The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Antifungal and Proteolytic Activity and Auxin Formation of Bacterial Strains Isolated from Highland Forest Soils of Halla Mountain

한라산 고지대 토양에서 분리한 미생물의 항균 및 단백질분해 활성, 오옥신 생산 특성

  • Kim, Tack-Soo (Applied Microbiology Laboratory, Agricultural Microbiology Team, NAAS, RDA) ;
  • Ko, Min-Jung (Applied Microbiology Laboratory, Agricultural Microbiology Team, NAAS, RDA) ;
  • Lee, Se-Weon (Applied Microbiology Laboratory, Agricultural Microbiology Team, NAAS, RDA) ;
  • Han, Ji-Hee (Applied Microbiology Laboratory, Agricultural Microbiology Team, NAAS, RDA) ;
  • Park, Kyung-Seok (Applied Microbiology Laboratory, Agricultural Microbiology Team, NAAS, RDA) ;
  • Park, Jin-Woo (Applied Microbiology Laboratory, Agricultural Microbiology Team, NAAS, RDA)
  • 김택수 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 고민정 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 이세원 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 한지희 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 박경석 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 박진우 (농촌진흥청 국립농업과학원 농업미생물팀)
  • Received : 2011.12.03
  • Accepted : 2011.12.22
  • Published : 2011.12.31
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Abstract

Bacterial strains were isolated from forest soils of Halla mountain, Jeju island in Korea. The soil samples were collected at each altitude of 100m from 1,000 m above sea level. Total 398 strains were isolated and tested for their physiological characteristics of antagonistic and proteolytic activities, and auxin production. Among the isolates, 172 strains were selected as antifungal strains showing antagonistic activity against at least one of 8 plant fungal pathogens (Alternaria alternata, Botrytis cinerea, Collectotrichum acutatum, Fusarium oxysporum, Phytophthora capsici, Pythium ultimum and Sclerotinia sclerotiorum). In addition 203 strains for proteolytic activity and 26 strains for auxin production were characterized for further study. Je28-4 (Rhodococcus sp.) were showed 80% of control value against tomato gray mold in vivo. Thus, it is suggested that soil bacteria isolated from forest soils of Halla mountain can be important sources of bioactive compounds for improving plant growth or promising biocontrol agents.

한라산에서 해발 1000미터부터 100미터의 고도 간격으로 산림토양 샘플을 채집하고 총 398점의 토양 세균을 분리하여 항균 및 단백질 분해활성과 Auxin 생산특성을 규명하였다. 항균활성 균주를 선발하기 위해 주요 작물병원균 8종에 대하여 대치배양한 결과 여러 종의 병원균에 대해 항균활성을 가지는 26균주를 1차적으로 선발하였으며, 단백질 분해활성이 높은 34균주, Auxin을 생산하는 26균주도 함께 선발하였다. 토마토잿빛곰팡이병에 대한 생물검정 결과 Je28-4(Rhodococcus sp.)가 80%의 방제효과를 나타내었다. 한라산에서 분리한 항균 및 단백질 분해활성, Auxin 생산균주 중 대표적인 균주를 선발하여 종을 동정한 결과 다양한 속에 속하는 미생물이 동정되었으며 Bacillus 속이 12균주로 가장 많았고 Streptomyces 8균주, Paenibacillus 속 5균주, Chryseobacterium 속 5균주, Pseudomonas 속 4균주, Rahnella 속 2균주, Lysinibacillus 속 2균주, Burkholderia 속 2균주로 동정되었으며, Enterobacter, Janthinobacterium, Microbacterium, Rhodococcus, Sphingomonas 속은 각각 1균주씩 동정되었다. 본 실험 결과 선발된 균주들은 추후 다각도의 in vitro 검정 및 생물검정을 걸쳐 식물 생장촉진 및 병 방제용 다기능성 미생물 자재의 소재로 활용 가능성을 검토할 필요가 있다고 사료된다.

Keywords

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