Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
권호 표지
DOI QR코드

DOI QR Code

Neuroprotective Effect of the n-Hexane Extracts of Laurus nobilis L. in Models of Parkinson's Disease

  • Ham, Ah-Rom (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Shin, Jong-Heon (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Oh, Ki-Bong (School of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Sung-Jin (Department of Animal Biotechnology, Kangwon National University) ;
  • Nam, Kung-Woo (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Koo, Uk (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Kim, Kyeong-Ho (College of Pharmacy, Kangwon National University) ;
  • Mar, Woong-Chon (Natural Products Research Institute, College of Pharmacy, Seoul National University)
  • Received : 2010.08.16
  • Accepted : 2010.10.18
  • Published : 2011.01.31
Download PDF
⟨ Previous Next ⟩

Abstract

Free radical scavenging and antioxidants have attracted attention as a way to prevent the progression of Parkinson's disease (PD). This study was carried out to investigate the effects of n-hexane fraction from Laurus nobilis L. (Lauraceae) leaves (HFL) on dopamine (DA)-induced intracellular reactive oxygen species (ROS) production and apoptosis in human neuroblastoma SH-SY5Y cells. Compared with apomorphine (APO, $IC_{50}=18.1\;{\mu}M$) as a positive control, the HFL $IC_{50}$ value for DA-induced apoptosis was $3.0\;{\mu}g/ml$, and two major compounds from HFL, costunolide and dehydrocostus lactone, were $7.3\;{\mu}M$ and $3.6\;{\mu}M$, respectively. HFL and these major compounds significantly inhibited ROS generation in DA-induced SH-SY5Y cells. A rodent 6-hydroxydopamine (6-OHDA) model of PD was employed to investigate the potential neuroprotective effects of HFL in vivo. 6-OHDA was injected into the substantia nigra of young adult rats and an immunohistochemical analysis was conducted to quantitate the tyrosine hydroxylase (TH)-positive neurons. HFL significantly inhibited 6-OHDA-induced TH-positive cell loss in the substantia nigra and also reduced DA induced $\alpha$-synuclein (SYN) formation in SH-SY5Y cells. These results indicate that HFL may have neuroprotective effects against DA-induced in vitro and in vivo models of PD.

Keywords

References

  1. Barzilai, A., Melamed, E. and Shirvan, A. (2001) Is there a rationale forneuroprotection against dopamine toxicity in Parkinson's disease?Cell. Mol. Neurobiol. 21, 215-235. https://doi.org/10.1023/A:1010991020245
  2. Chen, J. J. and Obering, C. (2005) A review of intermittent subcutaneousapomorphine injections for the rescue management of motorfluctuations associated with advanced Parkinson's disease. Clin.Ther. 27, 1710-1724. https://doi.org/10.1016/j.clinthera.2005.11.016
  3. Chu, Y. and Kordower, J. H. (2007) Age-associated increases of alpha-synuclein in monkeys and humans are associated with nigrostriataldopamine depletion: Is this the target for Parkinson's disease?Neurobiol. Dis. 25, 134-149. https://doi.org/10.1016/j.nbd.2006.08.021
  4. Chu, Y., Le, W., Kompoliti, K., Jankovic, J., Mufson, E. J. and Kordower,J. H. (2006) Nurr1 in Parkinson's disease and related disorders.J. Comp. Neurol. 494, 495-514. https://doi.org/10.1002/cne.20828
  5. Conforti, F., Statti, G., Uzunov, D. and Menichini, F. (2006) Comparativechemical composition and antioxidant activities of wild andcultivated Laurus nobilis L. leaves and Foeniculum vulgare subsp.piperitum (Ucria) coutinho seeds. Biol. Pharm. Bull. 29, 2056-2064 https://doi.org/10.1248/bpb.29.2056
  6. Dauer, W. and Przedborski, S. (2003) Parkinson's disease: mechanismsand models. Neuron 39, 889-909. https://doi.org/10.1016/S0896-6273(03)00568-3
  7. Dedov, V. N., Cox, G. C. and Roufogalis, B. D. (2001) Visualisation ofmitochondria in living neurons with single- and two-photon fluorescencelaser microscopy. Micron 32, 653-660. https://doi.org/10.1016/S0968-4328(00)00065-2
  8. Desplats, P., Lee, H. J., Bae, E. J., Patrick, C., Rockenstein, E., Crews,L., Spencer, B., Masliah, E. and Lee S. J. (2009) Inclusion formationand neuronal cell death through neuron-to-neuron transmissionof alpha-synuclein. Proc. Natl. Acad. Sci. USA. 106, 13010-13015. https://doi.org/10.1073/pnas.0903691106
  9. Golde, T. E. (2009) The therapeutic importance of understandingmechanisms of neuronal cell death in neurodegenerative disease.Mol. Neurodegener. 4, 8. https://doi.org/10.1186/1750-1326-4-8
  10. Gomez-Isla, T., Irizarry, M. C., Mariash, A., Cheung, B., Soto, O.,Schrump, S., Sondel, J., Kotilinek, L., Day, J., Schwarzschild, M.A., Cha, J. H., Newell, K., Miller, D. W., Ueda, K., Young, A. B.,Hyman, B. T. and Ashe, K. H. (2003) Motor dysfunction and gliosiswith preserved dopaminergic markers in human alpha-synucleinA30P transgenic mice. Neurobiol. Aging 24, 245-258. https://doi.org/10.1016/S0197-4580(02)00091-X
  11. Gomez-Santos, C., Ferrer, I., Santidrian, A. F., Barrachina, M., Gil, J.and Ambrosio, S. (2003) Dopamine induces autophagic cell deathand alpha-synuclein increase in human neuroblastoma SH-SY5Ycells. Neurosci. Res. 73, 341-350. https://doi.org/10.1002/jnr.10663
  12. Guo, S., Yan, J., Yang, T., Yang, X., Bezard, E. and Zhao, B. (2007)Protective effects of green tea polyphenols in the 6-OHDA rat modelof Parkinson's disease through inhibition of ROS-NO pathway.Biol. Psychiatry 62, 1353-1362. https://doi.org/10.1016/j.biopsych.2007.04.020
  13. Hughes, A. J., Bishop, S., Kleedorfer, B., Turjanski, N., Fernandez, W.,Lees, A. J. and Stern, G. M. (1993) Subcutaneous apomorphine inParkinson's disease: response to chronic administration for up tofive years. Mov. Disord. 8, 165-170. https://doi.org/10.1002/mds.870080208
  14. Khan, M. M., Ahmad, A., Ishrat, T., Khan, M. B., Hoda, M. N., Khuwaja,G., Raza, S. S., Khan, A., Javed, H., Vaibhav, K. and Islam,F. (2010) Resveratrol attenuates 6-hydroxydopamine-induced oxidativedamage and dopamine depletion in rat model of Parkinson'sdisease. Brain Res. 1328, 139-151. https://doi.org/10.1016/j.brainres.2010.02.031
  15. Kobayashi, H., Oikawa, S., Umemura, S., Hirosawa, I. and Kawanishi,S. (2008) Mechanism of metal-mediated DNA damage and apoptosisinduced by 6-hydroxydopamine in neuroblastoma SH-SY5Ycells. Free Radic. Res. 42, 651-660. https://doi.org/10.1080/10715760802270334
  16. Koch, E., Klaas, C. A., Rungeler, P., Castro, V., Mora, G., Vichnewski,W. and Merfort, I. (2001) Inhibition of inflammatory cytokine productionand lymphocyte proliferation by structurally different sesquiterpenelactones correlates with their effect on activation of NF-kappaB.Biochem. Pharmacol. 62, 795-801. https://doi.org/10.1016/S0006-2952(01)00714-6
  17. LeBel, C. P., Ischiropoulos, H. and Bondy, S. C. (1992) Evaluation ofthe probe 2',7'-dichlorofluorescin as an indicator of reactive oxygenspecies formation and oxidative stress. Chem. Res. Toxicol5, 227-231. https://doi.org/10.1021/tx00026a012
  18. Lo Bianco, C., Schneider, B. L., Bauer, M., Sajadi, A., Brice, A., Iwatsubo,T. and Aebischer, P. (2004) Lentiviral vector delivery of parkinprevents dopaminergic degeneration in an alpha-synuclein ratmodel of Parkinson's disease. Proc. Natl. Acad. Sci. USA. 101,17510-17515. https://doi.org/10.1073/pnas.0405313101
  19. Masliah, E., Rockenstein, E., Veinbergs, I., Mallory, M., Hashimoto, M.,Takeda, A., Sagara, Y., Sisk, A. and Mucke L. (2000) Dopaminergicloss and inclusion body formation in alpha-synuclein mice: implicationsfor neurodegenerative disorders. Science 287, 1265-1269. https://doi.org/10.1126/science.287.5456.1265
  20. Mattson, M. P. (2000) Apoptosis in neurodegenerative disorders. Nat.Rev. Mol. Cell Biol. 1, 120-129. https://doi.org/10.1038/35040009
  21. Paxinos, G. and Watson C. (1998) The rat brain. Academic Press.
  22. Sayyah, M., Saroukhani, G., Peirovi, A. and Kamalinejad, M. (2003)Analgesic and anti-inflammatory activity of the leaf essential oil ofLaurus nobilis Linn. Phytother. Res. 17, 733-736. https://doi.org/10.1002/ptr.1197
  23. Sayyah, M., Valizadeh, J. and Kamalinejad, M. (2002) Anticonvulsantactivity of the leaf essential oil of Laurus nobilis against pentylenetetrazole- and maximal electroshock-induced seizures. Phytomedicine9, 212-216. https://doi.org/10.1078/0944-7113-00113
  24. Tirmenstein, M. A., Hu, C. X., Scicchitano, M. S., Narayanan, P. K., Mc-Farland, D. C., Thomas, H. C. and Schwartz, L. W. (2005) Effectsof 6-hydroxydopamine on mitochondrial function and glutathionestatus in SH-SY5Y human neuroblastoma cells. Toxicol. In Vitro19, 471-479. https://doi.org/10.1016/j.tiv.2005.01.006
  25. Von Coelln, R., Kugler, S., Bahr, M., Weller, M., Dichgans, J. andSchulz, J. B. (2001) Rescue from death but not from functional impairment: caspase inhibition protects dopaminergic cells against6-hydroxydopamine-induced apoptosis but not against the loss oftheir terminals. J. Neurochem. 77, 263-273. https://doi.org/10.1046/j.1471-4159.2001.t01-1-00236.x
  26. Watanabe, H., Muramatsu, Y., Kurosaki, R., Michimata, M., Matsubara,M., Imai, Y. and Araki T. (2004) Protective effects of neuronalnitric oxide synthase inhibitor in mouse brain against MPTP neurotoxicity:an immunohistological study. Eur. Neuropsychopharmacol.14, 93-104. https://doi.org/10.1016/S0924-977X(03)00065-8
  27. Waxman, E. A. and Giasson, B. I. (2010) A novel, high-efficiency cellularmodel of fibrillar alpha-synuclein inclusions and the examinationof mutations that inhibit amyloid formation. J. Neurochem. 113,374-388. https://doi.org/10.1111/j.1471-4159.2010.06592.x
  28. Yoshikawa, M., Shimoda, H., Uemura, T., Morikawa, T., Kawahara, Y.and Matsuda, H. (2000) Alcohol absorption inhibitors from bay leaf (Laurus nobilis): structure-requirements of sesquiterpenes for theactivity. Bioorg Med. Chem. 8, 2071-2077. https://doi.org/10.1016/S0968-0896(00)00127-9
  29. Yuan, H., Liang, L. W., Chen, Z. J., Ji, H. R., Wang, M. K., Zhang,H. Y., Li, C. and Xu, J. Y. (2006) R-apomorphine protects against6-hydroxydopamine-induced nigrostriatal damage in rat. NeurosciBull. 22, 331-338.
  30. Zbarsky, V., Datla, K. P., Parkar, S., Rai, D. K., Aruoma, O. I. and Dexter,D. T. (2005) Neuroprotective properties of the natural phenolicantioxidants curcumin and naringenin but not quercetin and fisetinin a 6-OHDA model of Parkinson's disease. Free Radic. Res. 39,1119-1125. https://doi.org/10.1080/10715760500233113
  31. Zhang, Y. and Zhao, B. (2003) Green tea polyphenols enhance sodiumnitroprusside-induced neurotoxicity in human neuroblastoma SHSY5Ycells. J. Neurochem. 86, 1189-1200. https://doi.org/10.1046/j.1471-4159.2003.01928.x

Cited by

  1. Houttuyniae Herba protects rat primary cortical cells from Aβ25–35-induced neurotoxicity via regulation of calcium influx and mitochondria-mediated apoptosis vol.31, pp.7, 2012, https://doi.org/10.1177/0960327111433898
  2. The Na+/H+ exchanger-1 inhibitor cariporide prevents glutamate-induced necrotic neuronal death by inhibiting mitochondrial Ca2+ overload vol.90, pp.4, 2012, https://doi.org/10.1002/jnr.22818
  3. Neuroprotective effect of modified Chungsimyeolda-tang, a traditional Korean herbal formula, via autophagy induction in models of Parkinson׳s disease vol.159, 2015, https://doi.org/10.1016/j.jep.2014.11.007
  4. Dietary effect of dried bay leaves (Laurus nobilis ) meal on some biochemical parameters and on plasma oxidative status in New Zealand white growing rabbit vol.101, pp.5, 2017, https://doi.org/10.1111/jpn.12584
  5. Effects of magnolialide isolated from the leaves of Laurus nobilis L. (Lauraceae) on immunoglobulin E-mediated type I hypersensitivity in vitro vol.149, pp.2, 2013, https://doi.org/10.1016/j.jep.2013.07.015
  6. Bay Leaf : Potential Health Benefits vol.56, pp.4, 2011, https://doi.org/10.1097/nt.0000000000000493