Reproductive and Developmental Biology

  • 제35권1호
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  • Pages.99-103
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  • 2011
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

돼지 난자의 체내 및 체외 성숙시 Transducin-like Enhancer Protein 1(TLE-1) mRNA의 발현

TLE-1 mRNA Expression during In Vivo and In Vitro Maturation in Porcine Oocytes

  • 장예진 (강원대학교 동물생명과학대학) ;
  • 김동우 (강원대학교 동물생명과학대학) ;
  • 이용승 (강원대학교 동물생명과학대학) ;
  • 정희태 (강원대학교 수의과대학) ;
  • 양부근 (강원대학교 동물생명과학대학) ;
  • 박춘근 (강원대학교 동물생명과학대학)
  • Jang, Ye-Jin (College of Animal Sciences, Kangwon National University) ;
  • Kim, Dong-Woo (College of Animal Sciences, Kangwon National University) ;
  • Lee, Yong-Seung (College of Animal Sciences, Kangwon National University) ;
  • Cheong, Hee-Tae (College Veterinary Medicine, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Sciences, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Sciences, Kangwon National University)
  • 투고 : 2011.02.26
  • 심사 : 2011.03.03
  • 발행 : 2011.03.31
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초록

Transducin-like enhancer protein 1(TLE-1) is protein associated with cell proliferation. This study analyzed change of TLE-1 mRNA expression during in vivo and in vitro maturation in porcine oocytes. Oocytes and granulose cells were collected from follicles of <2 mm, 2~6 mm and >6 mm in diameter in slaughtered pig's ovaries. Oocytes collected from follicles of 2~6 mm in diameter were used after in vitro maturation for 0, 10, 20 and 44 h. Cumulus cells and granulose cells were collected after treatment with hyaluronidase. In results, TLE-1 mRNA expression in oocytes collected from follicle >6 mm in diameter is increased, TLE-1 RNA expression in cumulus cells and granulosa cells from follicles <2 mm, 2 mm 6 mm and >6 mm in diameter. However, there is no significant difference. On the other hand, TLE-1 mRNA expression from oocytes cultured for 10 hand 44 h is increased, TLE-1 mRNA in cumulus cells cultured for 10 h is significant increased(p<0.05) than other culture periods. In conclusion, these results show that TLE-1 is expressed in all cell types of oocytes, cumulus cells and granulose cells, and associated with oocyte maturation.

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참고문헌

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