Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
권호 표지
DOI QR코드

DOI QR Code

Sensitivity Enhancement of Polydiacetylene Vesicles through Control of Particle Size and Polymerization Temperature

입자크기와 중합온도 제어를 통한 폴리다이아세틸렌의 센싱감도 향상

  • Lee, Gil Sun (Department of Chemical and Biological Engineering, Korea University) ;
  • Oh, Jae Ho (Department of Chemical and Biological Engineering, Korea University) ;
  • Ahn, Dong June (Department of Chemical and Biological Engineering, Korea University)
  • 이길선 (고려대학교 화공생명공학과) ;
  • 오재호 (고려대학교 화공생명공학과) ;
  • 안동준 (고려대학교 화공생명공학과)
  • Published : 2011.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

Many studies on polydiacetylene(PDA) have been investigated to apply to chemical and biological sensors due to their unique optical properties of color change from blue to red and fluorescence change from non-fluorescence to red fluorescence. Especially, high sensitivity against specific molecules is very important to apply polydiacetylenes to various sensors. In this study, we examined the effect of sensitivity enhancement of 10,12-pentacosadynoic acid(PCDA) vesicles in detection ${\alpha}$-cyclodextrin(CD) according to control of vesicle size by filters with different pore sizes and polymerization temperature. Colorimetric response(CR) was calculated using visible spectrometer. In order to investigate the effect of vesicle size on sensitivity of PDA vesicles, two PCDA vesicles were filtered without filtration and with 0.22 ${\mu}m$ filter. The two PCDA vesicles were polymerized at $25^{\circ}C$ and were incubated with ${\alpha}$-CD(5 mM) for 30 min. The CRs of the former and latter vesicles were 31.4% and 74.0%, respectively. Then, two PCDA vesicles filtered with 0.22 ${\mu}m$ filter were polymerized at $25^{\circ}C$ and $5^{\circ}C$ and were reacted with ${\alpha}$-CD(5 mM) for 30 min to examine the effect of polymerization temperature. The CRs of the former and latter vesicles were 74.0 and 99.2%, respectively. This suggests that vesicle sizes and polymerization temperature are key factors in enhancing the sensitivity of PDA vesicles. In addition, these results are expected to be useful to apply the PDA vesicles as biosensors to detect DNA, protein, and cells.

폴리다이아세틸렌(polydiacetylene: PDA)은 독특한 광학적 특성, 즉 외부자극에 의하여 파란색에서 빨간색으로 색상이 변화하는 동시에 형광이 없던 상태에서 자가형광을 발현하는 특성 때문에 화학, 바이오센서로써 응용하기 위한 많은 연구들이 진행되어 왔다. 특히, 센서의 성능에서 감지하고자 하는 물질에 대한 우수한 민감도는 매우 중요하다. 본 연구에서는 다양한 필터 사이즈를 이용하여 10,12-pentacosadynoic acid(PCDA) 베시클의 크기를 조절함과 동시에 중합온도를 조절하여 ${\alpha}$-사이클로텍스트린(CD)을 검출하여 두 가지 효과가 민감도 향상에 어떤 영향을 미치는지 조사하였다. 필터를 사용하지 않은 베시클과 0.22 ${\mu}m$로 필터한 베시클을 $25^{\circ}C$에서 고분자한 후에 ${\alpha}$-CD(5 mM)와 30분 반응하였을 때 색전이 정도(colorimetric response, CR)가 31.4%에서 74.0%로 증가하였다. 또한, 0.22 ${\mu}m$로 필터한 베시클을 $25^{\circ}C$와 $5^{\circ}C$에서 고분자한 후에 ${\alpha}$-CD(5 mM)와 30분 반응하였을 때 CR값이 74.0%에서 99.2%로 증가하였다. 이는 폴리다이아세틸렌의 크기와 고분자시 온도를 조절함으로써 민감도를 크게 증가시킬 수 있음을 보여준다. 또한, 폴리다이이아세틸렌은 감도 향상이 매우 중요한 바이오물질을 검출하는데 적용될 수 있을 것이다.

Keywords

References

  1. Fendler, J. H., "Atomic and Molecular Clusters in Membrane Mimetic Chemistry," Chem. Rev., 87, 877-899(1987). https://doi.org/10.1021/cr00081a002
  2. Swager, T. M., "The Molecular Wire Approach to Sensory Signal Amplification," Acc. Chem. Res., 31, 201-207(1998). https://doi.org/10.1021/ar9600502
  3. Ahn, D. J. and Kim, J.-M., "Fluorogenic Polydiacetylene Supramolecules: Immobilization, Micropatterning, and Application to Label-Free Chemosensors," Acc. Chem. Res., 41, 805-816(2008). https://doi.org/10.1021/ar7002489
  4. Lee, K., Povlich, L. K. and Kim, J., "Recent Advnaces in Fluorescent and Colorimetric Conjugated Polymer-based Biosensors," Analyst, 135, 2179-2189(2010). https://doi.org/10.1039/c0an00239a
  5. Exarhos, G. J., Risen, W. M. and Bauhman, R. H., "Resonance Raman Study of the Thermochromic Phase Transition of a Polydiacetylene," J. Am. Chem. Soc., 98, 481-487(1976). https://doi.org/10.1021/ja00418a026
  6. Chu, B. and Xu, R., "Chromatic Transition of Polydiacetylene in Solution," Acc. Chem. Res., 24, 384-389(1991). https://doi.org/10.1021/ar00012a005
  7. Cheng, Q., Peng, T. and Stevens, R. C., "Signaling of Escherichia Coli Enterotoxin on Supramolecular Redox Bilayer Liposomes," J. Am. Chem. Soc., 121, 6767-6768(1999). https://doi.org/10.1021/ja991234p
  8. Kim, K.-W., Choi, H., Lee, G. S., Oh, M.-K. and D. J. Ahn, D. J., "Micro-patterned Polydiacetylene Vesicle Chips for Detecting Protein- Protein Interactions," Macromol. Res., 14, 483-485(2006). https://doi.org/10.1007/BF03219115
  9. Lee, S. W., Kang, C. D., Yang, D. H., Lee, J.-S., Kim, J.-M., Ahn, D. J. and Sim, S. J., "The Development of a Generic Bioanalytical Matrix Using Polydiacetylenes," Adv. Funct. Mater., 17, 2038-2044(2007). https://doi.org/10.1002/adfm.200600398
  10. Kim, K.-W., Choi, H., Lee, G. S., Ahn, D. J. and Oh, M.-K., "Effect of Phospholipid Insertion on Arrayed Polydiacetylene Biosensors," Colloid Surfaces B:Biointerfaces, 66, 213-217(2008). https://doi.org/10.1016/j.colsurfb.2008.06.020
  11. Wang, C., Ma, Z. and Su, Z., "Facile Method to Detect Oligonucleotides with Functionalized Polydiacetylene Vesicles," Sensor Actuat. B: Chem., 113, 510-515(2006). https://doi.org/10.1016/j.snb.2005.03.106
  12. Okada, S., Peng, S., Spevak, W. and Charych, D., "Color and Chromism of Polydiacetylene Vesicles," Acc. Chem. Res., 31, 229-239(1998). https://doi.org/10.1021/ar970063v
  13. Ma, Z., Li, J., Jiang, L., Cao, J. and Boullanger, P., "Influence of the Spacer Length of Glycolipid Receptors in Polydiacetylene Vesicles on the Colorimetric Detection of Escherichia coli," Langmuir, 16, 7801-7804(2000). https://doi.org/10.1021/la000126+
  14. Su, Y., Li, J., Jiang, L. and Cao, J., "Biosensor Signal Amplification of Vesicles Functionalized with Glycolipid for Colorimetric Detection of Escherichia coli," J. Colloid Interf. Sci., 284, 114-119(2005). https://doi.org/10.1016/j.jcis.2004.10.003
  15. Guo, C. X., Boullanger, P., Liu, T. and Jiang, L., "Size Effect of Polydiacetylene Vesicles Functionalized with Glycolipids on Their Colorimetric Detection Ability," J. Phy. Chem. B, 109, 18765-18771 (2005). https://doi.org/10.1021/jp052580y
  16. Lee, G. S., Hyun, S. J., Woo, S. M., Yang, K. J., Kim, J.-M. and Ahn, D. J., "Temperature-dependent Phase Behavior of Langmuir Films of 10,12-pentacosadiynoic Acid at the Air/water Interface and Its Effects on Chromatic Stability of the Polymerized Langmuir- Schaefer Films," Langmuir, submitted.
  17. Spevak, W., Nagy, J. O., Charych, D. H., Schaefer, M. E., Gilbert, J. H. and Bednarski, M. D., "Polymerized Liposomes Containing C-Glycosides of Sialic Acid: Potent Inhibitors of Influenza Virus in vitro Infectivity," J. Am. Chem. Soc., 115, 1146-1147(1993). https://doi.org/10.1021/ja00056a047
  18. Kim, J.-M., Lee, Y. B., Yang, D. H., Lee, J.-S., Lee, G. S. and Ahn, D. J., "A Polydiacetylene-Based Fluorescent Sensor Chip," J. Am. Chem. Soc., 127, 17580-17581(2005). https://doi.org/10.1021/ja0547275
  19. Kim, J.-M., Lee, J.-S. Lee, J.-S., Woo, S.-Y. and Ahn, D. J., "Unique Effects of Cyclodextrins on the Formation and Colorimetric Transition of Polydiacetylene Vesicles," Macromol. Chem. Phys., 206, 2299(2005). https://doi.org/10.1002/macp.200500331

Cited by

  1. Surface plasmon resonance study of (positive, neutral, negative) vesicles rupture by AgNPs’ attack for screening of cytotoxicity induced by nanoparticles vol.30, pp.2, 2013, https://doi.org/10.1007/s11814-012-0131-z