• 한국가금학회지
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• 제38권1호
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• pp.37-43
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• 2011

본 연구는 맹장 결찰계를 이용하여서 [15N]urea의 이용성을 조사하였다. 질소의 balance 및 이용률은 맹장 결찰 수술에 의해서 높아지는 경향이 인정되었다. 이는 맹장 결찰 수술로 인한 요산 배설량의 유의한 감소로 인한 결과였다(사료 및 요소 유래 질소 이용률, P<0.01). 닭에 있어서 맹장 결찰 수술은 사료 유래 ammonia 배설량은 증가(P<0.01), 요소 유래 ammonia배설량은 감소(P<0.01)시켰다. 요소 유래, 요소질소의 배설량은 맹장 결찰 수술에 의해서 유의하게 증가하였지만(P<0.01), 맹장 결찰계도 5% 단백질 사료와 같이 급여한 요소를 51.6%정도 이용하는 것으로 나타났다. 결론적으로 닭에 있어서 단백질 수준이 결핍된 사료와 같이 급여된 요소의 이용에 있어서 맹장은 유의한 역할을 한다고 할 수 있다. 하지만 질소의 이용에 있어서는 반드시 긍정적인 역할만을 한다고는 결론내릴 수 없다.

• Asian-Australasian Journal of Animal Sciences
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• 제31권10호
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• pp.1627-1634
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• 2018

Objective: The study aimed to evaluate the effect of replacing wheat bran for cactus cladodes plus urea (0%, 25%, 50%, 75%, and 100%) on the intake of nutrients, nitrogen balance, microbial protein synthesis, and rumen fermentation for steers. Methods: Five crossbred steers (1/2 Holstein-Zebu), with rumen cannula and an average body weight of $180{\pm}5.3kg$, were assigned to a $5{\times}5$ Latin square design. Dietary treatments consisted of the replacement of the total of wheat bran in basal diet by cactus cladodes using the following proportions: 0% for basal diet, 25%, 50%, 75%, and 100% cactus cladodes replacing wheat bran. Urea was added to the diets to adjust the crude protein (CP) content to 130 g/kg dry matter. Results: Maximum dry matter intake (5.73 kg/d) and maximum nitrogen balance (103 g/d) were estimated for 54.6% and 70.8% replacement levels of wheat bran. The maximum microbial protein production (44.6 g/d) was obtained at a replacement level of 49.7%, and a medium value (125 g CP mic/kg total digestible nutrients) of microbial protein efficiency was observed. The rumen pH increased linearly according to cactus cladodes inclusion, while the ammonia nitrogen medium value was 24.5 mg/dL. Conclusion: The replacement of 55% wheat bran for cactus cladodes plus urea in the diet of crossbred steers is recommended.

• Asian-Australasian Journal of Animal Sciences
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• 제25권5호
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• pp.659-664
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• 2012

The present experiment aimed to evaluate the effect of HMBi on the production performance and metabolism in dairy cows. Thirty multiparous Holstein dairy cows under similar conditions were randomly assigned to three dietary treatments; i) Control, a basal diet; ii) T1, a basal diet plus HMBi (0 g prepartum and 18 g postpartum); and iii) T2, a basal diet plus HMBi (10 g prepartum and 18 g postpartum). Treatments were initiated 21 d before expected calving and continued through 91 d postpartum. HMBi was top-dressed onto the total mixed ration of each cow. Treatments did not affect dry matter intake, plasma urea nitrogen, peak milk yield, days to peak milk yield, nonesterified fatty acid, glutamate pyruvate transaminase, glutamic oxalaetic transaminase, milk fat content, milk protein content, milk lactose content, and milk solid non-fat content. The milk composition yields were increased by the HMBi-supplemented treatment. The T1 and T2 treatments increased the yields of 4% fat-corrected milk yield, milk fat, milk protein, and milk lactose compared with the control. Although there was no difference in the milk composition of the control and T2-treated cows, the T2-treated cows exhibited higher milk fat yield (increased by 74 g/d), lower milk urea nitrogen (reduced by 3.41%), and plasma ${\beta}$-hydroxy butyrate than the control cows. The results indicate that HMBi supplementation to diet has beneficial effects, and that there is no difference between supplementation at prepartum and starting only at parturition.

• 대한본초학회지
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• 제33권5호
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• pp.89-103
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• 2018

Objectives : This study is aimed to compare the changes in Antioxidative capacity of Liriopis Tuber by steaming process and to compare the effects in hyperlipidemia induced rats fed high cholesterol diet between Simvastatin and Liriopis Tuber by steaming process. Methods : The SD rats were divided into six groups: normal diet (Nor), high cholesterol diet (Veh), high cholesterol diet plus Simvastatin 5 mg/kg (Sim), high cholesterol diet plus LT0 extract 200 mg/kg (LT0), high cholesterol diet plus LT6 extract 200 mg/kg (LT6) and high cholesterol diet plus LT9 extract 200 mg/kg (LT9). We compared the total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL), low density lipoprotein cholesterol (LDL) contents and reactive oxygen species (ROS) from each serums. Protein expression in liver tissues related to antioxidant and cholesterol was analyzed. Results : The Antioxidant activity of Liriopis Tuber increased by steaming process. In vivo, TC, TG, LDL-c, atherogenic index (AI) and cardiac risk factor (CRF) decreased and HDL-c increased with increasing steaming frequency. aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine, and blood urea nitrogen (BUN) decreased with increasing steaming frequency. ROS decreased only in LT9, and SOD, catalase and glutathione peroxidase (GPx) increased with increasing steaming frequency. phospho-AMP-activated protein kinase (p-AMPK) increased and sterol regulatory element-binding protein 2 (SREBP-2), Phospho-Acetyl-CoA Carboxylase (p-ACC) and HMG-CoA reductase (HMGCR) decreased with increasing steaming frequency. Liver staining showed a decrease in hepatic fat accumulation of LT9. LT9 showed significant results in all experiments. Conclusions : LT9 showed significance of anti-lipid effect and improved fatty liver of hyperlipemia induced rats fed on high cholesterol diet, In conclusion, LP9 can be effectively used for the treatment of hyperlipidemia.

• Asian-Australasian Journal of Animal Sciences
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• 제11권1호
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• pp.30-34
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• 1998

To compare response of feeding concentrate mixtere and wheat straw (Group I) with either urea molasses liquid diet (UMLD) as sole ration (group II) or UMLD (similar to protein equivalent of concentrate mixture) plus wheat straw (group III) on intake and utilisation of nutrients and overall performance, twelve crossbred adult male cattle (Holstein Friesian ${\times}$ Hariana) aged about 2.5 years and weighing 342 kg were randomly allotted into three equal groups following completely randomised design and fed respective diets for a period of 60 days. Thereafter, a metabolism trial of seven days duration was conducted to assess nutrient utilisation and nitrogen balance. Animals fed UMLD as sole ration consumed significantly (p < 0.01) less amount of most of the nutrients as compared to other two groups, except crude protein, intake of which was higher in this group but it was comparable between II and III and III and I. On the other hand, digestibilities of nutrients were higher (p < 0.01) in group II, though it was comparable between other two groups, except ADF, the digestibility of which was lower in group II. Inspite of positive nitrogen balance in all the three groups, being significantly (p < 0.01) higher in group I, animals of group II and III lost their body weight especially to the utmost extent in group II, although the amount of energy (TDN) intake were similar statistically. Results indicate that during a scarcity period and economic compulsions, feeding of UMLD can be practised to replace only concentrate mixture from the maintenance ration of adult crossbred cattle for shorter duration as roughage part seems to be essential for the normal functioning of the rumen microbes and overall performance of the animals.

• Asian-Australasian Journal of Animal Sciences
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• 제1권2호
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• pp.107-114
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• 1988

Six steers of each breed type, Hereford ($H{\times}H$), Brahman ($B{\times}B$) and Brahman $\times$ Hereford ($B{\times}H$) were ranked on liveweight and allocated to three treatments, basal hay diet (Basal), Basal plus 1 kg/head/day of cottonseed meal (Basal + CSM) and Basal plus 1 kg/head/day of formaldehyde-treated sunflower meal, $Norpro^{(R)}$ (Basal + NPO). The hay was made from a pasture based predominantly on carpet grass (Axonopus affinus) growing in subtropical New South Wales, and had an estimated organic matter digestibility of 52% and a nitrogen (N) content of 7.8 g/kg dry matter (DM). The steers were accustomed to the Basal diet over 15 days and supplements offered over 42 days. Intake of the basal hay diet by steers was not increased by supplementation. When intakes were adjusted for differences between breed types in liveweight the $B{\times}H$ steers ate 25% (P<0.01) more hay than $H{\times}H$ steers (6.3 V 5.0 kg/head/d) and $B{\times}B$ steers ate 8% less hay than $H{\times}H$ steers. Supplementation significantly (P<0.01) increased liveweight gain during the experiment, being ($g/head/d\;{\pm}\;s.e.d.$) 290, 770 and $795{\pm}118$ respectively for Basal, Basal + NPO and Basal + CSM. There was no difference between supplements in the liveweight gain of steers nor between steers of different genotypes. However, there was a significant interaction (P<0.01) between breeds and treatments such that $B{\times}B$ steers gained most on the basal diet but least of the breed types when supplemented. The estimated non-degradeable fraction of N in the protein meals was 58.5 and 44.5%, respectively for NPO and CSM. Both meals increased (P<0.01) plasma urea N and rumen ammonia N concentrations.

• Asian-Australasian Journal of Animal Sciences
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• 제14권5호
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• pp.631-639
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• 2001

Two experiments were carried out concerning the effects of urea-molasses cake (UMC) and its separate components as supplements on rumen environment, in sacco feed degradability and intake of swamp buffaloes fed rice straw, grasses or a mixture of grasses and rice straw. Experiment 1 was a change-over design with 4 animals and 6 treatments. The buffaloes were fed rice straw ad libitum, and the experimental treatments were: no supplementation (R); 700 g of the complete urea-molasses cake (RUMC); 53.2 g urea (RU); 276 g rice bran and 52.5 coconut meal (RRC); 26.6 g salt, 26.6 g bone meal and 2.1 g trace minerals (RMi); and 25 g molasses (RMo). Experiment 2 was a Latin square design with four diets and four animals. The treatments were: rice straw ad libitum and mixed grass (RG) at 2.5 g dry matter per kg live weight (LW); RG plus 700 g urea-molasses cake (RGUMC); mixed grass ad libitum (G); and G plus 700 g cake (GUMC). In both experiments the supplements were fed once daily. In Exp. 1 although the rumen pH was significantly different (p<0.05) among diets, it varied only from 6.90 to 7.06. The ruminal ammonia was also significantly (p<0.05) different among the diets with RUMC significantly higher than R. Total bacterial and protozoal counts were significantly (p<0.05) higher for the RUMC, RU, RMo and RRC diets. Total feed and rice straw intakes were highest for RUMC (p<0.05) and lowest for the RMi and RMo diets, but in sacco degradability of four different roughages were not significantly different among diets. In Exp. 2, rumen pHs of the diets differed significantly and (p<0.01) ranged from 7.04 - 7.19. Ruminal $NH_3-N$ concentrations (mg/100 ml) were also significantly different (p<0.05), and higher for the RGUMC, G and GUMC diets. The total counts of bacteria and protozoa were significantly (p<0.05) higher for the RGUMC, G and GUMC diets. The total feed intake and roughage intake were significantly (p<0.05) higher for the RGUMC, G and GUMC diets compared to the RG diet. Correspondingly, LW changes also differed among treatments (p=0.06). It was concluded that there were significant increases in rumen $NH_3-N$ concentration, microbial populations and feed intake in the buffaloes by UMC supplementation, whereas the significant difference in in sacco DM degradation was not found by any type of supplementation. There seemed to be a need of a combination of urea, molasses, minerals and other protein nitrogen sources to enhance rice straw intake. Adding grass to the rice straw diet at 0.25% LW (DM) should also be considered to maintain buffalo rumen function and production with UMC supplementation, when rice straw is the main roughage.

• Asian-Australasian Journal of Animal Sciences
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• 제22권5호
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• pp.659-666
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• 2009

Metabolizable protein (MP) supply and amino acid balance in the intestine were manipulated through selection of highly digestible rumen-undegradable protein (RUP) sources and protected methionine (Met) supplementation. Four ruminallycannulated, multiparous Holstein cows averaging 193${\pm}$13 days in milk were used in a 4${\times}$4 Latin square design to assess N utilization and milk production responses to changes in RUP level, post-ruminal RUP digestibility and protected Met supplementation. Treatments were A) 14.0% crude protein (CP), 8.0% rumen degradable protein (RDP) and 6.0% RUP of low intestinal digestibility (HiRUP-LoDRUP); B) 14.1% CP, 8.1% RDP and 6.0% RUP of high intestinal digestibility (HiRUP-HiDRUP); C) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility (LoRUP-HiDRUP), and D) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility plus rumen escape sources of Met (LoRUP-HiDRUP+Met). Experimental diets were formulated to have similar concentrations of RDP, net energy of lactation ($NE_L$), neutral detergent fiber (NDF), acid detergent fiber (ADF), calcium, phosphorus and ether extract using the NRC model (2001). Results showed that dry matter intake (DMI), production of milk fat and protein were similar among treatments. Milk production was similar for diet HiRUP-LoDRUP, HiRUP-HiDRUP and LoRUP-HiDRUP+Met, and significantly higher than diet LoRUP-HiDRUP. Milk fat and protein percentage were higher for cows receiving HiDRUP treatments, with the greatest increases in the diet LoRUP-HiDRUP+Met. There was no significant change in ruminal pH, $NH_3g-N$ and volatile fatty acid (VFA) concentration among all treatments. Apparent digestibility of dry matter (DM), CP, NDF and ADF and estimated bacterial CP synthesis were similar for all treatments. Nitrogen intakes, blood and milk urea-N concentrations were significantly higher for cows receiving HiRUP diets. Urine volume and total urinary N excretion were significantly lowered by LoRUP diets. Lowering dietary RUP level while supplementing the highly digestible RUP source with rumen escape sources of Met resulted in similar milk production, maximal milk fat and protein concentration and maximum N efficiency, indicating that post-ruminal digestibility of RUP and amino acid balance in the small intestine can be more important than total RUP supplementation.

• 한국식품영양과학회지
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• 제31권5호
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• pp.808-813
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• 2002

식이섬유소원인 셀룰로스, 차전자피 및 펙틴의 단독 또는 혼합 급여에 의한 Sprague-Dawley계 수컷 흰쥐의 지질 농도 및 임상생화학적 지표 효소에 미치는 영향을 검토하였다. 실험 식이군은 식이 섬유소원에 따라 셀룰로스 투여군, 차전자피 투여군, 펙틴 투여군 및 차전자피와 펙틴 혼합 투여군으로 나누었으며, 이들식이 섬유소원은반합성 식이에 10%(w/w) 수준으로 첨가하여 6주간 자유급여 시켰다. 체중 증가량, 식이 섭취량, 각 조직의 상대적 중량은 각 실험군간에 유의한 차이는 없었다. 차전자피 투여군 및 펙틴 투여군에서 혈청 중성지질 및 인지질 농도는 셀룰로스 투여군 및 차전자피와 펙틴 혼합 투여군보다 통계상 유의하게 낮게 나타났다. 혈청 총 콜레스테롤 농도는 차전자피와 펙틴 혼합 투여군이 다른 식이 투여군보다 감소경향을 나타내었다. 그러나 간장 중성지질 및 인지질 농도는 혈청 지질 농도와는 반대로 차전자피 투여군 및 펙틴 투여군보다 셀룰로스 투여군 및 차전자피와 펙틴 혼합 투여군에서 낮게 나타났다. 차전자피 투여군의 혈청 임상생 화학적 지표 효소 glutamic pyruvic transaminase, glutamic oxaloacetic transaminase, alkaline phosphatase, lactate do hydrogenase 활성과 총단백질, albumin, blood urea nitrogen은 다른 식이 투여군보다 낮게 나타났다 이상의 결과로부터 식이 섬유소인 차전자피 급여는 혈청 임상생화학적 지표에 크게 영향을 미치지 않으면서 중성지질 농도를 낮추는 작용이 있는 것으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제5권2호
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• pp.267-274
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• 1992

In order to study the relationships between the contents of urinary nitrogenous compounds and energy utilization of bird, the sum of nitrogen contents of uric acid, ammonia, creatine and urea voided in excreta was estimated as the urinary nitrogen (UN) in 13-33 day-old fed or fasted White Leghorn male chicks. Energy retention and heat production of birds were determined by comparative slaughter studies. 2.75 mg of endogenous urinary nitrogen (EUn) and 2.19 mg of uric acid was excreted constantly per kJ heat production in fasted bird. One mg of UN was proportionated to 32.26 J (r = 0.999, n = 8) of the urinary energy (UE) in fed and 32.97 J (r = 0.9998, n = 8) of the endogenous urinary energy (UEn) in the fasted bird. Also relationships between 1 mg of uric acid and 38.95 J of UE (r = 0.998, n = 8) or 38.97 J of UEe (r = 0.996, n = 8) were significant (p<0.01). The EUn (r = 0.997, n = 4), uric acid (r = 0.995, n = 4) and metabolic fecal energy (FEm) plus UEe (r = 0.961, n = 4) were increased with the increase of body weight (g/bird). Metabolic fecal nitrogen (MFn) or energy (FEm), EUn and UEe per unit diet were not influenced by the age of day or body weight. The results indicated that energy and protein utilization of bird can be approximated by the relationships among urinary nitrogen, urinary energy, uric acid content in excreta and body weight of bird.