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Simultaneous Determination of Vitamin A and E in Infant Formula by HPLC with Photodiode Array Detection

  • Lee, Hong-Min (Food Safety Center, Research and Development Institute, Namyang Dariy Co., Ltd.) ;
  • Kwak, Byung-Man (Food Safety Center, Research and Development Institute, Namyang Dariy Co., Ltd.) ;
  • Ahn, Jang-Hyuk (Food Safety Center, Research and Development Institute, Namyang Dariy Co., Ltd.) ;
  • Jeong, Seung-Hwan (Food Safety Center, Research and Development Institute, Namyang Dariy Co., Ltd.) ;
  • Shim, Sung-Lye (Department of Food and Nutrition, Chosun University) ;
  • Kim, Kyong-Su (Department of Food and Nutrition, Chosun University) ;
  • Yoon, Tae-Hyung (Nutrition and Functional Food Research Team, Korea Food and Drug Administration) ;
  • Leem, Dong-Gil (Nutrition and Functional Food Research Team, Korea Food and Drug Administration) ;
  • Jeong, Ja-Young (Nutrition and Functional Food Research Team, Korea Food and Drug Administration)
  • Received : 2010.09.20
  • Accepted : 2011.03.18
  • Published : 2011.04.30

Abstract

The objective of this study was to develop a method to simultaneously quantify vitamins A and E in infant formula. To determine the vitamin A and E content, vitamin A and four different vitamin E isomers (${\alpha}$-, ${\beta}$-, ${\gamma}$-, and ${\delta}$-tocopherol) were separated by high performance liquid chromatography with a photodiode array detector using a Develosil RPAQUEOUS RP-$C_{30}$ column ($4.6{\times}250$ mm, 5 ${\mu}M$). The vitamin A and E contents in the certified reference material determined using this method were within the certified range of standard values. The limits of detection (LODs) and limits of quantitation (LOQs) for vitamin A were 0.02 and 0.06 ${\mu}g/L$, respectively. LODs and LOQs for the vitamin E isomers ranged from 0.20 to 0.55 and from 0.67 to 1.81 ${\mu}g/L$, respectively. Linear analyses indicated that the square of the correlation coefficient for the vitamin A and E isomers was 0.9997-0.9999. The recovery of vitamins ranged from 96.69 to 97.79%. The results demonstrate that this novel method could be used to reliably analyze vitamin A and E content in infant formula.

Keywords

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