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Estimation of nuclear DNA content of various bamboo and rattan species

  • Kumar, Prakash P. (Department of Biological Sciences, National University of Singapore) ;
  • Turner, Ian M. (Department of Biological Sciences, National University of Singapore) ;
  • Rao, A. Nagaraja (Department of Biological Sciences, National University of Singapore) ;
  • Arumuganathan, K. (Flow Cytometry and Imaging Core Laboratory, Benaroya Research Institute at Virginia Mason)
  • Received : 2011.05.11
  • Accepted : 2011.05.23
  • Published : 2011.10.31

Abstract

We determined the nuclear DNA content (genome size) of over 35 accessions each of bamboo and rattan species from Southeast Asia. The 2C DNA per nucleus was quantified by flow cytometry. The fluorescence of nuclei isolated from the leaves and stained with propidium iodide was measured. The genome size of the bamboo species examined was between 2.5 and 5.9 pg DNA per 2C nucleus. The genome size of the rattan species examined ranged from 1.8 to 10.5 pg DNA per 2C nucleus. This information will be useful for scientists working in diverse areas of plant biology such as biotechnology, biodiversity, genome analysis, plant breeding, physiology and molecular biology. Such data may be utilized to attempt to correlate the genome size with the ploidy status of bamboo species in cases where ploidy status has been reported.

Keywords

Acknowledgement

Supported by : IPGRI

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Cited by

  1. Genetics and genomics of moso bamboo (Phyllostachys edulis): Current status, future challenges, and biotechnological opportunities toward a sustainable bamboo industry vol.9, pp.4, 2011, https://doi.org/10.1002/fes3.229