Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Evaluation of Anti-Colitic Effect of Chung-Jang-Hwan (C-mix) in Mice

  • Received : 2010.08.06
  • Accepted : 2010.11.02
  • Published : 2011.01.31
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Abstract

The inhibitory effect of Chung-Jang-Hwan (C-mix) consisted of Geranium nepalense subsp. thunbergii, Saururus chinensis, and Rubus coreanus were investigated in dextran sulfate sodium (DSS)-induced colitic mice by microarray analysis. Treatment with Cmix improved colitic symptoms, including colon shortening and myeloperoxidase activity. Treatment with DSS alone upregulated the expression levels of inflammation-related genes, including IL-$1\beta$, IL-6, CCL2, CCL4, CCL5, CCL7, CCL8, CCL24, CXCL1, CXCL2, CXCL5, CXCL9 and CXCL10, and other colitis-related genes such as COX-2, PAP, MMP family, S100a8, S100a9 and DEFA1 in mice. However, treatment with C-mix inhibited the expression levels of inflammation-associated genes induced by DSS. The increased expression levels of COX-2 and IL-$1\beta$, representative inflammatory genes, were confirmed by a quantitative realtime polymerase chain reaction analysis. These results indicate that C-mix may ameliorate colitis by the inhibitory regulation of inflammation-associated genes.

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References

  1. Amoli, M., Larijani, B., Thomson, W., Ollier, W. and Gonzalez-Gay, M.(2005) Two polymorphisms in the epithelial cell-derived neutrophil-activatingpeptide (ENA-78) gene. Dis. Markers 21, 75-77. https://doi.org/10.1155/2005/864525
  2. Atreya, R., Mudter, J., Finotto, S., Müllberg, J., Jostock, T., Wirtz, S.,Schütz, M., Bartsch, B., Holtmann, M., Becker, C., Strand, D.,Czaja, J., Schlaak, J. F., Lehr, H. A., Autschbach, F., Schürmann,G., Nishimoto, N., Yoshizaki, K., Ito, H., Kishimoto, T., Galle, P. R.,Rose-John, S. and Neurath, M. F. (2000) Blockade of interleukin6 trans signaling suppresses T-cell resistance against apoptosisin chronic intestinal infl ammation: evidence in crohn disease andexperimental colitis in vivo. Nat. Med. 6, 583-588. https://doi.org/10.1038/75068
  3. Autschbach, F., Giese, T., Gassler, N., Sido, B., Heuschen, G., Heuschen,U., Zuna, I., Schulz, P., Weckauf, H., Berger, I., Otto, H.F. and Meuer, S. C. (2002) Cytokine/chemokine messenger-RNAexpression profi les in ulcerative colitis and Crohn's disease. VirchowsArch. 441, 500-513. https://doi.org/10.1007/s00428-002-0684-z
  4. Baugh, M., Perry, M., Hollander, A., Davies, D., Cross, S., Lobo, A.,Taylor, C. J. and Evans, G. S. (1999) Matrix metalloproteinase levelsare elevated in infl ammatory bowel disease. Gastroenterology117, 814-822. https://doi.org/10.1016/S0016-5085(99)70339-2
  5. Binder, V. (2004) Epidemiology of IBD during the twentieth century: anintegrated view. Best Pract. Res. Clin. Gastroenterol. 18, 463-479. https://doi.org/10.1016/j.bpg.2003.12.002
  6. Cole, A., Ganz, T., Liese, A., Burdick, M., Liu, L. and Strieter, R. (2001)Cutting edge: IFN-inducible ELR- CXC chemokines display defensin-like antimicrobial activity. J. Immunol. 167, 623-7. https://doi.org/10.4049/jimmunol.167.2.623
  7. Costello, C., Mah, N., Hasler, R., Rosenstiel, P., Waetzig, G., Hahn,A., Lu, T., Gurbuz, Y., Nikolaus, S., Albrecht, M., Hampe, J., Lucius,R., Kloppel, G., Eickhoff, H., Lehrach, H., Lengauer, T. andSchreiber, S. (2005) Dissection of the infl ammatory bowel diseasetranscriptome using genome-wide cDNA microarrays. PLoS Med.2, e199. https://doi.org/10.1371/journal.pmed.0020199
  8. Dieckgraefe, B., Crimmins, D., Landt, V., Houchen, C., Anant, S.,Porche-Sorbet, R. and Ladenson, J. H. (2002) Expression of theregenerating gene family in infl ammatory bowel disease mucosa:Reg Ialpha upregulation, processing, and antiapoptotic activity. J.Investig. Med. 50, 421-434. https://doi.org/10.2310/6650.2002.32518
  9. Egesten, A., Eliasson, M., Olin, A., Erjefält, J., Bjartell, A., Sangfelt,P. and Carlson, M. (2007) The proinfl ammatory CXC-chemokinesGRO-alpha/CXCL1 and MIG/CXCL9 are concomitantly expressedin ulcerative colitis and decrease during treatment with topical corticosteroids.Int. J. Colorectal. Dis. 22, 1421-1427. https://doi.org/10.1007/s00384-007-0370-3
  10. Egesten, A., Olin, A., Linge, H., Yadav, M., Morgelin, M., Karlsson, A.and Collin, M. (2009) SpeB of Streptococcus pyogenes differentiallymodulates antibacterial and receptor activating properties ofhuman chemokines. PLoS One 4, e4769. https://doi.org/10.1371/journal.pone.0004769
  11. Fukata, M., Chen, A., Klepper, A., Krishnareddy, S., Vamadevan,A., Thomas, L., Xu, R., Inoue, H., Arditi, M., Dannenberg, A. J.and Abreu, M. T. (2006) Cox-2 is regulated by Toll-like receptor-4(TLR4) signaling: Role in proliferation and apoptosis in the intestine.Gastroenterology 131, 862-877. https://doi.org/10.1053/j.gastro.2006.06.017
  12. Helwig, U., Gionchetti, P., Rizzello, F., Lammers, K., Kuhbacher, T.,Schreiber, S., Baggiolini, M., Uguccioni, M. and Campieri, M.(2004) CXC and CC chemokine expression in inflamed and noninflamed pelvic ileal pouch tissue. Int. J. Colorectal. Dis. 19, 165-170. https://doi.org/10.1007/s00384-003-0500-5
  13. Jonsson, D., Amisten, S., Bratthall, G., Holm, A. and Nilsson, B. (2009)LPS induces GROalpha chemokine production via NF-kappaB inoral fibroblasts. Inflamm. Res. 58, 791-796. https://doi.org/10.1007/s00011-009-0049-z
  14. Khan, I., Al-Awadi, F., Thomas, N., Haridas, S. and Anim, J. (2002)Cyclooxygenase-2 inhibition and experimental colitis: beneficial effectsof phosphorothioated antisense oligonucleotide and meloxicam.Scand. J. Gastroenterol. 37, 1428-1436. https://doi.org/10.1080/003655202762671314
  15. Kitamura, K., Nakamoto, Y., Kaneko, S. and Mukaida, N. (2004) Pivotalroles of interleukin-6 in transmural infl ammation in murine Tcell transfer colitis. J. Leukoc. Biol. 76, 1111-1117. https://doi.org/10.1189/jlb.0604328
  16. Kobayashi, Y. (2008) The role of chemokines in neutrophil biology.Front Biosci. 13, 2400-2407. https://doi.org/10.2741/2853
  17. Kwon, J., Keates, A., Anton, P., Botero, M., Goldsmith, J. and Kelly,C. (2005) Topical antisense oligonucleotide therapy against LIX,an enterocyte-expressed CXC chemokine, reduces murine colitis.Am. J. Physiol. Gastrointest. Liver Physiol. 289, G1075-1083. https://doi.org/10.1152/ajpgi.00073.2005
  18. Lawrance, I., Fiocchi, C. and Chakravarti, S. (2001) Ulcerative colitisand Crohn's disease: distinctive gene expression profi les and novelsusceptibility candidate genes. Hum. Mol. Genet. 10, 445-456. https://doi.org/10.1093/hmg/10.5.445
  19. Lee, J., Hwang, B., Kim, K., Nam, J. and Hong, Y. and Lee, J. (2003)Suppression of RelA/p65 transactivation activity by a lignoidmanassantin isolated from Saururus chinensis. Biochem. Pharmacol.66, 1925-1933. https://doi.org/10.1016/S0006-2952(03)00553-7
  20. Lobenhofer, E., Bushel, P., Afshari, C. and Hamadeh, H. (2001) Progressin the application of DNA microarrays. Environ. Health Perspect.109, 881-891. https://doi.org/10.1289/ehp.01109881
  21. Louis, E., Ribbens, C., Godon, A., Franchimont, D., De Groote, D.,Hardy, N., Boniver, J., Belaiche, J. and Malaise, M. (2000) Increasedproduction of matrix metalloproteinase-3 and tissue inhibitorof metalloproteinase-1 by infl amed mucosa in infl ammatorybowel disease. Clin. Exp. Immunol. 120, 241-246. https://doi.org/10.1046/j.1365-2249.2000.01227.x
  22. McCormack, G., Moriarty, D., O'Donoghue, D., McCormick, P., Sheahan,K. and Baird, A. (2001) Tissue cytokine and chemokine expression in inflammatory bowel disease. Inflamm. Res. 50, 491-495. https://doi.org/10.1007/PL00000223
  23. Ogawa, H., Fukushima, K., Naito, H., Funayama, Y., Unno, M., Takahashi,K., Kitayama, T., Matsuno, S., Ohtani, H., Takasawa, S.,Okamoto, H. and Sasaki, I. (2003) Increased expression of HIP/PAP and regenerating gene III in human inflammatory bowel diseaseand a murine bacterial reconstitution model. Inflamm. BowelDis. 9, 162-170. https://doi.org/10.1097/00054725-200305000-00003
  24. Pallone, F. and Monteleone, G. (1998) Interleukin 12 and Th1 responsesin inflammatory bowel disease. Gut 43, 735-736. https://doi.org/10.1136/gut.43.6.735
  25. Papadakis, K. (2004) Chemokines in inflammatory bowel disease.Curr. Allergy Asthma Rep. 4, 83-89. https://doi.org/10.1007/s11882-004-0048-7
  26. Park, J., Oh, S., Lim, S., Lee, Y., Shin, H., Oh, Y., Choe, N. H., Park,J. H. and Kim, J. K. (2006) Induction of heme oxygenase-1 mediatesthe anti-inflammatory effects of the ethanol extract of Rubuscoreanus in murine macrophages. Biochem. Biophys. Res. Commun.351, 146-152. https://doi.org/10.1016/j.bbrc.2006.10.008
  27. Rafii, F., Ruseler-Van Embden, J. and van Lieshout, L. (1999) Changes in bacterial enzymes and PCR profiles of fecal bacteria from a patient with ulcerative colitis before and after antimicrobial treatments. Dig. Dis. Sci. 44, 637-642 https://doi.org/10.1023/A:1026634229934
  28. Salmela, M., MacDonald, T., Black, D., Irvine, B., Zhuma, T., Saarialho-Kere, U. and Pender, S. L. (2002) Upregulation of matrix metalloproteinasesin a model of T cell mediated tissue injury in the gut:analysis by gene array and in situ hybridisation. Gut 51, 540-547. https://doi.org/10.1136/gut.51.4.540
  29. Sasaki, S., Yoneyama, H., Suzuki, K., Suriki, H., Aiba, T., Watanabe,S., Kawauchi, Y., Kawachi, H., Shimizu, F., Matsushima, K, Asakura,H, and Narumi, S. (2002) Blockade of CXCL10 protects micefrom acute colitis and enhances crypt cell survival. Eur. J. Immunol.32, 3197-3205. https://doi.org/10.1002/1521-4141(200211)32:11<3197::AID-IMMU3197>3.0.CO;2-1
  30. Schreiber, S., Nikolaus, S., Hampe, J., Hamling, J., Koop, I., Groessner,B., Lochs, H. and Raedler, A. (1999) Tumour necrosis factoralpha and interleukin 1beta in relapse of Crohn's disease. Lancet.353, 459-461. https://doi.org/10.1016/S0140-6736(98)03339-X
  31. Shanahan, F. (2002) Crohn's disease. Lancet 359, 62-69. https://doi.org/10.1016/S0140-6736(02)07284-7
  32. Shimoyama, T., Sawada, K., Hiwatashi, N., Sawada, T., Matsueda,K., Munakata, A., Asakura, H., Tanaka, T., Kasukawa, R., Kimura,K., Suzuki, Y., Nagamachi, Y., Muto, T., Nagawa, H., Iizuka, B.,Baba, S., Nasu, M., Kataoka, T., Kashiwagi, N. and Saniabadi, A.R. (2001) Safety and efficacy of granulocyte and monocyte adsorptionapheresis in patients with active ulcerative colitis: a multicenterstudy. J. Clin. Apher. 16, 1-9. https://doi.org/10.1002/jca.1000
  33. Singleton, V. L., Orthofer, R. and Lamuela-Raventos, R. M. (1999)Analysis of total phenols and other oxidation substrates and antioxidantsby means of Folin-Ciocalteu reagent. Oxidants and Antioxidants,Pt A. 299, 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
  34. Szkaradkiewicz, A., Marciniak, R., Chudzicka-Strugała, I., Wasilewska,A., Drews, M., Majewski, P., Karpinski, T. and Zwozdziak, B.(2009) Proinfl ammatory cytokines and IL-10 in infl ammatory boweldisease and colorectal cancer patients. Arch. Immunol. Ther. Exp.(Warsz) 57, 291-294. https://doi.org/10.1007/s00005-009-0031-z
  35. te Velde, A., de Kort, F., Sterrenburg, E., Pronk, I., ten Kate, F., Hommes,D. and van Deventer, S. J. (2007) Comparative analysisof colonic gene expression of three experimental colitis modelsmimicking inflammatory bowel disease. Inflamm. Bowel Dis. 13,325-330 https://doi.org/10.1002/ibd.20079
  36. Tipoe, G., White, F. and Pritchett, C. (1992) A morphometric study ofhistological variations during cellular differentiation of normal humancolorectal epithelium. J. Anat. 181(Pt 2), 189-197.
  37. Uguccioni, M., Gionchetti, P., Robbiani, D., Rizzello, F., Peruzzo, S.,Campieri, M. and Baggiolini, M. (1999) Increased expression ofIP-10, IL-8, MCP-1, and MCP-3 in ulcerative colitis. Am. J. Pathol.155, 331-336. https://doi.org/10.1016/S0002-9440(10)65128-0
  38. Xiufen, W., Hiramatsu, N. and Matsubara, M. (2004) The antioxidativeactivity of traditional Japanese herbs. Biofactors 21, 281-284. https://doi.org/10.1002/biof.552210155
  39. Yang, H., Oh, S., Lim, S., Shin, H., Oh, Y. and Kim, J. (2008) Antiinflammatory activities of Rubus coreanus depend on the degree offruit ripening. Phytother. Res. 22, 102-107. https://doi.org/10.1002/ptr.2274
  40. Yang, S, Choi, M, Kim, O, Myung, S, Jung, H, Hong, W., Kim, J. H.,and Min, Y. I. (2002) The increased expression of an array of C-X-C and C-C chemokines in the colonic mucosa of patients withulcerative colitis: regulation by corticosteroids. Am. J. Gastroenterol.97, 126-132. https://doi.org/10.1111/j.1572-0241.2002.05431.x
  41. Yoo, H., Kang, H., Jung, H., Kim, K., Lim, C. and Park, E. (2008)Anti-inflammatory, anti-angiogenic and anti-nociceptive activitiesof Saururus chinensis extract. J. Ethnopharmacol. 120, 282-286. https://doi.org/10.1016/j.jep.2008.08.016
  42. Zhong, W., Kolls, J., Chen, H., McAllister, F., Oliver, P. and Zhang, Z.(2008) Chemokines orchestrate leukocyte trafficking in inflammatorybowel disease. Front Biosci. 13, 1654-1664. https://doi.org/10.2741/2789

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