Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antibacterial Activity of Silver-nanoparticles Against Staphylococcus aureus and Escherichia coli

황색 포도상구균과 대장균에 대한 은나노 입자의 항균활성

  • Kim, Soo-Hwan (Department of Smart Foods and Drugs, Inje University) ;
  • Lee, Hyeong-Seon (Department of Smart Foods and Drugs, Inje University) ;
  • Ryu, Deok-Seon (Department of Smart Foods and Drugs, Inje University) ;
  • Choi, Soo-Jae (Department of Smart Foods and Drugs, Inje University) ;
  • Lee, Dong-Seok (Department of Smart Foods and Drugs, Inje University)
  • 김수환 (인제대학교 식의약생명공학과) ;
  • 이형선 (인제대학교 식의약생명공학과) ;
  • 류덕선 (인제대학교 식의약생명공학과) ;
  • 최수재 (인제대학교 식의약생명공학과) ;
  • 이동석 (인제대학교 식의약생명공학과)
  • Received : 2010.12.16
  • Accepted : 2011.02.25
  • Published : 2011.03.28
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Abstract

The antibacterial activities of silver nanoparticles (Ag-NPs) were studied with respect to Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli by observing the bacterial cells treated or not with Ag-NPs by FE-SEM as well as measuring the growth curves, formation of bactericidal ROS, protein leakage, and lactate dehydrogenase activity involved in the respiratory chain. Bacterial cells were treated with Ag-NPs powder, and the growth rates were investigated under varying concentrations of Ag-NPs, incubation times, incubation temperatures, and pHs. As a result, S. aureus and E. coli were shown to be substantially inhibited by Ag-NPs, and the antibacterial activity of Ag-NPs did not fluctuate with temperature or pH. These results suggest that Ag-NPs could be used as an effective antibacterial material.

본 연구는 은나노 입자의 항균활성을 알아보기 위하여, 그람 양성세균인 황색포도상구균과 그람 음성세균인 대장균에 대한 은나노 입자(Ag-NPs)를 처리 후, 세균세포 생장곡선측정, 활성산소생성능 측정, 세포질 단백질 누출량 측정, 젖산탈수소효소 활성측정 및 고분해능 임계방사 주사전자현미경 관찰이 수행되었다. 세균세포의 생장곡선 측정은 다양한 농도, 배양시간, 배양온도 및 pH에서 수행되었다. 결과적으로 황색 포도상구균과 대장균은 배양온도와 pH에 영향을 받지않고 은나노 입자에 의해 효과적으로 생장억제가 이루어지는 것을 관찰할 수 있었다. 또한 활성산소의 생성에 의하여 세포막의 파괴로 세포질내 물질의 유출을 세포질 유래 단백질 측정으로 확인할 수 있었으며, 젖산탈수소효소 활성측정을 통하여 은나노 입자에 대한 세포호흡억제활성 또한 확인할 수 있었다. 임계방사 주사전자현미경 관찰결과 은나노 입자에 의한 세균 세포표면의 형태학적 변화 또한 관찰되었다. 이러한 결과를 통하여 은나노 입자를 효과적인 항균활성소재로 활용 가능함이 입증되었다.

Keywords

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