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TLE-1 mRNA Expression during In Vivo and In Vitro Maturation in Porcine Oocytes  

Jang, Ye-Jin (College of Animal Sciences, Kangwon National University)
Kim, Dong-Woo (College of Animal Sciences, Kangwon National University)
Lee, Yong-Seung (College of Animal Sciences, Kangwon National University)
Cheong, Hee-Tae (College Veterinary Medicine, Kangwon National University)
Yang, Boo-Keun (College of Animal Sciences, Kangwon National University)
Park, Choon-Keun (College of Animal Sciences, Kangwon National University)
Publication Information
Reproductive and Developmental Biology / v.35, no.1, 2011 , pp. 99-103 More about this Journal
Abstract
Transducin-like enhancer protein 1(TLE-1) is protein associated with cell proliferation. This study analyzed change of TLE-1 mRNA expression during in vivo and in vitro maturation in porcine oocytes. Oocytes and granulose cells were collected from follicles of <2 mm, 2~6 mm and >6 mm in diameter in slaughtered pig's ovaries. Oocytes collected from follicles of 2~6 mm in diameter were used after in vitro maturation for 0, 10, 20 and 44 h. Cumulus cells and granulose cells were collected after treatment with hyaluronidase. In results, TLE-1 mRNA expression in oocytes collected from follicle >6 mm in diameter is increased, TLE-1 RNA expression in cumulus cells and granulosa cells from follicles <2 mm, 2 mm 6 mm and >6 mm in diameter. However, there is no significant difference. On the other hand, TLE-1 mRNA expression from oocytes cultured for 10 hand 44 h is increased, TLE-1 mRNA in cumulus cells cultured for 10 h is significant increased(p<0.05) than other culture periods. In conclusion, these results show that TLE-1 is expressed in all cell types of oocytes, cumulus cells and granulose cells, and associated with oocyte maturation.
Keywords
In vivo/In vitro oocyte maturation; Granulosa cells; TLE-l mRNA; Pig;
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