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http://dx.doi.org/10.14374/HFS.2011.19.1.195

Anti-inflammatory effect of ethanol extract from Ecklomia cava on gram-positive bacteria(Peptidoglycan)-induced macrophages  

Kang, Ok-Hwa (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University)
Kim, Sung-Bae (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University)
Keum, Joon-Ho (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University)
Mun, Su-Hyun (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University)
Kim, Yong-Sik (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University)
An, Byung-Kwan (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University)
An, Hyeon-Jin (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University)
Kwon, Dong-Yeul (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Won-Kwang University)
Publication Information
Herbal Formula Science / v.19, no.1, 2011 , pp. 195-205 More about this Journal
Abstract
Objectives : Ecklonia cava is brown alga(Laminariaceae) which grows is sea, it has antioxidant, diarrhea and anticoagulant effect. In this study, the effect of ethanol extract of Ecklonia cava (EC) on peptidoglycan(PGN) -induced NO production in RAW 264.7 cells was investigated. Methods : In the present study, IL-6 production was measured using the enzyme-linked immunosorbent assay(ELISA), prostaglandin $\E_2$($\PGE_2$) production was measured using the EIA kit, and inducible NO synthase(iNOS), cyclooxygenase-2(COX-2), and mitogen-activated protein kinase(MAPK) activation, as determined by western blot analysis and reverse transcription -polymerase chain reaction(RT-PCR). Results : EC inhibited PGN-induced NO and IL-6 production. Consistent with these observations, the protein expression of iNOS and COX-2 were inhibited by EC. Moreover, EC suppressed the phosphorylation of extracellular signal-regulated kinase(ERK) 1/2 in PGN-induced RAW 264.7. Conclusions : These results suggest that EC has inhibitory effects on PGN-induced $\PGE_2$, NO, and IL-6 production, as well as the expressions of iNOS and COX-2 in the murine macrophage. These inhibitory effects occur through blockades on the MAPKs phosphorylation.
Keywords
Ethanol extract of Ecklonia cava(EC); gram-positive bacteria(Peptidoglycan); anti-inflammation; Macrophages;
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