Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지
DOI QR코드

DOI QR Code

Development of serodiagnostic surface plasmon resonance imaging assay for the detection of antibodies to porcine circovirus type 2

  • Park, Chul (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Kim, Bum-Seok (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Kim, Yong-Hwan (Department of Veterinary Research, Gwangju Metropolitan City Institute of Health & Environment) ;
  • Cho, Ho-Seong (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
  • Received : 2010.01.16
  • Accepted : 2010.03.25
  • Published : 2011.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

A surface plasmon resonance imaging (SPRI) assay was developed for measuring porcine circovirus type 2 (PCV2) antibody using a recombinant capsid protein as an antigen. The diagnostic potential of SPRI for detecting antibodies to the PCV2 capsid protein was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) using 70 pig serum samples taken from 6 pig farms. There was a strong positive correlation between the SPRI and ELISA (n = 70, r = 0.911, P<0.01). Therefore, this recombinant capsid protein can be used as an antigen for serological studies, and the SPRI, a label-free and high-throughput method, is expected to be a valuable tool in the serodiagnosis of PCV2 infection.

Keywords

References

  1. Blanchard P, Mahe D, Cariolet R, Truong C, Le Dimna M, Arnauld C, Rose N, Eveno E, Albina E, Madec F, Jestin A. 2003. An ORF2 protein-based ELISA for porcine circovirus type 2 antibodies in post-weaning multisystemic wasting syndrome. Vet Microbiol 94(1): 183-194. https://doi.org/10.1016/S0378-1135(03)00131-7
  2. Cho HS, Park NY. 2006. Serodiagnostic comparison between two methods, ELISA and surface plasmon resonance for the detection of antibodies of classical swine fever. J Vet Med Sci 68(12): 1327-1329. https://doi.org/10.1292/jvms.68.1327
  3. Cho HS, Park SJ, Park NY. 2006. Serodiagnostic comparison of enzyme-linked immunosorbent assay and surface plasmon resonance for the detection of antibody to porcine circovirus type 2. Can J Vet Res 70(4): 263-268.
  4. Gillespie J, Opriessnig T, Meng XJ, Pelzer K, Buechner-Maxwell V. 2009. Porcine circovirus type 2 and porcine circovirus- associated disease. J Vet Intern Med 23(6): 1151- 1163. https://doi.org/10.1111/j.1939-1676.2009.0389.x
  5. Kim TJ, Cho HS, Park NY, Lee JI. 2006. Serodiagnostic comparison between two methods, ELISA and surface plasmon resonance for the detection of antibody titers of Mycoplasma hyopneumoniae. J Vet Med B Infect Dis Vet Public Health 53(2): 87-90. https://doi.org/10.1111/j.1439-0450.2006.00917.x
  6. Liu Q, Wang L, Willson P, Babiuk LA. 2000. Quantitative, competitive PCR analysis of porcine circovirus DNA in serum from pigs with postweaning multisystemic wasting syndrome. J Clin Microbiol 38(9): 3474-3477.
  7. Mahe D, Blanchard P, Truong C, Arnauld C, Le Cann P, Cariolet R, Madec F, Albina E, Jestin A. 2000. Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes. J Gen Virol 81(7): 1815-1824. https://doi.org/10.1099/0022-1317-81-7-1815
  8. Meerts P, Misinzo G, Lefebvre D, Nielsen J, Botner A, Kristensen CS, Nauwynck HJ. 2006. Correlation between the presence of neutralizing antibodies against porcine circovirus 2 (PCV2) and protection against replication of the virus and development of PCV2-associated disease. BMC Vet Res 2: 6. https://doi.org/10.1186/1746-6148-2-6
  9. Nawagitgul P, Morozov I, Bolin SR, Harms PA, Sorden SD, Paul PS. 2000. Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein. J Gen Virol 81(9): 2281-2287.
  10. Oh K, Lee S, Seo J, Lee D, Kim T. 2010. Rapid serodiagnosis with the use of surface plasmon resonance imaging for the detection of antibodies against major surface protein A of Mycoplasma synoviae in chickens. Can J Vet Res 74(1): 71-74.
  11. Opriessnig T, Meng XJ, Halbur PG. 2007. Porcine circovirus type 2-associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies. J Vet Diagn Invest 19(6): 591-615. https://doi.org/10.1177/104063870701900601