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http://dx.doi.org/10.4062/biomolther.2011.19.1.118

Neuroprotective Effect of the n-Hexane Extracts of Laurus nobilis L. in Models of Parkinson's Disease  

Ham, Ah-Rom (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Shin, Jong-Heon (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Oh, Ki-Bong (School of Agricultural Biotechnology, Seoul National University)
Lee, Sung-Jin (Department of Animal Biotechnology, Kangwon National University)
Nam, Kung-Woo (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Koo, Uk (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Kim, Kyeong-Ho (College of Pharmacy, Kangwon National University)
Mar, Woong-Chon (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Publication Information
Biomolecules & Therapeutics / v.19, no.1, 2011 , pp. 118-125 More about this Journal
Abstract
Free radical scavenging and antioxidants have attracted attention as a way to prevent the progression of Parkinson's disease (PD). This study was carried out to investigate the effects of n-hexane fraction from Laurus nobilis L. (Lauraceae) leaves (HFL) on dopamine (DA)-induced intracellular reactive oxygen species (ROS) production and apoptosis in human neuroblastoma SH-SY5Y cells. Compared with apomorphine (APO, $IC_{50}=18.1\;{\mu}M$) as a positive control, the HFL $IC_{50}$ value for DA-induced apoptosis was $3.0\;{\mu}g/ml$, and two major compounds from HFL, costunolide and dehydrocostus lactone, were $7.3\;{\mu}M$ and $3.6\;{\mu}M$, respectively. HFL and these major compounds significantly inhibited ROS generation in DA-induced SH-SY5Y cells. A rodent 6-hydroxydopamine (6-OHDA) model of PD was employed to investigate the potential neuroprotective effects of HFL in vivo. 6-OHDA was injected into the substantia nigra of young adult rats and an immunohistochemical analysis was conducted to quantitate the tyrosine hydroxylase (TH)-positive neurons. HFL significantly inhibited 6-OHDA-induced TH-positive cell loss in the substantia nigra and also reduced DA induced $\alpha$-synuclein (SYN) formation in SH-SY5Y cells. These results indicate that HFL may have neuroprotective effects against DA-induced in vitro and in vivo models of PD.
Keywords
Laurus nobilis L; Dopamine; Parkinson's disease; Neuroprotective; $\alpha$-synuclein;
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