Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Anti-inflammatory effect of ethanol extract from Ecklomia cava on gram-positive bacteria(Peptidoglycan)-induced macrophages

그람-양성균(Peptidoglycan)으로 유도한 대식세포에서 감태 에탄올 추출물의 항염증 효과

  • Kang, Ok-Hwa (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University) ;
  • Kim, Sung-Bae (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University) ;
  • Keum, Joon-Ho (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University) ;
  • Mun, Su-Hyun (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University) ;
  • Kim, Yong-Sik (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University) ;
  • An, Byung-Kwan (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University) ;
  • An, Hyeon-Jin (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University) ;
  • Kwon, Dong-Yeul (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University)
  • 강옥화 (원광대학교 한약학과 원광한약연구소) ;
  • 김성배 (원광대학교 한약학과 원광한약연구소) ;
  • 금준호 (원광대학교 한약학과 원광한약연구소) ;
  • 문수현 (원광대학교 한약학과 원광한약연구소) ;
  • 김용식 (원광대학교 한약학과 원광한약연구소) ;
  • 안병관 (원광대학교 한약학과 원광한약연구소) ;
  • 안현진 (원광대학교 한약학과 원광한약연구소) ;
  • 권동렬 (원광대학교 한약학과 원광한약연구소)
  • Received : 2011.05.20
  • Accepted : 2011.06.13
  • Published : 2011.06.30
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Abstract

Objectives : Ecklonia cava is brown alga(Laminariaceae) which grows is sea, it has antioxidant, diarrhea and anticoagulant effect. In this study, the effect of ethanol extract of Ecklonia cava (EC) on peptidoglycan(PGN) -induced NO production in RAW 264.7 cells was investigated. Methods : In the present study, IL-6 production was measured using the enzyme-linked immunosorbent assay(ELISA), prostaglandin $\E_2$($\PGE_2$) production was measured using the EIA kit, and inducible NO synthase(iNOS), cyclooxygenase-2(COX-2), and mitogen-activated protein kinase(MAPK) activation, as determined by western blot analysis and reverse transcription -polymerase chain reaction(RT-PCR). Results : EC inhibited PGN-induced NO and IL-6 production. Consistent with these observations, the protein expression of iNOS and COX-2 were inhibited by EC. Moreover, EC suppressed the phosphorylation of extracellular signal-regulated kinase(ERK) 1/2 in PGN-induced RAW 264.7. Conclusions : These results suggest that EC has inhibitory effects on PGN-induced $\PGE_2$, NO, and IL-6 production, as well as the expressions of iNOS and COX-2 in the murine macrophage. These inhibitory effects occur through blockades on the MAPKs phosphorylation.

Keywords

References

  1. Artan M, Li Y, Karadeniz F, Lee SH, Kim MM, Kim SK. Anti-HIV-l activity of phloroglucinol derivative, 6,6'-bieckol, from Ecklonia cava. Bio org Med Chem. 2006;16(17):7921-6.
  2. Ahn G, Hwang I, Park E, Kim J, Jeon YJ, Lee J, Park JW, Jee Y. Physiological Activities of Hot Water Extracts from Ecklonia cava Kjellman. Mar Biotechnol(NY). 2006;10(3):278-89.
  3. Cho EK, Choi YJ, Physiological Activities of Hot Water Extracts from Ecklonia cava Kjellman. Journal of Life Science. 2010:20(11):1675-82. https://doi.org/10.5352/JLS.2010.20.11.1675
  4. Kang K, Hwang HJ, Hong DH, Park Y, Kim SH, Lee BH, Shin HC. Antioxidant and antiinclammatory activities of vantol, a phlorotannin-rich natural agent derived from Ecklonia cava, and its effect on proteoglycan degradation in cartilage explant culture. Res Commun Mol Pathol Pharmacol. 2004;115-116:77-95.
  5. Medzhitov, R, and C.A. Janeway, Jr, An ancient system of host defense. Curr. Opin. Immunol. 1998;10:12-5. https://doi.org/10.1016/S0952-7915(98)80024-1
  6. Hoffmann JA, Kafatos FC, Janeway CA, Ezekowitz RA, Phylogenetic perspectives in innate immunity. Science 1999;284:1313-8. https://doi.org/10.1126/science.284.5418.1313
  7. Ulevitch RJ, Tobias PS. Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin. Annu. Rev. Immunol. 1995;13:437-57. https://doi.org/10.1146/annurev.iy.13.040195.002253
  8. Nakanishi T, Mukai K, Yumoto H, Hirao K, Hosokawa Y, Matsuo T, Anti-inflammatory effect of catechin on cultured human dental pulp cells affected by bacteria-derived factors. Eur J Oral Sci. 2010;118(2):140-50.
  9. Schleifer KH, Kandler O, Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev. 1972;36:407-77.
  10. Wang Q. Dziarski R, Kirschning CJ, Muzio M, Gupta D. Micrococci and peptidoglycan activate TLR2-> MyD88-> IRAK-> TRAF-> NIK-> IKK-> NF-kappaB signal transduction pathway that induces transcription of interleukin-8. Infect. Immun. 2001;69:2270-6. https://doi.org/10.1128/IAI.69.4.2270-2276.2001
  11. Taylor PR, Brown GD, Herre J, Williams DL, Willment JA, Gordon S. The role of SIGNlRl and the beta-glucan receptor(dectin-l) in the nonopsonic recognition of yeast by specific macrophages. J Immunol. 2004 Jan 15;172(2):1157-62. https://doi.org/10.4049/jimmunol.172.2.1157
  12. McD aniel ML, Kwon G, Hill JR, Marshall CA, Corbett JA. Cytokines and nitric oxide in islet inflammation and diabetes. Proc Soc Exp Biol Med. 1996 Jan;211(1):24-32. Review. https://doi.org/10.3181/00379727-211-43950D
  13. Corbett JA, McDaniel ML. Intraislet release of interleukin 1 inhibits beta cell function by inducing beta cell expression of inducible nitric oxide synthase. J Exp Med. 1990 Feb 1;181(2):559-68.
  14. Heo SJ, Park EJ, Lee KW, Jeon YJ. Antioxidant activities of enzymatic extracts from brown seaweeds. Bioresour Technol. 2000;96:1613-23.
  15. Ahn MJ, Yoon KD, Min SY, Lee JS, Kim JH, Kim TG, Kim SH, Kim NG, Hun H, Kim J. Inhibition of HIV-1 reverse transcriptase and protease by phlorotannins from the brown alga Ecklonia cava. Biol Pharm Bull 2004;27:544-7. https://doi.org/10.1248/bpb.27.544
  16. Kim JA, Lee JM, Shin DB, Lee KH. The antioxidant activity and tyrosinase inhibitory activity of phlorotannins in Ecklonia cava. Food Sci Biotechnol. 2004;13:476-80.
  17. Kim SK, Lee DY, Jung WK, Kim JH, Choi I, Park SG, Seo SK, Lee SW, Lee CM, Yea SS, Choi YH, Choi IW. Effects of Ecklonia cava ethanolic extracts on airway hyperresponsiveness and inflammation in a murine asthma model: role of suppressor of cytokine signaling. Biomed Pharmacother. 2008;62:289-96. https://doi.org/10.1016/j.biopha.2007.07.009
  18. Le QT, Li Y, Qian ZJ, Kim MM, Kim SK. Inhibitory effects of polyphenols isolated from marine alga Ecklonia cava on histamine release. Process Biochem. 2009;44:168-76. https://doi.org/10.1016/j.procbio.2008.10.002
  19. Shim SY, To LQ, Lee SH, Kim SK. Ecklonia cava extract suppresses the high-affinity IgE receptor. FceR I expression. Food Chem Toxicol. 2009;47:550-60.
  20. Ahn GN, Hwang IS, Park EJ, Kim JH, Jeon YJ, Lee JH, Park JW, Jee YH. Immunomodulaltory effects of enzymatic extract from Ecklonia cava on murine splenocytes. Mar Biotechnol. 2008;10:278-89. https://doi.org/10.1007/s10126-007-9062-9
  21. Koyanagi M, Egashira K, Kubo-Inoue M, Usui M, Kitamoto S, Tomita H, Shimokawa H, Takeshita A. Role of transforming growth factor-betal in cardiovascular inflammatory changes induced by chronic inhibition of nitric oxide synthesis. Hypertension. 2000;35(1 Pt 1):86-90. https://doi.org/10.1161/01.HYP.35.1.86
  22. Evans CH. Nitric oxide: what role does it play in inflammation and tissue destruction? Agents Actions Suppl. 1990;47:107-16.
  23. Kim KW, Ha KT, Park CS, Jin UH, Chang HW, Lee IS, Kim CH. Polygonum cuspidatum, compared with baicalin and berberine. inhibits inducible nitric oxide synthase and cyclooxygenase-2 gene expressions in RAW 264.7 macrophages. Vascul Pharmacol. 2007;47(2-3):99-107. https://doi.org/10.1016/j.vph.2007.04.007
  24. Forstermann U, Kleinert H. Nitric oxide synthase: expression and expressional control of the three isoforms. Naunyn Schmiedebergs Arch Pharmacol. 1990;352(4):351-64.
  25. Masferrer JL, Zweifel BS, Manning PT, Hauser SD, Leahy KM, Smith WG, Isakson PC, Seibert K. Selective inhibition of inducible cyclooxygenase 2 in vivo is anti-inflammatory and nonulcerogenic. Proc Natl Acad Sci USA. 1994;91(8):3228-32. https://doi.org/10.1073/pnas.91.8.3228
  26. Ahn KS; Noh EJ; Zhao HL, Jung SH, Kang SM, Kim YS. Inhibition of inducible nitric oxide synthase and cyclooxygenase II by Platycodon grandiflorum saponins via suppression of nuclear factor-kappaB activation in RAM 264.7 cells. Life Sci. 2005;76:2315-232. https://doi.org/10.1016/j.lfs.2004.10.042
  27. Chen C, Chen YH, Lin WW. Involvement of p38 mitogen-activated protein kinase in lipopolysaccharide-induced iNOS and COX-2 expression in J774 macrophage. Immunology 1999;97:124-9. https://doi.org/10.1046/j.1365-2567.1999.00747.x
  28. Chen Y, Yang L, Lee TJ, Oroxylin A inhibition of lipopolysaccharide-induced iNOS and COX-2 gene expression via Suppression of nuclear factor-kappaB activation. Biochem Pharmacol 2000;59:1445-57. https://doi.org/10.1016/S0006-2952(00)00255-0