.

Keywords

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