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http://dx.doi.org/10.9713/kcer.2011.49.4.400

Sensitivity Enhancement of Polydiacetylene Vesicles through Control of Particle Size and Polymerization Temperature  

Lee, Gil Sun (Department of Chemical and Biological Engineering, Korea University)
Oh, Jae Ho (Department of Chemical and Biological Engineering, Korea University)
Ahn, Dong June (Department of Chemical and Biological Engineering, Korea University)
Publication Information
Korean Chemical Engineering Research / v.49, no.4, 2011 , pp. 400-404 More about this Journal
Abstract
Many studies on polydiacetylene(PDA) have been investigated to apply to chemical and biological sensors due to their unique optical properties of color change from blue to red and fluorescence change from non-fluorescence to red fluorescence. Especially, high sensitivity against specific molecules is very important to apply polydiacetylenes to various sensors. In this study, we examined the effect of sensitivity enhancement of 10,12-pentacosadynoic acid(PCDA) vesicles in detection ${\alpha}$-cyclodextrin(CD) according to control of vesicle size by filters with different pore sizes and polymerization temperature. Colorimetric response(CR) was calculated using visible spectrometer. In order to investigate the effect of vesicle size on sensitivity of PDA vesicles, two PCDA vesicles were filtered without filtration and with 0.22 ${\mu}m$ filter. The two PCDA vesicles were polymerized at $25^{\circ}C$ and were incubated with ${\alpha}$-CD(5 mM) for 30 min. The CRs of the former and latter vesicles were 31.4% and 74.0%, respectively. Then, two PCDA vesicles filtered with 0.22 ${\mu}m$ filter were polymerized at $25^{\circ}C$ and $5^{\circ}C$ and were reacted with ${\alpha}$-CD(5 mM) for 30 min to examine the effect of polymerization temperature. The CRs of the former and latter vesicles were 74.0 and 99.2%, respectively. This suggests that vesicle sizes and polymerization temperature are key factors in enhancing the sensitivity of PDA vesicles. In addition, these results are expected to be useful to apply the PDA vesicles as biosensors to detect DNA, protein, and cells.
Keywords
Polydiacetylene Vesicles; Polymerization Temperature; Vesicle Size; Sensitivity Enhancement;
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Times Cited By KSCI : 1  (Citation Analysis)
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