Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of Growth Inhibition and Isolation of a Copper-Resistant Rhizobacterium, Alcaligenes sp. KC-1

Cu 내성 근권 세균 Alcaligenes sp. KC-1의 분리 및 생장특성

  • Hong, Sun-Hwa (Department of Environmental and Energy Engineering, University of Suwon) ;
  • Shin, Ki-Chul (Department of Environmental and Energy Engineering, University of Suwon) ;
  • Lee, Eun-Young (Department of Environmental and Energy Engineering, University of Suwon)
  • 홍선화 (수원대학교 환경에너지공학과) ;
  • 신기철 (수원대학교 환경에너지공학과) ;
  • 이은영 (수원대학교 환경에너지공학과)
  • Received : 2011.05.23
  • Accepted : 2011.06.12
  • Published : 2011.06.28
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Abstract

In this study, A bacterium with an ability to resist toxic heavy metals was isolated from reeds in wetland. The isolated strain was identified to Alcaligenes sp. KC-1 by 16S rDNA sequencing. Heavy metals such as Pb, $Cr^{6+}$, Cd, Zn and Cu were supplied to media. The ecotoxic treat of the heavy metals on the growth of strain KC-1 was performed when the isolated strain Alcaligenes sp. KC-1 cultured with Cu ranging from 0 mM to 20 mM. It showed the resistance of $EC_{50}$(7.34 mM) and cell growth ($OD_{600\;nm}$ : 0.83 after 42 hours) when it was cultured in Cu.

습지의 갈대토양으로부터 중금속에 내성이 있는 세균이 분리되었다. 분리된 균주는 16S rDNA 염기서열분석에 의거하여 Alcaligenes sp.로 동정되었다. 납, 크롬, 카드뮴, 아연 및 구리와 같은 중금속을 배지에 첨가하였다. 분리균주 Alcaligenes sp. KC-1을 구리가 0 mM에서 20 mM의 농도로 첨가된 배지에서 배양하였을 때 균주의 생장에 미치는 독성을 알아보았다. 분리균주는 구리가 존재할 때 42시간 배양된 후 7.34 mM의 $EC_{50}$값과 $OD_{600\;nm}$에서 0.83의 흡광도 값을 보여주는 구리 내성균주였다.

Keywords

References

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